Search Results
, 2009; Becker et al., 2014 ). Similar to the emergence of polymerase chain reaction, the backbone of modern molecular biology, matrix-assisted laser desorption ionisation–time of flight mass spectrometry (MALDI-TOF MS) has also become a paradigm
We report the case of a nosocomial infection due to Enterococcus cecorum isolated from a blood culture of a 75-year-old septic male patient. Matrix-assisted laser desorption–ionization time-of-flight mass spectrometry (MALDI-TOF MS) and Vitek 2 succeeded in identification of the isolate.
molecular biology techniques (Agius et al., 2007). Matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI- TOF MS) allows the identification of microorganisms isolated from food or clinical cases by a low-cost, rapid, easy
Liu, L., Wang, A., Appels, R., Ma, J., Xia, X., Lan, P., He, Z., Bekes, F., Yan, Y., Ma, W. 2009. A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding. J. Cereal Sci. 50 :295
]. Nevertheless, the introduction of matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) has represented a technological revolution in clinical microbiological diagnostics [ 32, 33 ]. This technology allows for protein
A total of 232 accessions of tetraploid species, durum wheat (Triticum turgidum L. ssp. durum Desf., 2n=4x=28, AABB) with a widespread origin of various countries were used in this study. Their high molecular weight glutenin subunit (HMW-GS) composition was identified by Matrix-assisted laser desorption/ionization time-of-flight Mass Spectrometry (MALDI-TOF-MS). Among all accessions analyzed, 194 were homogeneous for HMW-GS, 38 were heterogeneous, and 62 possessed unusual or new subunits. The results revealed a total of 43 alleles, including 5 at Glu-A1 and 38 at Glu-B1, resulting in 60 different allele combinations. The Glu-B1 locus displayed higher variation compared with Glu-A1. Glu-A1c (55.2%) and Glu-B1aj (17.7%) were the most frequent alleles at Glu-A1 and Glu-B1, respectively. Two allele types (“null” and 1) at the Glu-A1 locus and three allele types (7OE + 8, 14+15, 8) at the Glu-B1 locus appeared to be the common types in the 232 accessions. A total of 23 new alleles represented by unusual subunits were detected at the Glu-A1 and the Glu-B1 locus.
Waterbloom samples of Microcystis aeruginosa and Planktothrix agardhii were collected from a variety of ponds, lakes and reservoirs in Hungary. Samples were tested with matrix-assisted laser desorption/ionization — time-of-flight mass spectrometry (MALDI-TOF MS) to identify the microcystin forms. The concentration of the microcystins was measured with capillary electrophoresis and the toxicity was tested by sinapis test. DNA was extracted from the samples and tested using a range of primers linked to the biosynthesis of microcystin. All of the fourteen collected samples gave positive results for the presence of the mcy genes with PCR products with sizes between of 425 and 955 bp, respectively, indicating the presence of the genes implicated in the production of microcystins. The results showed that a wide range of microcystin (MC) forms were detected in the Microcystis containing samples, among which MC-LR, -RR, and -YR were the most common. The highest MC concentration was 15,701 mg g−1, which was detected in an angling pond. The samples containing Planktothrix agardhii were less toxic, and the most common form in this species was the Asp3-MC-LR.
.H., Xia, X.C., He, Z.H., Yan, Y.M. 2008a. Characterization of HMW glutenin subunits in common heat and related species by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF-MS). J. Cereal Sci. 47 :252
much faster than two-dimensional gel electrophoresis (2D GE) and able to catch low molecular weight stress proteins, Matrix-Assisted Laser Desorption Ionization - Time of Flight Mass Spectrometry (MALDI-TOF MS) protein profiling also makes it possible
-assisted laser desorption ionization time-of-flight (MALDI-TOF)mass spectrometer. Comparison with routine methods used in clinical microbiology laboratories Enferm Infecc Microbiol Clin 28 492 – 497
