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collection and determination of tear MMP-9 concentration using an enzyme-linked immunosorbent assay (ELISA) kit The schedule of tear collection was set up as per the degree of corneal re-epithelialisation using a fluorescein staining test. The fluorescence
indicated with a dark coloured background and the names of the three counties Serological methods Serum samples were tested by ELISA method using the HEV Ab (version Ultra, Dia.Pro Diagnostic Bioprobes, Sesto San Giovanni, Italy) and HEV IgM (Dia
other abnormalities and diseases. None of them was pregnant neither was any on contraceptives. Laboratory tests ITIH4 and haptoglobin were measured using two ELISA Sandwich tests (Acuvet Biotech, Zaragoza, Spain) that were previously developed and
analysis These samples were tested for the presence of group-specific antibodies against BTV using a commercially available competitive enzyme linked immunosorbent assay (c-ELISA) (ID Screen Bluetongue Competition ELISA, IDvet, France) as per manufacturer
Antibodies on the SARS-CoV-2 virus were detected using the automated “Sandwich” enzyme-linked immunosorbent assay (ELISA) method with Euroimmun ELISA Analyser I-2P (EUROIMMUN Medizinische Labordiagnostika AG, Lübeck, Germany). The samples were tested using
–3 2,600 54 7 825 266 3.1 9 (3.4%) 14 (1.7%) 1–2 1,700 49 8 665 253 2.6 8 (3.2%) 13 (2.0%) 1–3 1,200 55 Total 16,589 3,393 4.9 127 (3.7%) 291 (1.8%) The sera were tested by indirect ELISA (iELISA) for the presence of serum antibodies against S. equi
using the enzyme-linked immunosorbent assay using the Human SERPINA4 (Kallistatin) ELISA Kit (Elabscience, USA). The level of C-reactive protein (CRP) was determined using the hs-CRP ELISA Kit (Biomerica USA). Based on the obtained clinical
participate in the study. We determined serum fetuin-A concentration by radial immunodiffusion, as described earlier [ 21 ]. Anti- Helicobacter IgG was determined by ELISA using the NovaLisa kit (NovaTec, Dietzenbach, Germany). Values ≤1.0 were
a freezer (−20 °C) for storage. The concentration of melatonin was determined by a universal Melatonin ELISA Kit (ab285251, Abcam plc, Cambridge, UK; analytical sensitivity 4.7 pg mL −1 ; range 7.8–500 pg mL −1 ; intra-assay precision <5%; inter
measurement of IL-6 was performed in duplicate in the blood samples by using an enzyme immunoassay with Human Il-6 ELISA kit (Elabscience Biotechnology Inc. USA). The detection range was 7.81–500 pg/mL, with intra and interassay coefficients of variation below
