Authors:U. Bernhard, K. Kasperek, A. Höck, K. Vyska, C. Freundlieb, and L. Feinendegen
The concentrations of ten trace elements were assayed up to 3 hours after an infusion of an amino acid mixture. The changes
in metabolism induced by the administered amino acids led to characteristic alterations of the trace element concentrations.
These characteristic alterations could be explained by a mechanism which seems to compensate for membraneous charge alterations
caused by the metabolic reaction. Obviously, non essential trace elements are involved in this mechanism.
Authors:K. Wróblewski, A. Petruczynik, B. Buszewski, M. Szultka-Młyńska, H. Karakuła-Juchnowicz, and M. Waksmundzka-Hajnos
Vortioxetine is a new drug against major depressive disorder with high affinity for a range of different serotonergic targets in the central nervous system. Therapeutic drug monitoring is an important tool for the clinical management of patients receiving a pharmacotherapy, particularly in psychiatry. For this reason, determination of drug concentration in biological fluids is important for a rational dosage of drugs. Rapid and reliable analytical assays are also required to detect and identify drugs of toxicological importance. For analysis of vortioxetine by high-performance liquid chromatography (HPLC), no procedures for its determination in saliva have been reported and there are only a few ones for its determination in serum. A sensitive and selective highperformance liquid chromatography with diode array detector (HPLC-DAD) or mass spectrometer (HPLC-MS) method was developed for the fast quantification of vortioxetine in human saliva and serum. The determination was performed on a Synergi Polar RP column in isocratic mode under the optimal mobile phase containing 70% methanol, 20% acetate buffer at pH 3.5, 10% double distilled water, and 0.025 M L−1 diethylamine.
Kits containing macroaggregated albumin were prepared and compared with three commercial kits with respect to particle size distribution, radiochemical yield and biodistribution. Our preparation was comparable to the commercial products.
The objective of the present study was oriented to produce and purify polyclonal anti-PRL antibody as the main key store in
immunoradiometric assay (IRMA) using solid phase cellulose particles for determination of PRL in human sera. The preparation
of 125I-PRL was carried out by lactoperoxidase method for estimation of the titre of antibody production. The preparation of standards
was undertaken. The activation of cellulose particles using 1,1-carbonyl diimidazole (CDI) and coupling of these solid phase
particles with purified Rabbit anti-PRL were carried out. Optimization and validation of the assay were carried out. Results
revealed that the produced PRL polyclonal antibodies have high titre. Cellulose particles IRMA system was highly sensitive
and specific. The intra- and inter-assay variations were satisfactory. The recovery and dilution tests indicated accurate
calibration and appropriate matrix. The present technique agreed well with IRMA commercial kit. These cellulose particles
retained their characteristics during storage for 6 months at 4 °C. In conclusion, this low cost assay could be used as a
useful diagnostic tool for diagnosis and follow up of galactorrhea, infertility and pituitary adenoma.
Authors:A. Petruczynik, K. Wróblewski, and M. Waksmundzka-Hajnos
behavior. The effect of the concentration of IL or acetonitrile in mobile phases and effect of temperature was investigated and optimal eluent for analysis of psychotropic drugs in humanserum was chosen.
by Wako Pure Chemical Industries Ltd. Humanserum was purchased from MP Biomedicals Inc., and serum samples obtained from the patient were maintained at −20°C until required for analysis. MonoSpin C 18 and C 18 -CX were purchased from GL Science
Authors:Fatema Moni, Suriya Sharmin, Satyajit Roy Rony, Farhana Afroz, Shammi Akhter, and Md. Hossain Sohrab
, accurate, and precise method for determination of Esomeprazole in humanserum using HPLC suitable for Pharmacokinetic study, Bioavailability, and Bioequivalence study. Experimental Chemicals Esomeprazole and Pantoprazole (Internal standard, IS) were kindly
Authors:Liwei Cao, Lizhen Wu, Hailan Zhong, Hao Wu, Siyun Zhang, Jianxin Meng, and Fengyu Li
detection. Sample analysis In order to verify the feasibility of the established methods, the experiments were performed on spiked recovery tests in humanserum and urine. Fig. 5 and Fig. 6 show the chromatograms and electropherograms of serum and urine
Authors:Katarzyna Michalik, Zofia Drzazga, and Anna Michnik
A study of 2′,3′-dideoxyinosine (ddI) stability and its interaction with human serum albumin (HSA) was carried out by differential
scanning microcalorimetry DSC. Scan rate dependent and irreversible endothermic thermal degradation of ddI was analyzed with
use of kinetic approach. Observed process could be interpreted in terms of simple first-order one step kinetic model. Moreover
it was shown that ddI bound weakly to the human serum albumin and stabilized this protein.