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in methanol. A working mix from the standard solution of 5 mg L −1 was prepared by transferring an appropriate aliquot of each stock solution into methanol. All stocks and working solutions were stored at −18 °C in the dark. 2.2 QuEChERS extraction

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Candan, F., Unlu, M., Tepe, B., Daferera, D., Polissiou, M., Sokmen, A. & Akpulat, H.A. (2003): Antioxidant and antimicrobial activity of the essential oil and methanol extracts of Achillea millefolium Afan. (Asteraceae). J. Ethnopharmacol. , 87

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. J. Chromatogr. 607 349 352 Naczk, M. & Shahidi, F. (1989): The effect of methanol

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the methanol extract of Cyclotrichium niveum . Pharm. Biol. , 47 , 219–229. Kizil M DNA damage protecting activity and in vitro antioxidant potential of the methanol extract of

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-Gutiérrez , A. (2010): Metabolite profiling and quantification of phenolic compounds in methanol extract of tomato fruit. Phytochemistry , 71 , 1848–1864. Fernández-Gutiérrez A. Metabolite

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standard in organic solvents (methanol for oxytetracycline and acetonitrile for erythromycin) to achieve a final concentration of 1 mg ml -1 . Stock solutions were stored at 4 °C for about 4 weeks. Working standards solutions of oxytetracycline (2.5100 ng g

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, extracted with methanol at 40 °C for 24 h, and then vacuum evaporated. Extraction yields of each extract are presented in Table 1 . 2.3 HPLC-DAD analysis Six different phenolic substances (chlorogenic acid, cyanidin, caffeic acid, trans -ferulic acid

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-Bidgoli, Z. & Batooli, H. (2009): GC/MS analysis and in vitro antioxidant activity of essential oil and methanol extracts of Thymus caramanicus Jalas and its main constituent carvacrol. Fd Chem. , 115 , 1524

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hydroxyflavones in absolute methanol. Part II. Ligands containing more than one chelating site. J. chem. Soc. (C) , 1309-1313. The aluminium(III) complexes of hydroxyflavones in absolute methanol. Part II. Ligands containing more than

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: Methanol versus water . Anal. Bioanal. Chem. , 388 , 1847 – 1857 . Puri , M. , Sharma , D. , Barrow , C.J. & Tiwary , A.K. ( 2012

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