Search Results
]. There is no effective bioanalytical method available to quantify panasenoside in biological samples to date. Compared to liquid chromatography tandem mass spectrometry (LC–MS/MS), ultra-performance liquid chromatography (UPLC)–MS/MS is more sensitive and
models, determined using LC–ion trap MS, has not yet been reported. Therefore, in the present study, a simple, rapid, and selective UPLC–ion trap MS method for the quantification of atractylenolide III in the rat plasma samples after oral
), orthophosphoric acid (88% w/w ACS grade), Acetonitrile (Merck-HPLC grade), Hydrochloric acid 36% (AR grade), and Water (Milli-Q grade). Instrumentation and methodologies Analytical UPLC chromatographic conditions Chromatographic separation was performed on Water
analysis. Ultra performance liquid chromatography–electrospray ionization–tandem mass spectrometry (UPLC-ESI-MS/MS) analysis Analyses were performed using a Waters UPLC Acquity System coupled to a Quattro Premier tandem MS
modern forms to ensure the consistency of clinical efficacy with the traditional formula. An integrated strategy was developed by coupling ultra-performance liquid chromatography (UPLC), quadrupole time-of-flight (Q-TOF) with hybrid triple
goats ( El-Hewaity et al . , 2014 ). Acetonitrile, methanol and ammonium acetate for UPLC were purchased from Sigma-Aldrich (St. Louis, MO, USA). The product was approved for the treatment of pneumonia in cattle and pigs as well as mastitis
biologically tested sample we want for UPLC-MS analysis. Hemostatic activity test According to the previously-scheduled plasma collection point, SD rats from each group were given orally to corresponding drugs, and then anticoagulated blood was collected
Summary
A simple and rapid method, using online ultraperformance liquid chromatography with photodiode array detection and electrospray ionization mass spectrometry (UPLC-PDA-eλ-ESI-MS/MS), was developed for the in-depth analysis of 50 batches Radix et Rhizoma Rhei. The analysis was performed on a UPLC BEH C18 column using a gradient elution system. Baseline separation could be achieved in less than 7.5 min. At the same time, on the basis of the 50 batches of samples collected from representative cultivated regions, a novel chromatographic fingerprint was devised by UPLC-PDA, in which 27 common peaks were detected and identified by the developed UPLC-MS/MS method step by step according to fragmentation mechanisms, MS/MS data, standards, and relevant literature. Many active components gave prominent [M - H]− ions in the ESI mass spectra. These components include anthraquinones, sennosides, stilbenes, glucose gallates, naphthalenes, and catechins. Furthermore, based on the information of these Radix et Rhizoma Rhei components, and further combined with discriminant analysis, a novel discriminant analysis equation (DAE) was established for the quality control of Radix et Rhizoma Rhei for the first time.
Summary
A new method using ultra-performance liquid chromatography (UPLC) in combination with tandem mass spectrometry and a multiple reaction monitoring mode (UPLC-MS/MS-MRM) was developed for simultaneous quantitative determination of anthraquinone derivatives in Radix et Rhizoma Rhei-based medicines. A multi-mode electrospray/chemical ionization (ESCI) and negative ion mode with [M-H]− and its fragments under collision-activated conditions were employed in MS/MS-MRM. The quantitative method was validated and applied to simultaneous determination of anthraquinone derivatives in 21 Radix et Rhizoma Rhei-based medicines. The limits of quantification were in the range of 3.90–9.09 ng mL−1. Average recoveries were between 95.5% and 99.8% with relative standard deviations from 1.8% to 5.3%.
Compound danshen preparations (CDPs) are used clinically for the treatment of cardiovascular and cerebrovascular diseases. By using the quantitative analysis of multi-components by single-marker (QAMS) method, sixteen compounds (danshensu, protocatechuic acid, protocatechuicaldehyde, caffeic acid, rosmarinic acid, lithospermic acid, notoginsenoside R1, salvianolic acid B, ginsenoside Rg1, ginsenoside Re, salvianolic acid A, salvianolic acid C, ginsenoside Rb1, ginsenoside Rd, cryptotanshinone, and tanshinone IIA were quantified on an ACQUITY ultraperformance liquid chromatography (UPLC) HSS T3 column (2.1 × 100 mm, 1.8 μm) with the mobile phase consisting of 0.1% formic acid aqueous solution (A) and acetonitrile (B) using a gradient elution at the flow rate of 0.30 mL/min in 30 min at 30°C, which was also validated by UPLC-diode array detection (DAD) and UPLC-electrospray ionization multistage/mass spectrometry (ESI-MS/MS) for assuring the feasibility and accuracy. Tested by robustness experiment under slightly changeable conditions, the stability of relative correction factor (RCF) proved to be stable, with RSDs below 5.69%, except for notoginsenoside R1 with relative standard deviation (RSD) 7.83%. This reliable and convenient QAMS method resolved the problem of standard substance insufficiency and improved the quality assessment of preparations consisting of complex compounds with different chemical structures, such as CDPs.
