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diagnosis of bTB. Materials and methods Ethics statement Animal and Plant Quarantine Agency (APQA) is a national central research institute in the Republic of Korea that has been researching and developing diagnosis, treatment, and prevention technologies

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2018 ; 56 : 693 – 705 . 10.1007/s12275-018-8238-5 38. Cao Y , Fanning S , Proos S , Jordan K , Srikumar S . A review on the applications of next generation sequencing technologies as applied to food-related microbiome studies . Front

Open access

Indokolt-e a spermaminták bakteriológiai szűrése in vitro fertilizáció előtt?

Is bacteriological screening of semen samples before in vitro fertilization treatment justified?

Orvosi Hetilap
Authors:
Éva Berkes-Bara
,
Annamária Nemes
,
Kata Joó
,
Beáta Dudás
,
Ákos Murber
,
János Urbancsek
, and
Péter Fancsovits

. Microbial contamination in assisted reproductive technology: source, prevalence, and cost. J Assist Reprod Genet. 2020; 37: 53–61. 3 World Health

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Abstract

Endovascular treatment of intracranial aneurysms is a routine medical practice. The most widely used technique is the packing the aneurysm sac with an embolic material. To gain deeper understanding in the effects of specific treatment methods, the intra-aneurysmal haemodynamics are studied with the help of patient-specific computational models. Numerical simulations demonstrated that embolisation with liquid polymer results in an overall decrease of the wall shear stress and pressure in the aneurysm region. Within the range of clinically relevant packing density, simulation of coil embolisation showed homogenisation and decrease of the wall loads on the aneurysm sac. Increasing the packing density above 20% produces little or no further reduction of intra-aneurysmal flow. Sufficient packing of the aneurysm sac results in significant intra-aneurysmal flow decrease associated with reduced wall loads but locally increased pressure or wall shear stress zones may appear depending on the specific vessel geometry.

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A comparison of the results obtained using the classical methods with those of the API 20 Strep system was carried out in identifying 24 enterococci strains isolated from San Simón cow's milk cheese, a traditional Spanish variety. The results of both identification systems coincided exactly in 9 strains (37.5% of the strains studied). In one strain the results obtained using the classical methods did not coincide with those using the API 20 Strep method. 3 strains (12.5%) could not be identified using the API 20 Strep system. However, 11 strains (45%), that remained doubtful between both species E. faecalis and E. faecium on the basis of the classical methods, were identified using the API 20 Strep system. The API 20 Strep system does not include some biochemical tests of importance in identifying of foodborne enterococci and could not identify the atypical strains of Enterococcus. Moreover, this system is adapted to the identification of enterococci of clinical origin and their database does not include some species common in foods. However, it could have an application in combination with the classical methods in order to carry out a reasonably rapid and reliable identification of enterococci related to cheese.

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Abstract

ESBL-producing Escherichia coli strains threaten public health and obligate the use of last-resort antibiotics. This study identified 15 E. coli isolates through 16S rRNA and gyrB genes, specific to E. coli, in 120 egg samples (12.5%). Antibiotic resistance was detected according to the EUCAST and CLSI in E. coli isolates. 2 isolates were susceptible to all antibiotics, one isolate was resistant to one antibiotic, one isolate was resistant to 2 antibiotics, and 11 E. coli isolates (73.3%) had multidrug resistance. Most frequent antibiotic resistances were detected against ampicillin (80%), tetracycline (66.6%), and chloramphenicol (66.6%). A double-disc confirmation test was used to detect ESBL production, and bla TEM, bla SHV, bla CTX-M and bla OXA genes were searched by PCR. The bla TEM (100%) gene was found in all resistant E. coli isolates, and the bla CTX-M gene was detected in only 3 (20%) E. coli isolates. None of the E. coli isolates contained the genes responsible for carbapenem and colistin resistance. Our results show that multi-drug antibiotic resistance and the bla TEM gene are frequent in E. coli from table eggs in Istanbul. This is the first preliminary study on ESBL-producing E. coli isolates in table eggs in Türkiye.

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Considering existing classic and contemporary proposals for preventing online addiction problems: Some old recipes for new problems

Commentary on: Problematic risk-taking involving emerging technologies: A stakeholder framework to minimize harms (Swanton et al., 2019)

Journal of Behavioral Addictions
Author:
Olatz Lopez-Fernandez

technologies. The main contribution of their paper is a preliminary framework of seven groups (i.e. individuals, families, community, treatment and welfare providers, researchers, industry and governments) with a set of roles and responsibilities to minimise

Open access

A termékenységtudatosságon alapuló módszerek lehetőségei a családtervezésben

The possibilities of fertility awareness methods in family planning

Orvosi Hetilap
Authors:
Zoltán Kovács
,
Balázs Bálint
,
Márton Keszthelyi
,
Anna Vizer
,
Csilla Kállay
, and
Henrik Szőke

. Assisted reproductive technology in Europe, 2013: results generated from European registers by ESHRE. Hum Reprod. 2017; 32: 1957–1973. 4 Carson SA

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, trimethoprim-sulfamethoxazole, and colistin. Whole-genome sequencing The whole-genome sequencing was performed using DNA nanoball sequencing technology as previously described [ 8 ]. In brief, genomic DNA isolated from Kae1177-1bg was randomly size fragmented

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