Authors:Mahrokh Samadi, Alireza Shirpoor, Ali Taghizadeh Afshari, Fatemeh Kheradmand, Yousef Rasmi, and Maryam Sadeghzadeh
confirm the specificity of the amplification reactions, a meltingcurve was recorded. Each sample was replicated three times; the value of the threshold cycle (Ct) was the same as that of the corresponding mean. The relative fold expression of each mRNA
Authors:Mitra-Sadat Sadat-Shirazi, Nasim Vousooghi, Bentolhoda Alizadeh, Seyed Mohammad Makki, Seyed Zeinolabedin Zarei, Shahrzad Nazari, and Mohammad Reza Zarrindast
Biosystems, USA). For each gene, the real-time PCR reaction was adjusted in such a way that a single product was amplified for each primer pair. The optimized annealing temperature of reactions was from 60 to 63 °C. Observing a single peak in meltingcurve
Authors:Hans Kollenda, Ralf Matthias Hagen, Miriam Hanke, Sandra Rojak, Rebecca Hinz, Lars Wassill, Sven Poppert, Egbert Tannich, and Hagen Frickmann
with DNA filter paper, loop-mediated isothermal amplification, and meltingcurve analysis . Jpn J Infect Dis . 2009 ; 62 : 20 – 5 .
40. Singh R , Savargaonkar D , Bhatt R , Valecha N . Rapid detection of Plasmodium vivax in saliva and blood