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A study was conducted to evaluate the nephrotoxic effect of ochratoxin A (OTA) in broiler chickens. Forty Ross 308 broilers (6 days old) were divided into two groups: one group received daily, by gavage, ochratoxin A at a daily dose of 50 μg/kg body weight for up to 21 days, while the control group received only diluent (sunflower oil). After 21 days, the chickens were euthanised and the kidneys removed for analysis by histopathology and immunohistochemistry to detect an anti-apoptotic marker (Bcl-2), and by transmission electron microscopy. Macroscopically the kidneys were enlarged, showing degeneration and gout deposits. Histologically, glomerulonephrosis and tubulonephrosis were common lesions in all chicks. In two of the five chicks exposed to OTA for 21 days, focal tubular cell proliferation, multiple adenoma-like structures and Bcl-2-positive epithelial cells were identified in layers of the renal papilla and in convoluted tubules. Transmission electron microscopy of the proximal convoluted tubules identified abnormal forms of mitochondria. The nephrotoxic effect of ochratoxicosis in chickens is probably due to carcinogenic changes induced in the epithelial tissues.

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The linear hypertrophic scar has become the most common type of pathologic scarring. Silicone-gel sheeting is the first line therapy while intralesional steroid is the second. A light and electron microscopic analysis was carried out to reveal differences in tissue reaction following the two different treatments.Two groups of 12 patients each were treated for 4 months. For the first group, diluted Triamcinolone acetonide was injected until an inactive state was achieved. The other group of patients was treated with silicone-gel sheeting. The scars were examined every two weeks and their appearance documented. After reaching the expected therapeutic response, inactive scars were removed. The excised scars were evaluated through light microscopic histopathology and electron microscopy.The light and electron microscopic observations revealed marked differences following treatments. The activity of fibroblasts and the numbers of collagen fibers forming bundles decreased and the orientation of the collagen fibers was more variable in the treated scars. The amount of elastic fibers increased after both steroid and silicone-gel sheeting treatment. Vascularization was also slightly changed, with more capillaries and fewer pre-capillary arteries detected in the treated scars. Both treatments resulted in the same decrease in score but steroid treatment was more rapid in onset. We suggest that the two different treatments work through different mechanisms, although the final functional outcome is similar.

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Acta Veterinaria Hungarica
Authors:
Viktor Molnár
,
Máté Jánoska
,
Balázs Harrach
,
Róbert Glávits
,
Nimród Pálmai
,
Dóra Rigó
,
Endre Sós
, and
Mátyás Liptovszky

This paper describes the detection of a novel herpesvirus in a Serotine bat ( Eptesicus serotinus ) in Hungary. The rescued animal showed signs of icterus and anorexia and died within a day, in spite of immediate supportive therapy. Autopsy confirmed the clinical picture by the major lesions observed in the liver. Histopathology revealed vacuolar degeneration in the hepatocytes and leukocytosis in the sinusoidal lumina. By electron microscopy, hydropic degeneration and apoptotic cells with a pycnotic nucleus were found in the liver. Bacteriological examinations gave negative results. As part of a routine screening project, detection of adeno-and herpesviruses from homogenised samples of the liver, lungs and small intestines was attempted by nested polymerase chain reaction (PCR) assays. The adenovirus PCR ended with negative results. The herpesvirus PCR resulted in an amplification product of specific size. The nucleotide sequence of the amplicon was determined and analysed by homology search and phylogenetic analysis. A novel herpesvirus was identified, which seemed to be most closely related to members of the genus Rhadinovirus within the subfamily Gammaherpesvirinae . The causative role of the detected rhadinovirus in the fatal condition of the Serotine bat could not be proven, but it is most likely that reactivation from a latent infection allowed the detection of the virus by PCR.

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Abstract

Enterocolitis caused by Campylobacter jejuni represents an important socioeconomic burden worldwide. The host-specific intestinal microbiota is essential for maintaining colonization resistance (CR) against C. jejuni in conventional mice. Notably, CR is abrogated by shifts of the intestinal microbiota towards overgrowth with commensal E. coli during acute ileitis. Thus, we investigated whether oral transplantation (TX) of ileal microbiota derived from C. jejuni susceptible mice with acute ileitis overcomes CR of healthy conventional animals. Four days following ileitis microbiota TX or ileitis induction and right before C. jejuni infection, mice displayed comparable loads of main intestinal bacterial groups as shown by culture. Eight days following ileitis induction, but not ileal microbiota TX, however, C. jejuni could readily colonize the gastrointestinal tract of conventional mice and also translocate to extra-intestinal tissue sites such as mesenteric lymph nodes, spleen, liver, and blood within 4 days following oral infection. Of note, C. jejuni did not further deteriorate histopathology following ileitis induction. Lack of C. jejuni colonization in TX mice was accompanied by a decrease of commensal E. coli loads in the feces 4 days following C. jejuni infection. In summary, oral ileal microbiota TX from susceptible donors is not sufficient to abrogate murine CR against C. jejuni.

