A simple and sensitive method of high-performance liquid chromatography with fluorescence detection (HPLC-FLD) was developed for the determination of icariin in capsules by precolumn chelation with aluminum. In order to obtain a stable fluorescence signal, the reaction conditions of the fluorescent chelation complex between icariin and aluminum were investigated in detail. Chromatography was carried out on an Agilent Zorbax Extend C18 column (150 mm × 4.6 mm, 5.0 μm) using methanol as mobile phase at a flow rate of 1.0 mL min−1. The excitation and emission wavelengths were set at 430 and 480 nm, respectively. At optimum conditions, the calibration curve was linear in the concentration range from 0.010 to 100.0 μg mL−1 with the limit of detection of 3.5 ng mL−1 (S/N = 3). A comprehensive method was validated for precision and accuracy. The method described here has been successfully applied for the determination of the icariin content in a capsule with satisfactory results.
A simple, rapid, and sensitive reversed-phase HPLC method was developed and validated for determination of metronidazole and tinidazole in human plasma samples under identical chromatographic conditions. This method involves liquid-liquid extraction using chloroform: isopropylalcohol (95:5). Chromatographic separation was performed using a μ-bondapack C18 (250 mm × 4.6 mm) column. The mobile phase consisted of potassium dihydrogen phosphate solution (0.005 M)/acetonitrile (80/20 v/v). The final pH of the mobile phase was adjusted to 4 ± 0.1 with orthophosphoric acid. The calibration curves were linear over the concentration range 0.1–15 μg/mL for metronidazole and tinidazole with the detection limit of 30 ng/mL. Within- and between-day precision and accuracy did not exceed 9.83% and 10.48%, respectively. Metronidazole and tinidazole were found to be stable in plasma samples with no evidence of degradation during 3 freeze-thaw cycles and 3 months storage in −70 °C. The current validated bio-analytical method was finally applied in bioequivalence studies of two different metronidazole and tinidazole products according to a standard two-way cross-over design with a two-week washout period. No statistically significant difference was observed between the logarithmically transformed AUC0-∞ and Cmax values. Therefore, generic products were considered bioequivalent with those of standards which could be used interchangeably.
In October 2012 the workshop entitled “Reading Past and Present Landscapes in Central Europe” was held in Hungary. During the workshop, which was focused on exchanging ideas and experience concerning remote sensing methods of detection and registering archaeological sites, a large Roman site near the village of Sárbogárd in Nagyhörcsökpuszta was detected. The area of the site, its location, and the finds suggest that it could have played an important role in the region. This paper presents the results obtained from geophysical prospection and field walking, as well as the results of pottery analysis.
Research of the manuscript hitherto known and labeled as the "Liber sequentiarum and sacramentarium" (“LS”), stored today in Šibenik, has shown that this chant book is the earliest missal copied in the monastery of Tegernsee for the St Thomas Basilica in medieval Pula, Istria.Indications for its provenance are drawn from its codicological, palaeographical and repertorial features. Beside the detailed comparison of its script, notation, sequence and trope repertory, this article shows up to date not analyzed repertory of the saints venerated in its sacramentary and in the list of relicts to be mentioned at the end of missal (Haec suntreliquiae). Particularly, this portions of the “LS” repertory were a clue toward detection that the “Leitheiliger” of this chant book is St Thomas Apostle, a patron of the bishopric of Parenzo/Pula, to whom also the “unicum” sequence Armonia concinnans was dedicated. St Thomas Basilica in medieval Pula is place of usage where this “commissioned book” has been meant for liturgical usage. In the broader Aquileian context this manuscript is important as a “new source” from Aquileia and its eastern province of Istria.
Imre Steindl (1839–1902) is thought to be one of the most prominent architects of the Hungarian Historicism, whose active contribution to the Hungarian Neo-gothic architecture and restoration practices can hardly be overestimated. Albeit, his activity as an architect of the renowned late chief work of the international Gothic Revival, the Hungarian Parliament, as a leader of a prosperous atelier and as a driving force in the public life of the Hungarian architects has been studied intensively, to his work as professor at the Joseph Technical University of Budapest has been so far less attention given. Steindl began to teach as an ordinary professor of medieval architecture in 1870 and shaped the curriculum and educational methods following the traditions of his former alma mater, the Academy Fine Arts of Vienna. In this study, beyond the outline of the long 19th-century Hungarian architectural education and analysis the educational principals typical of Steindl’s methods, the manuscript of the professor’s lecture notes is published and analyzed, with special regard to his historiographical orientation and scholarly reference points. The philological reading of the text points out, that Steindl compiled his lectures in question from the ‘great syntheses’ of the Berlin School of Art History, above all that of Wilhelm Lübke and Karl Schnaase. The detection of this kind of historiographical influence may contribute to the scholarship’s image of Steindl’s, furthermore the late 19th-century Hungarian architectural intelligentsia’s erudition.
.030 ± 0.001 m%). These results imply that the object is covered with corrosion products, especially the rivets and their vicinity. Table 1. PGAA results of the dagger (Inv. Nr. 2013.10.03.) from Ráksi find in mass percent (m%) unit. Sb (detection limit: 0
The Hungarian painter Lajos Vajda left little in terms of documentation for posterity. His witten legacy is largely confined to three notebooks and a variety of scribbled notes that complement the letters saved by his wife.
Despite the scarcity of documentation, the three “chequered notebooks” (named after their chequered covers) provide a considerable insight into the scope of Vajda’s reading especially when analysed in conjunction with the letters.
After initially attempting to “reconstruct” Vajda’s intellectual background through his reading, I discovered an important gap in the academic research conducted into Vajda to date.
This related to the drawings of houses and streets in the small town of Szentendre that Vajda produced between 1935 and 1938 – contemporaneously with the writing of the Chequered Notebooks. These drawings have largely been seen as part of a larger project. However, my reading of these notes leads me to believe that they were selfstanding experiments in the depiction of space influenced by his concurrent reading and reflection on Egyptian and Persian ideas of the depiction of space.
Of especial interest is the role of Vajda’s line drawings and the possibility of exchanging positive and negative forms. This problematic had already appeared in his earlier montage works but – despite the similarities in style – he now experimented with new solutions to the depiction of space.
The shapes do not mark overlapping layers of space but rather they enclose a special form through distortions projected onto the plane. Vajda did not use the usual systems of geometrical depiction (the Monge type depiction system, axonometric or one and two-point directional perspective) or depict the motifs from a single point of view, but drew the contours by following the detection and the direction of the gaze.
In summary, my research aimed at discovering the extent to which knowledge of the artist’s reading material assists in the analysis of works created at the same time. In the comparison with the experiments in the depiction of space of that era, I was interested in how Vajda’s experiments with space were different and what relationship they had with his reading material (Egyptian, Persian Art).
. Forschungen in Tell-Siedlungen an Donau und Theiß. Budapest.
Menze, B. H.–Ur, J. A.–Sherratt, A. G. 2006 Detection of Ancient Settlement Mounds: Archaeological Survey Based on the SRTM Terrain Model. Photogrammetric Engineering
(‘music’), while lágy (‘soft’) and puha (‘tender’) are synonyms in the context of margarin (‘margarine’) and fém (‘metal’). Our main objective in the present proof-of concept experiment is the detection of adjectival cliques of this type: cliques
. This also highlights a potential limitation when using screen recording in and of itself as a diagnostic protocol for error detection in this context. We can readily see and articulate on errors in conjunction with on-screen phenomena that occur prior