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concentrations were determined by an enzyme-linked immunosorbent assay (ELISA) method using canine-specific commercial kits (MyBioSource, Inc., San Diego, CA, USA). All plasma samples were analysed twice according to the manufacturer’s recommendations. Both intra
) was purchased from Beijing Solarbio Science & Technology Co., Ltd (Beijing, China). The activity of myeloperoxidase (MPO) was analyzed by the MPO Activity assay kit (Cat no:ab105136, Abcam). The Mouse TNF-α ELISA kit (Cat No: SEKM-0034) and the
colostrum. When colostrum is analysed by direct methods such as RID and ELISA, the lamb's ability to absorb immunoglobulins will likely be lost by the time the results are produced ( Zarrilli et al., 2003 ; Aydoğdu et al., 2015 ). Therefore, diagnostic
measured with a competitive ELISA protocol based on the biotin-streptavidin-peroxidase system. ELISA assay plates coated with a primary antibody (polyclonal antibody anti-ANG-II, RAB 002-12, Phoenix Pharmaceuticals, Inc., Belmount, CA, USA), secondary
VF, ADP, CHEM and CIT levels The VF, ADP, CHEM and CIT levels were measured in blood serum samples according to the manufacturer’s instructions using the ELISA technique. Absorbance values were recorded at a wavelength of 450 nm. Serum
software (Treestar, SanCarlos, CA). Enzyme-linked immunosorbent assay (ELISA) To extract the intestinal mucus the fat and connective tissue of the serosa were removed using forceps and scissors, and the intestine was transferred into a petri dish. The
against sonicated WCL of the S. dysenteriae type 4 strain using ELISA [ 12 ]. Wells were coated with S. dysenteriae type 4 WCL (1 µg/well) and incubated overnight at 4 °C. Serum IgG and IgA antibody titers were determined by using peroxidase
formazan crystals were measured at 550 nm with an ELISA reader (Anthos Labtech Elisa Reader, Hungary). The antibacterial effect of the antiseptics was compared with untreated control Ti discs with the developed biofilm which were only rinsed with sterile 1
sampling and EGS ELISA measurements (Animal Health Trust Contract Research Group Laboratory, Newmarket, UK) as follows. The first vaccine was applied in June 2015 after the first vaccination treatment (T0). Thirty days (T30), 44 days (T44) and 210 days (T
. Oestradiol and progesterone hormone levels were determined by competitive ELISA using kits Human/Monkey/Mouse E2 (Estradiol ELISA Kit (E-EL0150 96T) and Pg (Progesterone) ELISA Kit (E-EL-0154 96T) according to the manufacturer's (Elabscience) manual
