Authors:L.V. Shchukina, T.A. Pshenichnikova, E.K. Khlestkina, S. Misheva, T. Kartseva, A. Abugalieva, and A. Börner
Bread wheat is the primary bread crop in the majority of countries in the world. The most important product that is manufactured from its grain and flour is yeast bread. In order to obtain an excellent bread, grain with high physical properties is needed for flour and dough. The Russian spring wheat cultivar Saratovskaya 29 is characterized by its exclusively high physical properties of flour and dough. The purpose of this work was to identify the chromosomes carrying the main loci for these traits in Saratovskaya 29 and to map them using recombinant substitution lines genotyped with molecular markers. A set of inter-varietal substitution lines Saratovskaya 29 (Yanetzkis Probat) was used to identify the “critical” chromosomes. The donor of individual chromosomes is a spring cultivar with average dough strength and tenacity. Substitution of 1D and 4D*7A chromosomes in the genetic background of Saratovskaya 29 resulted in a significant decrease in the physical properties of the dough. Such a deterioration in the case of 1D chromosome might be related to the variability of gluten protein composition. With the help of recombinant substitution double haploid lines obtained from a Saratovskaya 29 (Yanetzkis Probat 4D*7A) substitution line the region on the 4D chromosome was revealed in the strong-flour cultivar Saratovskaya 29, with the microsatellite locus Xgwm0165 to be associated with the unique physical properties of flour and dough. The detected locus is not related to the composition gluten proteins. These locus may be recommended to breeders for the selection of strong-flour cultivars. Additionally, a QTL associated with vitreousness of grain was mapped in the short arm of chromosome 7A.
Authors:F. Yilmaz Korkmaz, N.B. Tuncel, M. Özer, and N. Yilmaz Tuncel
, and therefore procurement of immature rice grains as raw material is easy and cheap. However, it should be emphasized that IRGF should be stabilised before use for food safety reasons. Moreover, due to being gluten-free, IRGF is also a promising
Authors:M. Studnicki, M. Wijata, G. Sobczyński, S. Samborski, and J. Rozbicki
Eleven spring wheat cultivars were compared in terms of the stability of their grain yield and grain quality. The cultivars’ stability was evaluated separately at two different crop management levels – moderate-input management and high-input management. Three stability models were used for the two crop management levels based on a linear mixed model framework with restricted maximum likelihood. The Shukla model was the most appropriate for the evaluation of stability of tested spring wheat cultivars. The thousand-grain weight, starch content, Zeleny sedimentation value and test weight were characterized, and the stability ranking cultivars at moderate-input management level was mostly consistent with the rank of cultivars 24 for high-input management level. For grain yield, grain protein content and wet gluten content, the stability rankings were not consistent. Cultivars ‘Monsun’ and ‘Parabola’ are the most stable cultivars for grain yield in moderate-input management and high-input management, respectively. Cultivar ‘Hewilla’ was the stable cultivar for all quality traits at moderate-input management. Cultivar ‘Arabella’ was the most stable cultivar at high-input management level.
Authors:J. P. Zhou, Y. Cheng, L. L. Zang, E. N. Yang, C. Liu, X. L. Zheng, K. J. Deng, Y. Q. Zhu, and Y. Zhang
In this study, a new substitution line, 12-5-1, with 42 chromosomes that was derived from BC3F2 descendants of the hybridization between Triticum aestivum cv. CN19 and Aegilops biuncialis was created and reported. The 12-5-1 was immune to both powdery mildew and stripe rust and has stable fertility. Multi-color fluorescence in situ hybridization indicated that 12-5-1 was a substitution line 1Mb(1B). The seed storage protein electrophoresis showed that 12-5-1 presented high molecular weight glutenin subunits (2 + 12) of CN19 and a new subunit designated as M which apparently originated from parent Ae. biuncialis, and absent 7 + 8 subunits. Additionally, the flour quality parameters showed that the protein content, Zeleny sedimentation value, wet gluten content, and grain hardness and mixing time of 12-5-1 were signifiantly higher than those of its parent CN19. Moreover, 5 pairs of the chromosome 1Mb-specifi polymerase chain reaction-based landmark unique gene markers, TNAC1021, TNAC1026, TNAC1041, TNAC1-02 and TNAC1-04, were also obtained. The new substitution line 1Mb(1B) 12-5-1 could be a valuable source for wheat improvement, especially for wheat end product quality and resistance to disease.
