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, no other obvious signs of disease or mortality were observed among the 2-year-old wels catfish. One fish with the most prominent rash was used for PCR testing for a possible viral origin of the skin disease. A pea-sized piece from the damaged skin

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Microchimaerismus kimutatása nagy érzékenységű droplet digitális PCR technikával és szerepe a vérképzőőssejt-transzplantált betegek monitorozásában

Detection of microchimerism with high sensitivity droplet digital PCR technique and its significance in monitoring of hematopoietic stem cell transplanted patients

Orvosi Hetilap
Authors:
Zoltán Őrfi
,
Nóra Meggyesi
,
Lívia Varga
,
András Bors
,
László Gopcsa
,
Melinda Paksi
,
Viktor Lakatos
,
Krisztián Kállay
,
Gergely Kriván
,
Alexa Jónás
,
Attila Tordai
,
István Vályi-Nagy
,
Péter Reményi
, and
Hajnalka Andrikovics

] 16 Kliman D, Castellano-Gonzalez G, Withers B, et al. Ultra-sensitive droplet digital PCR for the assessment of microchimerism in cellular therapies. Biol Blood Marrow Transplant. 2018; 24

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Shi, L., Ho, J., Norling, L. A., Roy, M., Xu, Y., (1999) A real time quantitative PCR-based method for the detection and quantification of simian virus 40, Biologicals 27 , 241-252. A real time quantitative PCR-based method

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Acta Biologica Hungarica
Authors:
J. Saju
,
Sz. Németh
,
Réka Szűcs
,
Rashmi Sukumaran
,
Z. Lim
,
L. Wong
,
L. Orbán
, and
M. Bercsényi

Welsh, J., McClelland, M. (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18 , 7213–7218. McClelland M. Fingerprinting genomes using PCR with

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sensitivity of the culture method [ 6 ]. Furthermore, the use of PCR for the detection of bacterial DNA from CSF is now rapidly becoming widespread, and employing this assay can significantly increase the speed and accuracy of identification of the pathogen

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Békefi, Z., Tobutt, K. R., Sonneveld, T. (2003): Determination of (in)compatibility genotypes of Hungarian sweet cherry ( Prunus avium L.) accessions by PCR based methods. Int. J. Hort. Sci. , 9 , 37

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It is well established that the ingestion of cereal prolamins, such as gluten, causes the characteristic symptoms of celiac disease (CD) in people predisposed to it. DNA-based PCR method provides new ways to detect gluten in processed foodstuffs, such as bread. The aim of this work was to adapt a new primer pair combination and to initiate a carefully elaborated PCR methodology to experiment with DNA-based analysis. At first, the purity of cleaned DNA was verified using B49317 and A49855 chloroplast DNA primer pair. Then TR01/2 wheat specific PCR primer pair was used for checking the origin of the DNA, and P1/2 microsatellite (SSR) adapted primer pair for detecting allergen (gluten) specific residues. Method optimisation was achieved with cereal flour samples, then bread and dry pasta products from wheat were used, which were analysed as heat-treated samples with three primer pairs. The gluten specific primer pair was tested on cross-reactive cereals such as rye, barley, triticale and on some questionable cereals, such as oat, and pseudo-cereals, e.g. buck wheat and amaranth.

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of North America and development of a differential PCR-RFLP assay to detect and differentiate infections between PCV-1 and PCV-2. J. Clin. Microbiol. 38, 2494--2503. Genetic characterization of type-2 porcine circovirus (PCV

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Környezetből származó mikrobiális nukleinsavminták vizsgálati lehetőségei

Examination possibilities of microbial nucleic acid samples derived from the environment

Orvosi Hetilap
Authors:
Ivett Kriszta Kerekes
,
Ádám Nagy
,
Ágnes Ősz
, and
Péter Zalka

metagenomics: from microbiology to DNA sequencing technologies and bioinformatics. Front Genet. 2015; 6: 348. 8 Jeong J, Mun S, Oh Y, et al. A qRT-PCR method capable of

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Acta Veterinaria Hungarica
Authors:
Urška Henigman
,
Majda Biasizzo
,
Stanka Vadnjal
,
Andrej Kirbiš
,
Ivan Toplak
, and
Darja Barlič-Maganja

The aim of this study was to determine the prevalence of Vibrio parahaemolyticus in shellfish samples harvested along the Slovenian coast. Shellfish samples of Mediterranean mussels (Mytilus galloprovincialis) were collected along the Slovenian coast at four locations (Seča, Piran, Strunjan and Debeli Rtič) between 2006 and 2008. Samples were examined and analysed for the presence of V. parahaemolyticus by conventional and molecular methods. The presence of Vibrio in the samples was examined by conventional methods on plate grown bacterial cells before and after enrichment in alkaline saline peptone water (ASPW). PCR methods were used for the detection of V. parahaemolyticus-specific toxR and tlh genes and of the virulence-associated tdh and trh genes. Out of 168 samples examined, 24 were positive for toxR and tlh genes by PCR from enrichment broth. Five out of 62 (8.1%), 4 out of 32 (12.5%) and 15 out of 74 (20.2%) samples were positive in 2006, 2007 and 2008, respectively. Colonies of V. parahaemolyticus were isolated from only one sample positive for V. parahaemolyticus by PCR.

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