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confirm the specificity of the amplification reactions, a melting curve was recorded. Each sample was replicated three times; the value of the threshold cycle (Ct) was the same as that of the corresponding mean. The relative fold expression of each mRNA

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, 6, 7, and 8, respectively. The increase amount of T m is comparable to that observed for classical intercalators [ 33 – 35 ]. Fig. 4 Melting curves of CT DNA in the absence and presence of 1

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Journal of Behavioral Addictions
Authors: Mitra-Sadat Sadat-Shirazi, Nasim Vousooghi, Bentolhoda Alizadeh, Seyed Mohammad Makki, Seyed Zeinolabedin Zarei, Shahrzad Nazari, and Mohammad Reza Zarrindast

Biosystems, USA). For each gene, the real-time PCR reaction was adjusted in such a way that a single product was amplified for each primer pair. The optimized annealing temperature of reactions was from 60 to 63 °C. Observing a single peak in melting curve

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European Journal of Microbiology and Immunology
Authors: Hans Kollenda, Ralf Matthias Hagen, Miriam Hanke, Sandra Rojak, Rebecca Hinz, Lars Wassill, Sven Poppert, Egbert Tannich, and Hagen Frickmann

with DNA filter paper, loop-mediated isothermal amplification, and melting curve analysis . Jpn J Infect Dis . 2009 ; 62 : 20 – 5 . 40. Singh R , Savargaonkar D , Bhatt R , Valecha N . Rapid detection of Plasmodium vivax in saliva and blood

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