Authors:F. Hailu, M. Labuschagne, A. Van Biljon, H. Persson Hovmalm, and E. Johansson
Quality of durum wheat is of importance for pasta production. Our aim was to evaluate the quality of released durum wheat at Ethiopia by the use of pheno-quality traits as well as composition and amount and size distribution of proteins; these attributes were also compared with the same ones for Spanish released wheat grown in the same environment. Large variation was obtained among the released durum wheat for all parameters investigated. The evaluation of protein parameters indicated the varieties Klinto, LD-357, Tob-66 and Ude to have suitable protein composition for pasta production, while Tob-66 showed high protein concentration and Tob-66, LD-357 and Yerer showed high gluten strength. The most promising Ethiopian variety for pasta production as to the results from the present study was Tob-66. Also, LD-357 showed promising protein characteristics, although the kernels were white, which is not desired for pasta production. Some of the evaluated varieties might be of interest for production of local leavened bread although the quality is not good enough for pasta production.
Authors:Y. Liu, S. Wang, C. Wang, G. Chen, H. Cao, Y. Wang, W. Ma, Y. Hu, and Y. Yan
A comparative proteomic analysis of grain proteins during five grain developmental stages of wheat cultivar Chinese Spring (CS) and its 1Sl/1B substitution line CS-1Sl(1B) was carried out in the current study. A total of 78 differentially expressed protein (DEP) spots with at least 2-fold expression difference were detected by two-dimensional electrophoresis (2-DE). Among these, 73 protein spots representing 55 differentially expressed proteins (DEPs) were successfully identified by matrix-assisted laser desorption/ionization time-offlight tandem mass spectrometry (MALDI-TOF/TOF-MS). Differential protein spots between the two genotypes were analyzed by cluster software, which revealed significant proteome differences. There were 39 common spots (including 33 DEPs) that showed significant difference between the two lines across five grain developmental stages, of which 14 DEP spots (including 11 DEPs) were mainly involved in carbohydrate metabolism that were encoded by the genes on 1B chromosome while 25 DEP spots (including 12 DEPs) were mainly related to stress response and gluten quality that were encoded by 1S1 chromosome. These results indicated that the Sl genome harbors more stress and quality related genes that are potential valuable for improving wheat stress resistance and product quality.
Authors:I. Bellil, O. Hamdi, A. Benbelkacem, and D. Khelifi
Wheat endosperm storage proteins are the major components of gluten. They play an important role in dough properties and in bread making quality in various wheat varieties. In the present study, the different alleles encoded at the 5 glutenin loci were identified from a set of 38 tetraploid wheat germplasm obtained from interspecific crosses between durum wheats (Triticum turgidum L. ssp. durum (Desf.) Husn.) and their relatives (T. dicoccum Schübl. and T. polonicum L.) using SDS-PAGE. At Glu-A1 and Glu-B1, encoding high molecular weight glutenin subunits (HMW-GS), 2 and 4 alleles were observed, respectively. Low molecular weight glutenin subunits (LMW-GS) displayed similar polymorphism, as 3, 5 and 3 alleles were identified at loci Glu-A3, Glu-B3 and Glu-B2, respectively. One new allele was detected at Glu-B3 locus and appeared in nine accessions obtained from five crosses. This allele codes for five subunits (2 + 8 + 9 + 13 + 18), encoded by the Glu-B3b without subunit 16 plus subunits 2 and 18. A total of 38 patterns resulted from the genetic combination of the alleles encoding at the five glutenin loci. This led to a significantly higher Nei coefficient of genetic variation in Glu-1, Glu-3 and Glu-B2 loci (0.54). The germplasm analyzed exhibited allelic variation in HMW and LMW glutenin subunit composition and the variation differed from that of tetraploid wheats of other countries. The presence of high quality alleles in glutenin loci have led the accessions to be considered as an asset in breeding programs aimed for wheat quality.
Authors:K. Tremmel-Bede, P. Mikó, M. Megyeri, G. Kovács, S. Howlett, B. Pearce, M. Wolfe, F. Löschenberger, B. Lorentz, L. Láng, Z. Bedő, and M. Rakszegi
Six cropping populations, three variety mixtures and one diversity population were developed from winter wheat varieties and studied for physical, compositional and end-use quality traits for three years (2011–2013) under different European climatic and management conditions in order to study the stability of these traits resulted by the genetic diversity. The beneficial compositional and nutritional properties of the populations were assessed, while variation and stability of the traits were analysed statistically. No significant differences were found among the populations in low-input and organic management farming systems in the physical, compositional and processing properties, but there was a difference in the stability of these traits. Most of the populations showed higher stability than the control wheat variety, and populations developed earlier had higher stability than those developed later. Furthermore, some populations were found to be especially unstable for some traits at certain sites (mostly at Austrian, Swiss and UK organic sites). Protein content of the populations was high (13.0–14.7%) without significant difference among them, but there was significant variation in their gluten content (28–36%) and arabinoxylan content (14.6–20.3 mg/g). The most outstanding population for both protein and arabinoxylan content was a Hungarian cropping population named ELIT-CCP. It was concluded that the diversity found in the mixtures and CCPs have stabilizing effect on the quality parameters, but a higher stability was observed under low-input than under organic conditions. These results could be beneficial not only for breeders but also for the consumers in the long run.