Authors:G. Szabó, T. Müller, M. Bercsényi, B. Urbányi, B. Kucska, and Á. Horváth
Experiments were carried out on the sperm cryopreservation of artificially induced eels. The effects of several extenders and two cryoprotectants on the motility of spermatozoa were investigated. The highest post-thaw motility was observed with the combination of Tanaka's extender and DMSO as cryoprotectant. Further dilution after thawing resulted in complete loss of motility in samples frozen in presence of DMSO while sperm frozen with methanol as cryoprotectant retained its motility after further dilution.
Chapman, J. D., Doren, S. D., Reuvers, A. P., Gillespie, C. J., Chatterjee, A., Blakely, E. A., Smith, K. C., Tobias, C. A. (1979) Radioprotection by DMSO of mammalian cells exposed to X-rays and to heavy charged
Authors:Xiang-Rong Xu, Fu-Qing Tan, Jun-Quan Zhu, Ting Ye, Chun-Lin Wang, Yi-Feng Zhu, Hans-Uwe Dahms, Fan Jin, and Wan-Xi Yang
We used single-cell gel electrophoresis (SCGE) to detect the integrity of sperm DNA of the teleost large yellow croaker, Pseudosciaena crocea, cryopreserved with Cortland solution and a range of 5% to 30% DMSO concentrations in order to test how sperm cryopreservation affected the DNA stability of nuclei. Electrophoresis was conducted for 60 min at 130 mA and 15 V. The comet images were analyzed with software CometScore 1.5, and parameters such as comet length, tail length and percentage DNA in the tail were obtained. Then the comet rate and damage coefficient were calculated. Results demonstrated that there were no significant differences in motility, comet rate and damage coefficient between fresh sperm and cryopreserved sperm stored in 5%, 10%, 15% and 20% DMSO, while the sperm cryopreserved with 25% and 30% DMSO had a lower motility, higher comet length and damage coefficients than those of fresh sperm. There was a positive correlation between comet rate of cryopreserved sperm and the concentration of DMSO. Our results demonstrate that toxicity of the cryoprotectant is the main cause of DNA damage in cryopreserved sperm nuclei.
Authors:Z. Bokor, T. Müller, M. Bercsényi, L. Horváth, B. Urbányi, and Á. Horváth
Experiments were carried out on sperm cryopreservation of two European percid fish species, the pikeperch
and the Volga pikeperch
. Two experiments were conducted on pikeperch sperm. In the first, the effects of three extenders (Glucose, KCl, Sucrose) and two cryoprotectants (dimethyl-sulfoxide: DMSO, methanol: MeOH) were tested on motility and fertilization. In the second, the effects of two dilution ratios (1: 1, 1: 9) and two cryoprotectants (DMSO, MeOH) on hatching were investigated. In the experiment on Volga pikeperch the suitability of using cryopreservation for fertilization was investigated. In the first experiment on pikeperch the highest post-thaw motility (28 ± 21%) and fertilization rate (43 ± 12%) was found with DMSO as cryoprotectant in combination with Glucose extender. In the second, the highest hatch rate (41 ± 22%) was observed with MeOH as cryoprotectant and 1: 1 sperm dilution ratio, however no significant difference was found among the results. In the experiment on Volga pikeperch hatch rates with cryopreserved sperm (60 ± 2%) did not significantly differ from the control (60 ± 6%). Contamination of sperm with urine seems to be a key problem in the success of sperm cryopreservation of these species.
Authors:Silke Dubbert, Birgit Klinkert, Michael Schimiczek, Trudy M. Wassenaar, and Rudolf von Bünau
dimethyl sulfoxide (DMSO) was used at 10 μg/plate for reporter strain TA98 and at 40 μg/plate for reporter strain TA1537. The positive control for reporter strain TA102 without metabolic activation was 1 μL/plate methyl methanesulfonate. The positive
Authors:Jakob Knorr, Steffen Backert, and Nicole Tegtmeyer
-Millipore, #2613) was diluted in dimethyl sulfoxide (DMSO) to 10 mM. AGS cells were grown to 70% confluency and washed twice with PBS and fresh medium as described above. Thirty minutes before the cells were infected with H. pylori , they were treated with NSC
Authors:L. Brindzová, M. Zalibera, T. Jakubík, M. Mikulášová, M. Takácsová, S. Mošovská, and P. Rapta
This study examined the mutagenic, antimutagenic and antioxidant activities of the DMSO extracts from the wheat bran. Wheat bran extracts showed no genotoxicity toward
TA98, TA100 and TA102 with or without S9 mix (an external metabolic system). In addition, wheat bran extracts expressed a dose-depend inhibitory effect on the mutagenicity of promutagen aflatoxin B1 (AFB1), an indirect mutagen which requires metabolic activation, and 3-(5-nitro-2-furyl)acrylic acid (5-NFAA), 2-nitrofluorene (2NF) and hydrogen peroxide (H
), direct mutagens, in
TA98, TA100 and TA102 strains. Significant total antioxidant capacity of wheat bran extract was found by two standard spectroscopic assays based on ABTS and DPPH reagents. A special attention was focused to the reactive radical scavenging capacity of bran extract as one of its antioxitant activities. Wheat bran extract possessed higher ability to scavenge oxygen- and carbon-centered reactive radicals generated by the thermal decomposition of K
than BHT (70 and 65% scavenged radicals, respectively) during the electron paramagnetic resonance (EPR)/spintrapping test. The total phenolic content of wheat bran samples expressed in gallic acid equivalent was 2.7 mg/g, total flavonoid content expressed in rutin equivalent was 70.8 μg/g and the most abundant phenolic acids established by GC-MS method were isoferulic (3-hydroxy-4-metoxycinnamic) and ferulic (4-hydroxy-3-metoxycinnamic) acid, sinapic, caffeic,
-coumaric and vanillic acids.
Authors:Cheimâa Bouchekouk, Fatima Zohra Kara, Ghania Tail, Fairouz Saidi, and Tarek Benabdelkader
, 2014 ). Solutions were prepared by serial dilution of P. aquilinum EO in 10% of dimethyl sulfate (DMSO) to obtain concentrations decreasing from 40 to 0.315 μl/ml. Disks impregnated with 12.5 μl of the solutions under test were placed on the agar
Authors:Gergely Sámuel Bartha, Gergő Tóth, Péter Horváth, Eszter Kiss, Nóra Papp, and Monika Kerényi
measured and then chloroform, ethyl acetate, and butanol were used for further extraction. Dried residues were dissolved with dimethyl sulfoxide (DMSO; Sigma-Aldrich). Further dilutions were performed in Mueller–Hinton broth to reach the appropriate
Authors:Mughal Qayum, Muhammad Nisar, Abdur Rauf, Imran Khan, Waqar Ahmad Kaleem, Muslim Raza, Nasiara Karim, Munawar Ahmad Saleem, Saud Bawazeer, Sengul Uysal, Gokhan Zengin, Saqib Jahan, and Mohamed Fawzy Ramadan
lines (2 × 10 4 and 9 × 10 3 ) mice hepatocytes stayed planted in 96-well plates. The cells were preserved with the compounds (1.5–100 μM) or vehicle (0.2% DMSO) and hatched for 48 hr. It was tracked by MTT (3-[4,5-dimethylthiazol-2-yl]-2, 5