It is well established that the ingestion of cereal prolamins, such as gluten, causes the characteristic symptoms of celiac disease (CD) in people predisposed to it. DNA-based PCR method provides new ways to detect gluten in processed foodstuffs, such as bread. The aim of this work was to adapt a new primer pair combination and to initiate a carefully elaborated PCR methodology to experiment with DNA-based analysis. At first, the purity of cleaned DNA was verified using B49317 and A49855 chloroplast DNA primer pair. Then TR01/2 wheat specific PCR primer pair was used for checking the origin of the DNA, and P1/2 microsatellite (SSR) adapted primer pair for detecting allergen (gluten) specific residues. Method optimisation was achieved with cereal flour samples, then bread and dry pasta products from wheat were used, which were analysed as heat-treated samples with three primer pairs. The gluten specific primer pair was tested on cross-reactive cereals such as rye, barley, triticale and on some questionable cereals, such as oat, and pseudo-cereals, e.g. buck wheat and amaranth.
Krol, M., Griffith, M., and Huner, N. P. A. (1984): An appropriate physiological control for environmental temperature studies: comparative growth kinetics of winter rye. Can. J. Bot. , 62, 1062-1068.
Kishore, N., Chaudhary, H.K., Chahota, R.K., Kumar, V., Sood, S.P., Jeberson, S., Tayeng, T. 2011. Relative efficiency of the maize and Imperata cylindrica-mediated chromosome elimination approaches for induction of haploids of wheat-rye derivatives. Plant
Forsström, P.O., Merker, A., Schwarzacher, T. 2002. Characterization of mildew resistant wheat-rye substitution lines and identification of an inverted chromosome by fluorescent in situ hybridization. Heredity
The aim of the study was to determine the characteristics of Szabolcs-Szatmár-Bereg county in North-Eastern Hungary and to elaborate an environmentally sound agricultural strategy. 50% of Szabolcs-Szatmár-Bereg county is arable land (303,950 ha). After the change of regime there was no significant decrease in the size of the arable land. Cultivation is still practised on soils of poorer quality. Under the changed production conditions, crop cultivation in its present form does not come up to the economic expectations. The sowing structure is not suited to the regional climate and soil conditions; 75% of the arable land is occupied by only 5 species of plant. A change in the cultivation profile (afforestation) can be expected on connected marginal areas where economic calculations indicate that economical cultivation is not possible. A typical feature of the region's cereal production is that it takes up significantly more arable land than is justified: in recent years the average ratio of cereals was 60–65%. In certain microregions the production of protein fodders is recommended for the economical production of better quality meat. There has been an increase in the area sown to triticale, peas, beans and cucumber for seed production. The production of flowers, vegetables, spices and herbs should be promoted; these plants require manual work, so they could play a significant role in solving employment problems. On more unfavourable areas, suitable for the cultivation of agricultural products, the production of low-input plants (e.g. rye, triticale, sorghum) suitable for extensive cultivation should be considered. In small regions with favourable ecological and infrastructural situations the area sown to crops with greater production value and greater labour requirements can be expected to increase.
Fluorescence in situ hybridization (FISH) can reveal minor structural differences of chromosomes. The karyotype of common wheat (Triticum aestivum L.) based on FISH pattern is seldom reported. In this study, non-denaturing FISH (ND-FISH) using Oligo-pSc119.2-1, Oligo-pTa535-1 and (AAG)6 as probes was used to investigate the chromosomal structure of 85 common wheat including 83 wheat-rye 1RS.1BL translocation cultivars/lines, a wheatrye 1RS.1AL translocation cultivar Amigo and Chinese Spring (CS). Two, three, two, three, six, three and four structural types respectively for 1A, 2A, 3A, 4A, 5A, 6A and 7A chromosomes were observed. Two, eight, two, two, four and six types of chromosome for 2B, 3B, 4B, 5B, 6B and 7B were respectively detected. The structure of 1B chromosomes in Amigo and CS is different. Five, two, two and two types of chromosomal structure respectively for 1D, 2D, 3D and 5D were distinguished. Polymorphisms of 1RS.1BL, 4D, 6D and 7D chromosomes were not detected. Chromosomes 1AI, 2AI, 3AI, 4AI, 5AIII, 6AI, 7AIII, 2BI, 3BV, 4BI, 5BII, 6BIII, 7BI, 1DIV, 2DI, 3DI and 5DII appeared in these 85 wheat cultivars/lines at high frequency. Each of the 85 wheat cultivars/lines has a unique karyotype. Amigo is a complex translocation cultivar. The FISH karyotype of wheat chromosomes built in this study provide a reference for the future analyzing wheat genetic stocks and help to learn structural variations of wheat chromosomes. In addition, the results in this study indicate that oligonucleotide probes and ND-FISH technology can be used to identify individual wheat cultivar.