Authors:Halil Gokce, Oktay Genc, Atila Akca, Zati Vatansever, Ahmet Unver, and Hidayet Erdogan
de la Fuente, J., Torina, A., Caracappa, S., Tumino, G., Furla, R., Almazan, C. and Kocan, K. M. (2005
): Serologic and molecular characterization of
species infection in farm animals and ticks from Sicily. Vet. Parasitol.
ELISAs to differentiate between Aujeszky's disease-vaccinated and infected animals. J. Virol. Meth. 65, 83--94.
Glycoprotein gE blocking ELISAs to differentiate between Aujeszky's disease-vaccinated and infected animals
Authors:J. Rajčáni, N. Ásványi-Molnár, and S. Szathmary
Rajčáni, J., Kúdelová, M.: Murid herpesvirus 4 (MuHV-4): An animal model for human gammaherpesvirus research. In Minarovits, J. et al. (eds): Latency Strategies of Herpesviruses. Springer, 2007, pp. 102–136.
Various animal models in farm and laboratory animals indicate the possible pathological effect of soybean feeding. Immunity and tolerance to oral soybean intake and a generalised meta-model of effects are discussed. It seems that FAO/WHO recommendations will have to be considered in assessing the biological value of soybean feed sources. Determination of hull content, antigenic potential, conglycinin and trypsin inhibitor content is warranted in view of the future component pricing of soybean meals.
Authors:I. Kiss, S. Kecskeméti, J. Tanyi, S. Belák, C. Ros, and S. B. Klingeborn
The quasispecies nature of three animal pathogenic RNA viruses of field origin was examined by testing variants of classical swine fever virus (CSFV) originating from geographically different areas, feline coronavirus (FCoV) detected from the same animal by successive sampling, and rabbit haemorrhagic disease virus (RHDV) originating from successive outbreaks in the same geographic area. Clinical samples were investigated using reverse transcriptase polymerase chain reaction (RT-PCR) and ensuing single strand conformational polymorphism (SSCP) assay. By the combination of these methods even subtle differences could be detected among the amplified fragments of the same virus species of different origin. FCoV proved to comprise the most and CSFV the less heterogeneous virus quasispecies. The results show that the combination of RT-PCR and SSCP provides novel and highly sensitive means for the characterisation of RNA viruses, with special regard to genome composition, evolution, features of pathogenicity and molecular epizootiology.
Authors:J. Domahidi, Péter Katica, and Judit Farkas
Domahidi, I., Egyed-Zsigmond, I., Kolozsvári, É., Peti, A.: The acute effect and histopathological changes produced by methol in experimental animals, (in Romanian), Revista Românâ de Medicina Muncii 48 (3-4), 10015-10019 (1998
The proportion of Escherichia coli non-susceptible to 3rd generation cephalosprins from invasive clinical samples has risen in Hungary from 5.1 per cent in 2006 to 15.5 per cent in 2011. The prevalence of ESBL-production in E. coli of animal origin remains unknown. During the first stage of a probe forty-five human and 18 animal ESBL-producing E. coli strains isolated in 2006-2007 were investigated. The human strains were representatively selected from a collection of 113 ESBL-producing isolates sent to the national reference center from local laboratories across the country. A variety of ESBLs were detected (SHV-2, -5, -12, CTX-M-32) with CTX-M-15 being the most common in human and CTX-M-1 the dominant in animal isolates. Genetic characterization revealed that thirty-six human isolates (80 per cent) belonged to either the phylogenetic group (PG) B2 or D. Conversely, 15 animal isolates (83 per cent) proved to be members of the A and B1 commensal PGs. Furthermore 46 per cent of human isolates (21/45) from 12 centres belonged to the international O25-ST131/B2 clone while nine isolates from seven centers showed the O15 serotype. Pulsed-field gel electrophoresis (PFGE) detected 22 and 11 diverse pulsotypes among 45 human and 18 animal isolates, respectively. The human and animal strains did not share any pulsotypes.