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-tumor, anti-bacterial, and anti-aging effects etc. [ 1 – 3 ]. The major ingredients of Atractylodis are atractylenolide I and II that exhibited activities in anti-inflammatory and anticancer [ 4 – 6 ]. Therefore, the pharmacokinetics of atractylenolide I and

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letrozole in human plasma to evaluate the clinical efficacy and adverse reactions with clinical pharmacokinetic and therapeutic drug monitoring. In the present work, we described a LC-MS/MS method for the determination of letrozole in human plasma after one

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plasma and evaluate the pharmacokinetics of both drugs after an oral administration to their pharmaceutical preparation. Experimental Apparatus and Conditions Mass spectrometric analysis was performed using

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pharmacokinetic of letrozole in Wistar rat serum. The analytical method was a selective, with a linearity range of 0.15–100 μg/mL. LOQ was evaluated to be 0.15 μg/mL. In addition, there are different techniques developed, namely, capillary gas chromatography with

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Acta Chromatographica
Authors: H. Kalász, R. Laufer, P. Szegi, K. Kuca, K. Musilek, and K. Tekes

Summary

The pharmacokinetics of K-203, a recently synthesized bis-pyridinium aldoxime, have been investigated by reversed-phase HPLC. The K-203 content of serum samples was monitored by HPLC with ultraviolet absorbance detection at 286 nm. The low concentration of K-203 in samples of brain, eyes, and cerebrospinal fluid was also determined by HPLC; quantitative evaluation was possible by use of an amperometric detector.

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In many publications on the thin-layer chromatographic analysis of the medicinal plant extracts, striking skewness of the separated chromatographic bands with many phytochemicals can be seen, although no attention has ever been paid to this odd mass transfer effect. In our earlier studies on the thin-layer chromatographic enantioseparation of certain low molecular weight carboxylic acids, lateral relocation thereof (i.e., the side-wise deviation of their migration route from linearity) was registered. Then we found out that lateral relocation was observed with these analytes only, which structurally resembled molecular rotors. In this study, we investigate the role played by the thin-layer chromatographic stationary phases in lateral relocation of botanically relevant molecular rotors. Thus, three carboxylic acids were selected as the test analytes, all of them resembling molecular rotors and abundantly present in the medicinal plant extracts. We selected two most popular thin-layer chromatographic stationary phases also, i.e., silica gel (characterizing with microcrystalline chirality) and alumina (achiral). Lateral relocation of the test analytes was observed on the silica gel stationary phase only. A conclusion was drawn that the chiral stationary phase makes a complementary contribution to lateral relocation (along with the propeller-like molecular structure of the analytes). In our view, specially devised thin-layer chromatographic systems can prove a convenient nano-platform for future investigation of the drug transport patterns, advantageous in the pharmacokinetic studies.

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Summary

A sensitive validated high-performance liquid-chromatographic method for analysis of cilostazol in human plasma (in vitro) has been developed, and it was applied to determine pharmacokinetics of cilostazol in male albino rabbit. Cilostazol was extracted from human plasma (in vitro) by acetonitrile, and efficient chromatographic elution was achieved on a C18 column (250 × 4.60 mm i.d., 0.5 μm particle size) with an isocratic mobile phase [acetonitrile-50 mM acetate buffer (pH 5.0, glacial acetic acid)-water (50:20:30)] at flow rate of 1.5 mL min−1. Quantification was carried out by photo-diode array (PDA) detection at 248 nm. The linearity of the method was excellent over the range 0.2–2 μg mL−1 with low limits of detection (0.005 μg mL−1) and quantification (0.05 μg mL−1). The extraction recovery of the drug from plasma was consistently good (73.45–78.64%), with low relative standard deviation (0.44–1.65%). Robustness studies confirmed that peak area was unaffected by small changes in temperature, mobile phase (composition and pH). The maximum concentration (C max) in rabbit (in vivo) was determined 1.620 μg mL−1 at t max (0.51 h) with 0.63% RSD by validated bioanalytical method.

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A fast, simple, and sensitive reversed-phase high-performance liquid chromatography (RP-HPLC) method has been developed and fully validated for the determination of moxifloxacin (MXF) in rat plasma. MXF and gatifloxacin (internal standard, I.S.) were extracted from plasma by single-step protein precipitation with acidified acetonitrile. Chromatographic separation was accomplished in less than 8 min on an Atlantis ® T3 column with 0.4% aqueous triethylamine–methanol–acetonitrile (60:35:5, v/v/v) solution as mobile phase. Detection was achieved by fluorescence (λ excitation = 295 nm, λ emission = 500 nm), and the calibration curves were found to be linear over the plasma concentration range of 10–2,500 ng mL−1 with a mean correlation coefficient (r) of 0.9946 (n = 6). The intra- and inter-assay imprecision (% CV) was less than 2.4 and 3.3%, respectively, and the accuracy was >90%. The mean extraction recoveries for MXF and I.S. from plasma were 77 and 82%, respectively. The method was also validated for specificity, sensitivity, and stability; all the results were within the acceptable range. The proposed method was then successfully applied to the quantitative analysis of MXF in rat plasma samples, being a valuable and high-throughput assay to support ongoing pharmacokinetic studies on this promising anti-infective agent.

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, besides the combined effects, what influences would this combined application demonstrate on the active components in STVO and STAE as far as pharmacokinetics is concerned? To our knowledge, there have been some pharmacokinetic articles of luteoloside

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materials or pharmaceutical forms, the simultaneous determination and pharmacokinetics of these five isoflavones have not been reported, especially no method has been used to compare the pharmacokinetics parameters of DIFG in plasma. The gastric emptying

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