Authors:Guido Wiesenberg, Martina Gocke, and Yakov Kuzyakov
Two powerful approaches are frequently used to trace incorporation and degradation of plant derived C in soil: 14C labelling/chasing and analysis of lipid composition. In this study, we coupled these approaches in order to trace short
term incorporation of plant derived lipids into rhizosphere and non-rhizosphere soil. Methodological optimization was required
and implied 14C liquid scintillation counting improvement for plant lipid extracts taking into account organic solvents, solvent-to-scintillation
cocktail ratio, and amount of lipids. Following method optimization, 14C data of fatty acids indicated a notable contribution of root derived lipids to rhizosphere and non-rhizosphere soil. Coupling
of 14C labelling/chasing with lipid analysis is a powerful and cheap approach for tracing of root derived C in soil allowing for
estimation of C budget, for determination of C formation and translocation within plants and from plant to soil, as well as
for identification of short term dynamics of specific compound classes within soil.
., Wong, S. T., Poser, S., Yano, S., Wayman, G., Deloulme, J. C., Chan, G., Storm, D. R. (1998) Cross talk between ERK and PKA is required for Ca 2+ stimulation of CREB-dependent transcription and ERK nuclear translocation. Neuron 21 , 869
Phenoloids with allelopathic effect (juglone, ctaechin, tannin, gallic acid t-cinnamic acid, caffeic acid, coumarin, thymol, salicin in 1mM concentration) cannot be detected after the absorption from the acceptor plant (bean) by the applied selective analytical method (TLC densitometry). Their localisation can be determined by histochemical reagens (ferrichloride, potassium bichromate, sodium hydroxide). In the foliage leaves and excised bean plants they are present mainly in the parenchymatuos elements of the vascular tissue already on the 3rd day, at the beginning of wilting. Some substances (tropanes) out of the studied allelopathic alkaloids (atropine, scopolamine, belladonnin, tropine and caffeine) can be detected only in small amounts (7-8%) or only in traces in the leaf. Others (e.g. caffeine) accumulate in substantial amount (almost 200%) in their original form. Alkaloids,as well as phenoloids, can be detected in lesf tissue by histochemical methods (Dragendorff and Meyer reagents).
Friebe, B., Hatchett, J. H., Gill, B. S., Sebesta, E. E. (1991): Transfer of hessian fly resistance from rye to wheat via radiation-induced terminal and intercalary chromosomal translocations.
Theor. Appl. Genet.
Authors:Ahmed Elfiky, Agnes Bonifacius, Joern Pezoldt, Maria Pasztoi, Paweena Chaoprasid, Pooja Sadana, Nagla El-Sherbeeny, Magda Hagras, Andrea Scrima, Petra Dersch, and Jochen Huehn
, effective translocation of Yops via the type III secretion system (T3SS) is only possible via direct cell–Yptb contact, established by different invasins (inv). Particularly, invasin A acts as a potent β 1 integrin ligand (mainly via α 4 β 1 integrin) [ 17