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potential antibacterial properties of methanol extracts from fungus Ganoderma applanatum . Chemotherapy , 52 , 241–244. Ghodsa S. Investigation of potential antibacterial properties of

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Progress in Agricultural Engineering Sciences
Authors: Szilvia Bánvölgyi, Eszter Dusza, Fiina K. Namukwambi, István Kiss, Éva Stefanovits-Bányai, and Gyula Vatai

–Ciocalteu reagent was purchased from Merck KGaA. (Darmstadt, Germany), the gallic acid was obtained from Sigma-Aldrich, Chemicals Company (St. Louis, MO, USA). The methanol and sodium carbonate were purchased from Reanal Laborvegyszer Ltd. (Budapest, Hungary

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consisted of methanol and 1.5% acetic acid (45:55 v/v) and was injected at a flow rate of 0.5 mL min −1 at 25 °C. Absorption was detected at 320 nm using the Shimadzu LC-2010 ultraviolet detector. Each sample was injected in a 10 µL volume. The FA content

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protein digestibility. 2.3 Bioactive components Antioxidant activity (AOA) in terms of DPPH radical scavenging activity was measured by the method of Gujral et al. (2012) using acidified methanol extract and measuring the absorbance at 515 nm

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solution in ethanol were obtained from Merck (Budapest, Hungary). Methanol, sodium hydroxide, and anhydrous sodium sulphate were obtained from Reanal Laborvegyszer (Budapest, Hungary). 1,2-Bis-palmitoyl-3-chloropropanediol-d 5 internal standard was

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Acta Alimentaria
Authors: C. Cano-Molina, A. López-Fernández, N. Díaz-González, R. González-Barrio, N. Baenas, M.J. Periago, and F.J. García-Alonso

methanol/tetrahydrofuran (v/v, 50:50) containing 0.1% butylated hydroxytoluene ( Seybold et al., 2004 ). Chromatography separation was performed using a C30 column 250 × 4.6 mm, 5 µm i.d. (Análisis Vínicos S.L., Villarrobledo, Spain) in an Agilent HPLC

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saline (PBS) pH=7.4, chloroform and methanol (Merck, Germany) were purchased. 1.2 Preparation of extracts and encapsulation of the powder of extracts The extracts were prepared using dried (for more precise and easier extraction), ground berries of two

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was prepared by dissolving 100 mg of coumarin standard (Serva, Germany) in 80% methanol and adjusting the final volume to 100 ml (1.0 mg ml -1 ). Seven dilutions of coumarin with concentrations ranging from 0.005-0.5000 mg ml -1 were prepared and

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h. Wells were then washed three times with sterile phosphate-buffered saline (PBS) and fixed with 200 µL of 99% methanol (Merck) for 15 min. Plates were stained with 0.1% crystal violet for 15 min. The stained biofilm was solubilised with 160 µL of

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differences might be ascribed to differences in the chemical composition of these extracts and in the sensitivity of the tested bacteria. Table 2. Antibacterial activity of methanol extracts of the immature and mature faba bean seeds Vicia faba seeds Zone of

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