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Abstract
A thermodynamic study on the interaction of bovine carbonic anhydrase II (CAII) with nickel ions was performed by using isothermal titration calorimetry (ITC) at 27 °C in Tris buffer solution at pH = 7.5. The enthalpies of Ni2+ + CAII interaction are reported and analysed in terms of the new solvation theory. It was indicated that there are three identical and non-cooperative sites for Ni2+. The binding of a nickle ion is exothermic with dissociation equilibrium constants of 81.306 and 99.126 μM at 27°C and 37°C, respectively. The binding of nickel ions can cause some changes in the stability of the enzyme at low and high Ni2+ concentrations.
above-mentioned target protein belongs to the amine oxidoreductase and carbonic anhydrase target class and has a wide range of research. For example, monoamine oxidase B (MAOB) is the enzyme that regulates the degradation of dopamine [ 13 ] and plays a
Abstract
A novel method for the characterization of metal containing biological compounds has been developed which utilizes both nuclear activation [e.g.,12C(3He, α)11C] and atomic activations (X-ray emission) induced by 8 MeV3He bombardment. Detection limits for carbon (≈1 μg), oxygen (≈1μg), and metals (1 to 10 ng for elements between P and Zn) were obtained under routine experimental conditions. The metal stoichiometry of six different compounds (alkaline phosphatase, amylase, carbonic anhydrase, diamine oxidase, my oglobin, vitamin B12) were determined with typical relative precisions of ±25% for a 40 μg sample. A comparison of these ratios with carbon and metal measurements obtained by other methods showed a relative accuracy of 1 to 20%.
) by carbonic anhydrase [ 26 ]. Freshwater alga Chlorella grows on the mineral environments which do not contain NaCl. At low concentration of NaCl the pH is shift in growing Chlorella culture a little raised during the first 20–25 min, then
thermodynamic study on the binding of cobalt and iron ions with bovine carbonic anhydrase II molecule at different temperatures . J Solut Chem . 2010 ; 39 : 1142 – 115214 . 10.1007/s10953-010-9572-3 . 12
inhibition of salicylic acid derivatives on human cytosolic carbonic anhydrase isozymes I and II . Bioorg Med Chem 2008 16 : 9101 – 9105 10.1016/j.bmc.2008.09.028 . 5. Andréa V
