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was prepared by dissolving 100 mg of coumarin standard (Serva, Germany) in 80% methanol and adjusting the final volume to 100 ml (1.0 mg ml -1 ). Seven dilutions of coumarin with concentrations ranging from 0.005-0.5000 mg ml -1 were prepared and

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h. Wells were then washed three times with sterile phosphate-buffered saline (PBS) and fixed with 200 µL of 99% methanol (Merck) for 15 min. Plates were stained with 0.1% crystal violet for 15 min. The stained biofilm was solubilised with 160 µL of

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Acta Alimentaria
Authors: C. Cano-Molina, A. López-Fernández, N. Díaz-González, R. González-Barrio, N. Baenas, M.J. Periago, and F.J. García-Alonso

methanol/tetrahydrofuran (v/v, 50:50) containing 0.1% butylated hydroxytoluene ( Seybold et al., 2004 ). Chromatography separation was performed using a C30 column 250 × 4.6 mm, 5 µm i.d. (Análisis Vínicos S.L., Villarrobledo, Spain) in an Agilent HPLC

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% methanol with a flow rate of 1 mL min −1 and column temperature of 25 °C, and UV– Visible detector fixed at 260 nm. 2.6 Statistical analysis Analysis of variance (ANOVA) was applied and comparison was made through Tukey's test with the least significant

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Acta Alimentaria
Authors: Cs. Dobolyi, K. Inotai, I. Bata-Vidács, D. Sárkány, O. Csernus, S. Kocsubé, B. Tóth, A. Szekeres, and J. Kukolya

2 mL dichloromethane. The extracts were stored at −20 °C until analysis. To each culture flasks containing corn grit samples 20 mL methanol was added and the mixtures were transferred into a Stomacher bag. The samples were homogenised for 45 s in a

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dissolved in isooctane, and ester derivative was prepared with 2M KOH in methanol and neutralized with NaHSO 4 . The analysis of fatty acid methyl-esters were performed by GC-MS (Shimadzu, 2010; Supleco-wax column). FAME-mix and Grain-mix standards were used

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