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and rapid ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method for the simultaneous determination of atractylenolide I, II, and III in rat plasma [ 1 ]. Plasma samples were processed by liquid–liquid extraction with
), respectively. 0.9% saline and sodium heparin were purchased from Biosharp (China). UPLC-grade methanol, and acetonitrile were purchased from Merck (Germany). Sutent 50 was from Virbac (France). Sodium carboxymethyl cellulose (CMC-Na) was purchased from Macklin
, the object of current study was to establish a sensitive UPLC–MS/MS method for quantitation of eupatilin in rat plasma for the first time and apply it in the investigation of the pharmacokinetic properties of eupatilin. Experimental
–tandem mass spectrometry (LC–MS/MS), ultra-performance liquid chromatography–MS/MS (UPLC–MS/MS) is more sensitive, possessing significant advantages in the investigation on pharmacokinetics of drugs. Meanwhile, the powerful separation and analysis capacity are
there is no literature about the determination of spiraeoside in biological samples by UPLC-MS/MS. In order to explore the therapeutic potential of spiraeoside in vivo, it is necessary to establish a rapid and simple bioanalytical method for its
coupled with tandem mass spectrometry (UPLC-MS/MS) was a powerful tool for characterizing unknown compounds in complex natural medicines and clarifying the multiple compound, multiple target pharmacological mechanisms [ 8–10 ], but there were some
behavior of shikonin, isobutyryl shikonin and β, βʹ -dimethylacryl alkanin in beagle dogs plasma. Ultra-high performance liquid chromatography (UPLC) is a commonly used chemical component separation technology, which has many advantages such as
determination of the Aconitum alkaloids from YGY in plasma for investigating its pharmacological and toxicological effects, as well as monitoring therapeutic efficacy in clinic. In this study, we established a sensitive UPLC–ESI–MS method for simultaneous
and rapid method based on ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) has been developed and validated for the determination of remimazolam and its major carboxylic acid metabolite (M1) in human urine [ 4 ]. The method
were extraction time, temperature, and percentage of methanol in distilled water, with preliminary tests based on the one-variable-at-a-time approach. Notably, the active component DPT was detected by ultra-performance liquid chromatography (UPLC
