Ten different strains of
, isolated from composting soils were found to produce phytase when grown on PSM medium. The wild type strain CM was found to produce maximum amount of phytase (4.33 units/g DW substrate). Culturing
strain CM on medium containing wheat bran and optimizing other culture conditions (carbon source, media type, nitrogen source, level of nitrogen, temperature, pH, inoculum age, inoculum level and moisture), increased the phytase yield to 13.26 units/g substrate. This culture was further subjected to UV mutagenesis for developing phytase hyperproducing mutants. The mutant (TL-7) showed 2.29-fold increase in phytase activity as compared to the parental strain. Employing Box-Behnken factor factorial design of response surface methodology resulted in optimized phytase production (32.19 units/g of substrate) by mutant TL-7. A simple two-step purification (40.75-folds) of phytase from mutant TL-7 was achieved by anion exchange and gel filtration chromatography. The purified phytase (∼54 kDa) was characterized to be optimally active at pH 5.0 and temperature 70 °C, though the enzyme showed ∼70% activity over a wide pH and temperature range (2.0–10.0 and 30–90 °C, respectively). The phytase showed broad substrate specificity with activity against sodium phytate, ADP and riboflavin phosphate. The phytase from
was thermoacidstable as it showed up to 70% residual activity after exposure to 70 °C at pH 3.0 for 120 min. The enzyme showed K
4.55 μM and V
0.833 μM/min/mg against sodium phytate as substrate.
Authors:V. Jurkovich, J. Kutasi, Hedvig Fébel, J. Reiczigel, E. Brydl, L. Könyves, and P. Rafai
. and Vrasanska, M. (1998): Biochemical and catalytic properties of an endoxylanase purified from the culture filtrate of Thermomyceslanuginosus ATCC 46882. Carbohydr. Res. 306 , 445-455.
Biochemical and catalytic properties
Arnesen, S., Eriksen, S. H., Olsen, J., Jensen, B. (2002) De novo synthesis is involved in production of extracellular α-amylase activity from
in stationary phase.
Mycol. Res. 106
Authors:J. M. Rezessy-Szabó, E. Bujna, and Á. Hoschke
Rezessy-Szabó, J. M., Nguyen, D. Q. & Hoschke, Á. (2000): Formation of α-galactosidase enzyme by Thermomyceslanuginosus. Fourteenth Forum for Applied Biotechnology. 27-28 September 2000. Gent, Belgium Proceedings part I, pp. 319
Authors:Abeer H. Ali, Usama Radwan, Soad El-Zayat, and Magdi A. El-Sayed
). Thermomyceslanuginosus was first isolated from soil and involved in compost degradation until its first isolation as plant endophyte ( Novas & Carmarán, 2008 ). The plants under extreme temperatures exhibit various symptoms like chlorosis and necrosis
Authors:V. Jurkovich, E. Brydl, P. Rafai, and et al.
Purkarthofer, H. and Steiner, W. 1995: Induction of endo-beta-xylanase in the fungus Thermomyces lanuginosuspp. Enzyme Microb. Technol. 17 , 114-118.
Induction of endo-beta-xylanase in the fungus Thermomyceslanuginosus
Authors:Mária Óbert, Antalné Csepregi, Katalin Posta, Erika Tóth Király, and László Hornok
lignocellulóz-tartalmú növényi hulladékból álló, termofil fázisban lévő
komposzthalmokból 21 gombát izoláltunk. A morfológiai és molekuláris
módszerekkel azonosított gombák tíz fajba tartoztak, hat
termofil fajba (
Aspergillus fumigatus, Aspergillus versicolor,
Rhizomucor pusillus, Thermoascus aurantiacus, Talaromyces thermophilus,
) és négy mezofilbe (
Aspergillus terreus, Neosartorya fischerii és Trichoderma hamatum
). A két
leggyakoribb faj a
előbbit nyolc, az ut_