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differences might be ascribed to differences in the chemical composition of these extracts and in the sensitivity of the tested bacteria. Table 2. Antibacterial activity of methanol extracts of the immature and mature faba bean seeds Vicia faba seeds Zone of

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citrate, chloroform, acetone, toluene, acetic acid, potassium hydroxide, trichloroacetic acid, methanol, acetonitrile, and tetrahydrofuran were purchased from Kelon Chemical Reagent Factory, Chengdu, China. The standards of glutamic acid (Glu), aspartic

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Acta Alimentaria
Authors: C. Cano-Molina, A. López-Fernández, N. Díaz-González, R. González-Barrio, N. Baenas, M.J. Periago, and F.J. García-Alonso

methanol/tetrahydrofuran (v/v, 50:50) containing 0.1% butylated hydroxytoluene ( Seybold et al., 2004 ). Chromatography separation was performed using a C30 column 250 × 4.6 mm, 5 µm i.d. (Análisis Vínicos S.L., Villarrobledo, Spain) in an Agilent HPLC

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Frozen and dehydrofrozen quince sample extracts were obtained by methanol extraction and stored at 4 °C ( Hajji et al., 2020 ). Total polyphenol content (TPC) was determined by the Folin–Ciocalteu method using gallic acid as the reference compound, and

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% methanol with a flow rate of 1 mL min −1 and column temperature of 25 °C, and UV– Visible detector fixed at 260 nm. 2.6 Statistical analysis Analysis of variance (ANOVA) was applied and comparison was made through Tukey's test with the least significant

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violet betacyanin (betanin 75–95%) and yellow betaxanthin (vulgaxanthin I ∼ 95%) ( C ai et al., 2005a ). Owing to their hydrophilic nature, betalain compounds can be extracted with pure water, aqueous methanol, or ethanol. However, the extraction method

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solution in ethanol were obtained from Merck (Budapest, Hungary). Methanol, sodium hydroxide, and anhydrous sodium sulphate were obtained from Reanal Laborvegyszer (Budapest, Hungary). 1,2-Bis-palmitoyl-3-chloropropanediol-d 5 internal standard was

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: methanol (99.9%, Sigma-Aldrich, Germany), FeCl 3 (97%, Sigma-Aldrich, Germany), TPTZ 2,4,6-tri(2-pyridyl-1,3,5-triazine) (99%, Acros Organics, China), 35% HCl (p.a., Ing. Petr Švec - PENTA, Czech Republic), CH 3 COONa (p.a., Ing. Petr Švec - PENTA, Czech

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Acta Alimentaria
Authors: Cs. Dobolyi, K. Inotai, I. Bata-Vidács, D. Sárkány, O. Csernus, S. Kocsubé, B. Tóth, A. Szekeres, and J. Kukolya

2 mL dichloromethane. The extracts were stored at −20 °C until analysis. To each culture flasks containing corn grit samples 20 mL methanol was added and the mixtures were transferred into a Stomacher bag. The samples were homogenised for 45 s in a

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dissolved in isooctane, and ester derivative was prepared with 2M KOH in methanol and neutralized with NaHSO 4 . The analysis of fatty acid methyl-esters were performed by GC-MS (Shimadzu, 2010; Supleco-wax column). FAME-mix and Grain-mix standards were used

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