Search Results
-Aldrich) to purple formazan in viable cells. The optical density was determined at a wavelength of 570 nm against 620 nm as reference using a microplate spectrophotometer ELISA reader (Multiscan FC, ThermoFisher Scientific, Vantaa, Finland). The data were
. Baydın , M. and Bilge-Dagalp , S. ( 2017 ): Investigation of the seroprevalence of BoHV-1 and CpHV-1 infections using gB/gE ELISA combination and VNT in selected goat
, Verbakel H , Bergmans AM , Roord JJ , van Dijken PJ , . Serological testing for Bartonella henselae infections in the Netherlands: clinical evaluation of immunofluorescence assay and ELISA . Clin Microbiol Infect 2007 ; 13 : 627 – 34
laboratory methods (ELISA, PCR) in any age group. Vaccination during the fattening process (including lactation, nursery, fattening) is not allowed in these farms; therefore, the tests must not show seropositivity in the progeny, and all animals must be free
and musculoskeletal malformations, especially arthrogryposis and hydranencephaly. In addition, inflammatory responses may also develop. Real-time PCR, virus isolation and antibody ELISA are reliable diagnostic techniques ( Beer et al., 2013 ; Conraths
rotavirus strain surveillance for the common serotypes in circulation, both serological and molecular methods have been used. The tests included virus cultivation, Electron Microscopy (EM), Enzyme-Linked Immunosorbent Assay (ELISA), Latex Agglutination Test
concentrations of the blood serum were analysed by a ROCHE Cobas e 411 fully automated immunoassay analyzer, using Rat Leptin ELISA Kit (Sigma, RAB0335), Rat Ins1/Insulin ELISA Kit (Sigma, RAB0904) and Rat Glucagon EIA Kit (RayBioTech, P06883) for serum, plasma
laboratory. Then, they were thoroughly mixed and pooled just before RNA and DNA-RNA isolation. First, we examined clinical samples for the FCV virus by ELISA and reverse transcription polymerase chain reaction (RT-PCR) assay; these results were reported in
30 min. The 150 µL of 33% glacial acetic acid per well was used to dissolve the stain-attaching biofilm for 30 min. The biofilm development was assessed using a 96-well ELISA reader to measure the absorbance at 595 nm (Multiscan FC, Thermo Fisher
ELISA reader was used to spectrophotometrically measure the optical density (OD) of the previously stained attached biofilm at 490 nm wavelength. The results were then interpreted as previously explained elsewhere [ 9 ]. Staphylococcus epidermidis ATCC
