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2018 ; 56 : 693 – 705 . 10.1007/s12275-018-8238-5 38. Cao Y , Fanning S , Proos S , Jordan K , Srikumar S . A review on the applications of next generation sequencing technologies as applied to food-related microbiome studies . Front

Open access

Considering existing classic and contemporary proposals for preventing online addiction problems: Some old recipes for new problems

Commentary on: Problematic risk-taking involving emerging technologies: A stakeholder framework to minimize harms (Swanton et al., 2019)

Journal of Behavioral Addictions
Author:
Olatz Lopez-Fernandez

technologies. The main contribution of their paper is a preliminary framework of seven groups (i.e. individuals, families, community, treatment and welfare providers, researchers, industry and governments) with a set of roles and responsibilities to minimise

Open access

Abstract

Endovascular treatment of intracranial aneurysms is a routine medical practice. The most widely used technique is the packing the aneurysm sac with an embolic material. To gain deeper understanding in the effects of specific treatment methods, the intra-aneurysmal haemodynamics are studied with the help of patient-specific computational models. Numerical simulations demonstrated that embolisation with liquid polymer results in an overall decrease of the wall shear stress and pressure in the aneurysm region. Within the range of clinically relevant packing density, simulation of coil embolisation showed homogenisation and decrease of the wall loads on the aneurysm sac. Increasing the packing density above 20% produces little or no further reduction of intra-aneurysmal flow. Sufficient packing of the aneurysm sac results in significant intra-aneurysmal flow decrease associated with reduced wall loads but locally increased pressure or wall shear stress zones may appear depending on the specific vessel geometry.

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A comparison of the results obtained using the classical methods with those of the API 20 Strep system was carried out in identifying 24 enterococci strains isolated from San Simón cow's milk cheese, a traditional Spanish variety. The results of both identification systems coincided exactly in 9 strains (37.5% of the strains studied). In one strain the results obtained using the classical methods did not coincide with those using the API 20 Strep method. 3 strains (12.5%) could not be identified using the API 20 Strep system. However, 11 strains (45%), that remained doubtful between both species E. faecalis and E. faecium on the basis of the classical methods, were identified using the API 20 Strep system. The API 20 Strep system does not include some biochemical tests of importance in identifying of foodborne enterococci and could not identify the atypical strains of Enterococcus. Moreover, this system is adapted to the identification of enterococci of clinical origin and their database does not include some species common in foods. However, it could have an application in combination with the classical methods in order to carry out a reasonably rapid and reliable identification of enterococci related to cheese.

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A termékenységtudatosságon alapuló módszerek lehetőségei a családtervezésben

The possibilities of fertility awareness methods in family planning

Orvosi Hetilap
Authors:
Zoltán Kovács
,
Balázs Bálint
,
Márton Keszthelyi
,
Anna Vizer
,
Csilla Kállay
, and
Henrik Szőke

. Assisted reproductive technology in Europe, 2013: results generated from European registers by ESHRE. Hum Reprod. 2017; 32: 1957–1973. 4 Carson SA

Open access

, trimethoprim-sulfamethoxazole, and colistin. Whole-genome sequencing The whole-genome sequencing was performed using DNA nanoball sequencing technology as previously described [ 8 ]. In brief, genomic DNA isolated from Kae1177-1bg was randomly size fragmented

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Acta Microbiologica et Immunologica Hungarica
Authors:
Yussef Moghaddam
,
Hajar Ziaei Hezarjaribi
,
Abdol Sattar Pagheh
,
Mahdi Fakhar
,
Reza Saberi
,
Mitra Sharbatkhori
,
Mahbobeh Montazeri
,
Hossein Ghalehnoei
, and
Eisa Nazar

of CL was confirmed by the presence of amastigotes in Giemsa-stained smears. In order to determine drug sensitivity, amastigotes were cultured in Novy-MacNeal-Nicolle (NNN) medium and RPMI 1640 media (Gibco, Life Technologies GmbH, Germany

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A bioluminescent derivative of Bacillus subtilis containing a plasmid encoding a luxAB fusion under control of a vegetative promoter and gives bioluminescence upon addition of an exogenous long-chain aldehyde has been used as test organism. Its spore populations have been produced and their heat- and radiation survival curves established. Heat-sensitization effect of pre-irradiation of spores was proven not only by colony counting but also with differential scanning calorimetry. Under a linearly programmed temperature increase, the heat destruction of spores surviving 2.5 kGy gamma irradiation resulted in at a few centigrade lower temperature than that of untreated spores. Heat denaturation endotherms in the DSC-thermogram of irradiated spores were shifted to lower temperatures as well. Comparative turbidimetric, luminometric and phase-contrast microscopic studies of untreated, heat-treated and irradiated spore populations showed that the kinetics of germination and the light emission during germination of radiation-inactivated spores were the same as those of untreated spores, revealing that the pre-formed luciferase enzyme packaged into the spores during sporulation remained intact after an irradiation dose causing 90% decrease in number of colony forming spores. Therefore, in contrast to heat-treated spores, the initial bioluminescence reading upon germination of irradiated spores does not reflect the viable count of their population.

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Abstract

Background and aims

This scoping review employed a multifaceted conceptualization of well-being to examine how psilocybin use affects well-being and related sub-concepts in healthy individuals. It investigated which factors influence the relationship between psilocybin use and well-being, what research protocols have been employed, and what underlying mechanisms have been proposed in existing studies.

Methods

A comprehensive literature search in line with the PRISMA guidelines was conducted. Scopus, PubMed, PsycINFO, Web of Science, and Google Scholar were searched for peer-reviewed articles about psilocybin and well-being in healthy populations.

Results

Studies were heterogeneous in regard to study objectives, study design, study procedure, sample size and psilocybin dosage. In all studies, psilocybin use led to positive well-being-related outcomes for the majority of participants. Facets of well-being positively affected by psilocybin use in this review were self-acceptance, positive relationships, and meaning/purpose in life.

Conclusions

This scoping review provided preliminary evidence for the beneficial effects of psilocybin on well-being and related sub-concepts such as self-acceptance, positive relationships, and meaning/purpose in life in healthy individuals. Ego-dissolution, unity, connectedness, and mystical-type experiences are interrelated concepts that seem to be crucial for explaining such positive well-being-related effects of psilocybin. Under conducive conditions, the use of psilocybin may contribute to healthy functioning, through broad and sustained improvements in a variety of well-being concepts. Due to the heterogeneous nature of the studies, more definite conclusions require further research with a rigorous and homogeneous design.

Open access