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Table 1 . IFN- γ detection in the supernatant of cultured PBMCs using ELISA IFN-γ in the culture supernatant was assessed by using a commercial ELISA kit (Human IFN gamma ELISA Ready-SET-Go, ebioscience, USA) (sensitivity: 4 pg/mL) based on the
other abnormalities and diseases. None of them was pregnant neither was any on contraceptives. Laboratory tests ITIH4 and haptoglobin were measured using two ELISA Sandwich tests (Acuvet Biotech, Zaragoza, Spain) that were previously developed and
) was purchased from Beijing Solarbio Science & Technology Co., Ltd (Beijing, China). The activity of myeloperoxidase (MPO) was analyzed by the MPO Activity assay kit (Cat no:ab105136, Abcam). The Mouse TNF-α ELISA kit (Cat No: SEKM-0034) and the
analysis These samples were tested for the presence of group-specific antibodies against BTV using a commercially available competitive enzyme linked immunosorbent assay (c-ELISA) (ID Screen Bluetongue Competition ELISA, IDvet, France) as per manufacturer
collected and stored at −20 °C until performing enzyme-linked immunosorbent assay (ELISA). Cells were washed with HT-29 cell culture medium and lysed with TRI Reagent (Sigma-Aldrich). Lysed cells were collected and stored at −80 °C until real-time time
Antibodies on the SARS-CoV-2 virus were detected using the automated “Sandwich” enzyme-linked immunosorbent assay (ELISA) method with Euroimmun ELISA Analyser I-2P (EUROIMMUN Medizinische Labordiagnostika AG, Lübeck, Germany). The samples were tested using
–3 2,600 54 7 825 266 3.1 9 (3.4%) 14 (1.7%) 1–2 1,700 49 8 665 253 2.6 8 (3.2%) 13 (2.0%) 1–3 1,200 55 Total 16,589 3,393 4.9 127 (3.7%) 291 (1.8%) The sera were tested by indirect ELISA (iELISA) for the presence of serum antibodies against S. equi
). ELISA For detecting the TNF-α, IL-6, IL-1β protein levels, ELISA kits (TNFα kit, ab100785; Abcam; IL-6 kit, ab100772; Abcam; IL-1β kit, ab100768; Abcam) were used. The production of TNF-α, IL-6, and IL-1β in the hippocampus tissue of rats
reader (Epoch Microplate Spectrophotometer, Bio Tek, USA). NO production was then calculated by comparing the OD values of the samples with the standard curve. ELISA assay Soluble intercellular adhesion molecule-1 (sICAM-1) and soluble vascular cell
time of measurements. After collection of all samples, serum copeptin levels were measured using “Human Copeptin ELISA Kits” (Phoneix Pharmaceuticals, USA; catalog no.: EK-065-32; lot no.: 603.858)” in microplate readers (Bio-Tek Instruments ELx800, USA
