Search Results

You are looking at 1 - 10 of 523 items for :

  • Biology and Life Sciences x
  • Refine by Access: All Content x
Clear All

Edwards, S.G., O’Callaghan, J., Dobson, D.W. 2002. PCR-based detection and quantification of mycotoxigenic fungi. Mycological Research 106 :1005–1025. Dobson D.W. PCR

Restricted access

-specific PCR-based assays for the detection of Fusarium species and a comparison with the whole seed agar plate method and trichothecene analysis. International Journal of Food Microbiology 103 :271–284. Gaba

Restricted access

the spread of MRSA and SA [ 6 , 7 ]. The German MRSA screening guidelines [ 8 ] recommend culture-based methods as the basis of MRSA screening. Due to the high negative predictive value (NPV) and rapid turnaround time, PCR-based methods are

Open access

, Jung M , Meller S , Kristiansen G Improved PCR performance using template DNA from formalin-fixed and paraffin-embedded tissues by overcoming PCR inhibition . PLoS One 8 , 1 – 10 ( 2013 ) 3

Open access

, Thomson RB Jr. , Peterson LR , Kaul KL : Real-time PCR for detection and identification of Plasmodium spp . J Clin Microbiol 43 , 2435 – 2440 ( 2005 ) 8. Berry

Open access

biopsies. If only cysts are seen, discrimination of E. histolytica from non-pathogenic species can be performed either by PCR or antigen testing [ 2 ]. While diagnostic accuracy of modern real-time PCR for the detection of E. histolytica in stool

Open access
European Journal of Microbiology and Immunology
Authors:
Alexis Lacout
,
Marie Mas
,
Julie Pajaud
,
Véronique Perronne
,
Yannick Lequette
,
Michel Franck
, and
Christian Perronne

patients suffering from polymorphic signs and symptoms (SPPT/PTLDS), using real time qPCR, which is a direct diagnostic method amplifying the DNA of the microorganisms sought. As the yield of PCRs looking for Borrelia or co-infections in the venous blood is

Open access

. Pierce VM , Elkan M , Leet M , McGowan KL , Hodinka RL : Comparison of the Idaho Technology FilmArray system to real-time PCR for detection of respiratory pathogens in children . J Clin Microbiol 50 , 364 – 371 ( 2012

Open access

Enniatins (ENs), produced by Fusarium species are a group of mycotoxins with antimicrobial, insecticidal (GROVE & POPLE, 1980) and phytotoxic activities. PCR based assays were applied for detecting enniatin-producing strains of Fusarium avenaceum, F. poae and F. sporotrichioides isolated from wheat seeds originated of 30 geographic localities of Hungary. All F. sporotrichoides strains and except two of all F. poae strains gave positive signal to esysp1 and esysp2 primers as well as all F. avenaceum isolates were positive to esya1 and esya2 primers indicating the ability to produce ENs. This is a first report of the enniatin producing ability of Fusarium species associated to wheat in Hungary.

Restricted access

Campylobacter jejuni and C. coli are among the most important causes of acute diarrhoea in humans throughout the world. Poultry meat is a major source of Campylobacter infections. Sensitive detection methods are necessary to identify contaminated samples. Detection of campylobacters by culturing is slow and tedious, whereas PCR technology offers the potential for rapid and sensitive detection, however, it may be inhibited when used directly for food or pre-enriched food samples. Different methods for sample and/or DNA preparation were studied to find an optimal combination for sensitive PCR detection of C. coli in enrichment broth. Buoyant density centrifugation (BDC) prior to cell lysis improved PCR detection of C. coli by 100-1000-fold. Preston enrichment broth spiked with 101-102 CFU ml-1 was detected positive after 18 h of enrichment. Specific flaA PCR detection of C. coli in enrichment broth following BDC and simple heat lysis of the cells can be conducted within two working days. This study is a part of the undergoing development of a rapid and sensitive molecular procedure for specific detection of C. coli in foods.

Restricted access