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desorption ionization-time of flight mass spectrometer (MALDI-TOF MS) (Bruker Daltonics, Bremen, Germany). After the isolation and identification, the bacteria were stored at −80 °C in Brain Heart Infusion (BHI) broth (Oxoid, UK) supplemented with 12% (v

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was accepted. Bacterial identification was confirmed with Vitek 2 ID/AST (bio-Mérieux, France) automated bacterial identification system and MALDI-TOF MS (bio-Mérieux). Isolates were stored in brain–heart infusion broth containing 10% glycerol at −20

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. Identification at species level was performed by biochemical tests such as indole test, methyl-red and Voges-Proskauer test and citrate test, Vitek-2 (Biomérieux, France) and matrix-assisted laser desorption/ionisation (MALDI-TOF) mass spectrometer. Bacterial

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. 18. Puglisi , C , Samperi , F , Cicala , G , Recca , A , Restuccia , CL . Combined MALDI-TOF MS and NMR characterization of copoly(arylen ether sulphone)s . Polymer . 2006 ; 47 : 6 1861 – 1874 . 10.1016/j

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. 21. Osman Z , Pizzi A . Structural elucidation of polymeric condensed tannins of Acacia nilotica spp . by 13CNMR and MALDI–TOF analyses. Presented in 8th annual meeting of bioenvironmental

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, Brno, Czechia), PCR method, and MALDI-TOF spectroscopy with score 2.397. 2.2 Inoculation and cultivation conditions The isolate was kept in BHI broth (Sigma-Aldrich, St. Louis, USA) at 5 ± 1 °C prior to analysis. Preparation of a standard suspension and

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, G. , Karagöz , A. and Parlak , M. ( 2019 ): From days to hours: can MALDI-TOF MS system replace both conventional and molecular typing methods with new cut off level for vancomycin resistant Enterococcus faecium . J. Microbiol. Methods 162

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and 2019 were included in our study. The identification and antibiotic susceptibility tests of the strains were performed using MALDI-TOF MS (bioMérieux, France) and Vitek-2 (bioMérieux, France) system. BMD reference method Reference BMD test was

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Acta Microbiologica et Immunologica Hungarica
Authors: Serra Örsten, Selay Demirci-Duarte, Tuğçe Ünalan-Altıntop, Aslı Çakar, Banu Sancak, Koray Ergünay, and Cumhur Özkuyumcu

MALDI-TOF MS (Biomerieux, France). Baseline antibiotic susceptibility patterns were evaluated by VITEK2 ID/AST and/or BD Phoenix NMIC-400/ID panel (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md, USA) when required. The tested antibiotics

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, USA). The mass spectrometric analysis was performed on a 4800 MALDI TOF/TOF Analyzer (AB Sciex, Framingham, USA). The mass spectra of peptides were acquired from 800 to 4,000 m/z window in the reflector positive ion mode first, followed by tandem mass

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