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The endocytotic c-type lectin receptor DEC-205 is highly expressed on immature dendritic cells. In previous studies, it was shown that antigen-targeting to DEC-205 is a useful tool for the induction of antigen-specific Foxp3+ regulatory T cells and thereby can prevent inflammatory processes. However, whether this approach is sufficient to mediate tolerance in mucosal tissues like the gut is unknown. In this study, we established a new mouse model in which the adoptive transfer of naive hemagglutinin (HA)-specific CD4+Foxp3 T cells into VILLIN-HA transgenic mice leads to severe colitis. To analyze if antigen-targeting to DEC-205 could protect against inflammation of the gut, VILLIN-HA transgenic mice were injected with an antibody–antigen complex consisting of the immunogenic HA110–120 peptide coupled to an α-DEC-205 antibody (DEC-HA) before adoptive T cell transfer. DEC-HAtreated mice showed significantly less signs of intestinal inflammation as was demonstrated by reduced loss of body weight and histopathology in the gut. Strikingly, abrogated intestinal inflammation was mediated via the conversion of naive HA-specific CD4+Foxp3 T cells into HA-specific CD4+Foxp3+ regulatory T cells. In this study, we provide evidence that antigen-targeting to DEC-205 can be utilized for the induction of tolerance in mucosal organs that are confronted with large numbers of exogenous antigens.

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Acta Veterinaria Hungarica
Authors:
Salvatore Parrillo
,
Ippolito De Amicis
,
Roberta Bucci
,
Jasmine Hattab
,
Domenico Robbe
, and
Augusto Carluccio

Abstract

Placental abnormalities more frequently occur during pregnancy of somatic cell clones and may lead to pregnancy loss or dystocia. Adventitious placentation, or diffuse semi-placenta, is determined by the development of areas of accessory placentation between the cotyledons due to the abnormal growth of placentomes.

After a full-term pregnancy, a 3-year-old Jersey heifer was referred for dystocia which resulted in the delivery of a dead calf. The cause of dystocia was found to be foetal malposition, while the placenta was physiologically expelled after dystocia resolution.

Grossly, cotyledons appeared reduced in size and number in one placental horn, while the surface of the other horn was covered with microplacentomes. Numerous villous structures without trophoblastic coating were highlighted after histopathology. The dominant sign was an inflammatory reaction. The findings were consistent with inter-cotyledonal placentitis, which led to adventitial placentation.

Diffuse semi-placenta compensates for the inadequate development of placentomes and may occur as a congenital or acquired defect. The outcome depends on its severity: in the worst scenario, pregnancy may not proceed beyond midterm and may be complicated by hydrallantois. In the case under examination, the dimensions of the cotyledons (from 2 to 10 cm) allowed for the natural course of pregnancy.

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Orvosi Hetilap
Authors:
Gábor Füredi
,
Anna Szilágyi
,
Zsuzsa Bencsik
, and
Áron Altorjay

A mellékvese adenomatoid tumora igen ritka, benignus, aszimptomatikus, általában véletlenül felfedezett elváltozás. A szerzők esetismertetésükben egy 32 éves férfi kórtörténetét mutatják be, akit láz és deréktáji fájdalmak miatt vizsgáltak. A kivizsgálás részét képező hasi UH-, majd az ezt követő hasi CT-vizsgálat igazolta a jobb mellékvese térfoglaló elváltozását. A betegnél adrenalectomiát végeztek, a szövettani vizsgálat a jobb mellékvese adenomatoid tumorát véleményezte. Az eset kapcsán áttekintik – az irodalmi adatok alapján – az adenomatoid tumorok előfordulási gyakoriságát, tünettanát, a diagnosztika-differenciáldiagnosztika buktatóit, a kezelési lehetőségeket és a prognózist.

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Az elasztikus rostfestés jelentősége a colorectalis carcinomák vascularis invázióinak kimutatása szempontjából

The importance of elastic staining in detecting vascular invasion in colorectal carcinoma

Magyar Onkológia
Authors:
Barna Bogner
and
Géza Hegedűs

2006 Routine elastic staining assists detection of vascular invasion in colorectal cancer Histopathology 49 487 492 . 2

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Hypermucinosus és kehelysejtszegény, gyulladásos bélbetegséghez társult, non-conventionalis dysplasia colorectalis adenocarcinoma mellett

Hypermucinous and goblet cell-deficient, IBD-associated, non-conventional dysplasia besides colorectal adenocarcinoma

Orvosi Hetilap
Authors:
Szintia Almási
,
Bence Baráth
,
Panna Szaszák
,
Bence Kővári
, and
Anita Sejben

-convetional dysplasia is frequently associated with low-grade tubuloglandular and mucinous adenocarcinomas in inflammatory bowel disease. Histopathology 2023; 83: 276

Open access
Orvosi Hetilap
Authors:
Miklós Horváth
,
Mária Garamszegi
,
Barna Bogner
, and
Tamás Beró

412 Cook, D. S., Williams, G. T.: Duodenal pseudolipomatosis. Histopathology, 1998, 33 , 394–395. Williams G. T

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