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, 2009; Becker et al., 2014 ). Similar to the emergence of polymerase chain reaction, the backbone of modern molecular biology, matrix-assisted laser desorption ionisation–time of flight mass spectrometry (MALDI-TOF MS) has also become a paradigm
uropathogens in urine by MALDI-TOF MS can significantly shorten the identification time from 24 to 48 h, using classical methods, to 30 min [ 7, 8 ]. In our study, our aim was to detect positive urine samples by flow cytometry and to identify bacteria directly
We report the case of a nosocomial infection due to Enterococcus cecorum isolated from a blood culture of a 75-year-old septic male patient. Matrix-assisted laser desorption–ionization time-of-flight mass spectrometry (MALDI-TOF MS) and Vitek 2 succeeded in identification of the isolate.
molecular biology techniques (Agius et al., 2007). Matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI- TOF MS) allows the identification of microorganisms isolated from food or clinical cases by a low-cost, rapid, easy
Introduction The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) provides a fast, simple and cost-effective diagnosis in clinical microbiology laboratories, which is increasingly found a place in the
Members of the genus Bacteroides are important components of the normal microbiota of gastrointestinal tract; however, as opportunistic pathogens are also associated with severe or even life-threatening infections with significant mortality. Various species within Bacteroides fragilis group are phenotypically very similar; thus, their identifications with traditional-automated biochemical methods are frequently inaccurate. The identification of the newly discovered or reclassified bacteria can be doubtful because of the lack of biochemical profile in the database of these tests. The aim of this study was to determine the accuracy of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) method by testing of 400 Hungarian Bacteroides clinical isolates. Inaccurate identification results with MALDI-TOF MS were confirmed by 16S rRNA gene sequencing and findings were compared with traditional-automated biochemical test rapid ID 32A method as well.
Liu, L., Wang, A., Appels, R., Ma, J., Xia, X., Lan, P., He, Z., Bekes, F., Yan, Y., Ma, W. 2009. A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding. J. Cereal Sci. 50 :295
Lipids are important natural products and essential in nutrition, cosmetic formulations, pharmaceuticals, etc. Lipids and, particularly, phospholipids are of substantial medical interest (some are molecules with messenger function) and of diagnostic potential (for instance, the lipoproteins in human blood). Among the different soft-ionization mass spectrometric methods that enable detection of the intact lipid molecules, matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has several advantages, for instance, simple performance, high sensitivity, and robustness against contaminants. Additionally, MALDI-TOF MS analyzes a solid sample. This enables (in contrast with isotropic solutions) acquisition of spatially-resolved mass spectra (‘mass spectrometric imaging’). However, separation of complex mixtures into the individual lipid classes is normally required to enable detection of all the components. It will be shown with the example of a lipid extract from hens’ egg yolk that MALDI-TOF MS can be easily combined with TLC, enabling detection of as little as picomole amounts of lipids directly on the HPTLC plate. This results in sensitivities higher than those from established staining procedures. Additionally, because of the substantial spatial resolution, lipids separated by normal-phase TLC may not only be differentiated according to differences of their headgroups but also according to differences of their fatty acyl composition. Finally, MS-MS experiments, providing further insights into the structures of the relevant lipids, can be also performed directly on the HPTLC plate. Although the HPTLC-MALDI coupling can be easily established, there are different points to which special attention should be paid. Aspects of matrix application, data acquisition (including the stability of lipids and reproducibility), and data evaluation will be emphasized in this paper
]. Nevertheless, the introduction of matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) has represented a technological revolution in clinical microbiological diagnostics [ 32, 33 ]. This technology allows for protein
A total of 232 accessions of tetraploid species, durum wheat (Triticum turgidum L. ssp. durum Desf., 2n=4x=28, AABB) with a widespread origin of various countries were used in this study. Their high molecular weight glutenin subunit (HMW-GS) composition was identified by Matrix-assisted laser desorption/ionization time-of-flight Mass Spectrometry (MALDI-TOF-MS). Among all accessions analyzed, 194 were homogeneous for HMW-GS, 38 were heterogeneous, and 62 possessed unusual or new subunits. The results revealed a total of 43 alleles, including 5 at Glu-A1 and 38 at Glu-B1, resulting in 60 different allele combinations. The Glu-B1 locus displayed higher variation compared with Glu-A1. Glu-A1c (55.2%) and Glu-B1aj (17.7%) were the most frequent alleles at Glu-A1 and Glu-B1, respectively. Two allele types (“null” and 1) at the Glu-A1 locus and three allele types (7OE + 8, 14+15, 8) at the Glu-B1 locus appeared to be the common types in the 232 accessions. A total of 23 new alleles represented by unusual subunits were detected at the Glu-A1 and the Glu-B1 locus.
