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Abstract  

The concentrations of ten trace elements were assayed up to 3 hours after an infusion of an amino acid mixture. The changes in metabolism induced by the administered amino acids led to characteristic alterations of the trace element concentrations. These characteristic alterations could be explained by a mechanism which seems to compensate for membraneous charge alterations caused by the metabolic reaction. Obviously, non essential trace elements are involved in this mechanism.

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Acta Chromatographica
Authors: K. Wróblewski, A. Petruczynik, B. Buszewski, M. Szultka-Młyńska, H. Karakuła-Juchnowicz, and M. Waksmundzka-Hajnos

Vortioxetine is a new drug against major depressive disorder with high affinity for a range of different serotonergic targets in the central nervous system. Therapeutic drug monitoring is an important tool for the clinical management of patients receiving a pharmacotherapy, particularly in psychiatry. For this reason, determination of drug concentration in biological fluids is important for a rational dosage of drugs. Rapid and reliable analytical assays are also required to detect and identify drugs of toxicological importance. For analysis of vortioxetine by high-performance liquid chromatography (HPLC), no procedures for its determination in saliva have been reported and there are only a few ones for its determination in serum. A sensitive and selective highperformance liquid chromatography with diode array detector (HPLC-DAD) or mass spectrometer (HPLC-MS) method was developed for the fast quantification of vortioxetine in human saliva and serum. The determination was performed on a Synergi Polar RP column in isocratic mode under the optimal mobile phase containing 70% methanol, 20% acetate buffer at pH 3.5, 10% double distilled water, and 0.025 M L−1 diethylamine.

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Abstract  

Kits containing macroaggregated albumin were prepared and compared with three commercial kits with respect to particle size distribution, radiochemical yield and biodistribution. Our preparation was comparable to the commercial products.

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Abstract  

The objective of the present study was oriented to produce and purify polyclonal anti-PRL antibody as the main key store in immunoradiometric assay (IRMA) using solid phase cellulose particles for determination of PRL in human sera. The preparation of 125I-PRL was carried out by lactoperoxidase method for estimation of the titre of antibody production. The preparation of standards was undertaken. The activation of cellulose particles using 1,1-carbonyl diimidazole (CDI) and coupling of these solid phase particles with purified Rabbit anti-PRL were carried out. Optimization and validation of the assay were carried out. Results revealed that the produced PRL polyclonal antibodies have high titre. Cellulose particles IRMA system was highly sensitive and specific. The intra- and inter-assay variations were satisfactory. The recovery and dilution tests indicated accurate calibration and appropriate matrix. The present technique agreed well with IRMA commercial kit. These cellulose particles retained their characteristics during storage for 6 months at 4 °C. In conclusion, this low cost assay could be used as a useful diagnostic tool for diagnosis and follow up of galactorrhea, infertility and pituitary adenoma.

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behavior. The effect of the concentration of IL or acetonitrile in mobile phases and effect of temperature was investigated and optimal eluent for analysis of psychotropic drugs in human serum was chosen. Experimental

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by Wako Pure Chemical Industries Ltd. Human serum was purchased from MP Biomedicals Inc., and serum samples obtained from the patient were maintained at −20°C until required for analysis. MonoSpin C 18 and C 18 -CX were purchased from GL Science

Open access
Acta Chromatographica
Authors: Fatema Moni, Suriya Sharmin, Satyajit Roy Rony, Farhana Afroz, Shammi Akhter, and Md. Hossain Sohrab

, accurate, and precise method for determination of Esomeprazole in human serum using HPLC suitable for Pharmacokinetic study, Bioavailability, and Bioequivalence study. Experimental Chemicals Esomeprazole and Pantoprazole (Internal standard, IS) were kindly

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detection. Sample analysis In order to verify the feasibility of the established methods, the experiments were performed on spiked recovery tests in human serum and urine. Fig. 5 and Fig. 6 show the chromatograms and electropherograms of serum and urine

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Calorimetric characterization of 2′,3′-dideoxyinosine water solution

Stability and interaction with human serum albumin

Journal of Thermal Analysis and Calorimetry
Authors: Katarzyna Michalik, Zofia Drzazga, and Anna Michnik

Abstract  

A study of 2′,3′-dideoxyinosine (ddI) stability and its interaction with human serum albumin (HSA) was carried out by differential scanning microcalorimetry DSC. Scan rate dependent and irreversible endothermic thermal degradation of ddI was analyzed with use of kinetic approach. Observed process could be interpreted in terms of simple first-order one step kinetic model. Moreover it was shown that ddI bound weakly to the human serum albumin and stabilized this protein.

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