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Experiments were carried out on sperm cryopreservation of two European percid fish species, the pikeperch (Sander lucioperca) and the Volga pikeperch (S. volgensis) . Two experiments were conducted on pikeperch sperm. In the first, the effects of three extenders (Glucose, KCl, Sucrose) and two cryoprotectants (dimethyl-sulfoxide: DMSO, methanol: MeOH) were tested on motility and fertilization. In the second, the effects of two dilution ratios (1: 1, 1: 9) and two cryoprotectants (DMSO, MeOH) on hatching were investigated. In the experiment on Volga pikeperch the suitability of using cryopreservation for fertilization was investigated. In the first experiment on pikeperch the highest post-thaw motility (28 ± 21%) and fertilization rate (43 ± 12%) was found with DMSO as cryoprotectant in combination with Glucose extender. In the second, the highest hatch rate (41 ± 22%) was observed with MeOH as cryoprotectant and 1: 1 sperm dilution ratio, however no significant difference was found among the results. In the experiment on Volga pikeperch hatch rates with cryopreserved sperm (60 ± 2%) did not significantly differ from the control (60 ± 6%). Contamination of sperm with urine seems to be a key problem in the success of sperm cryopreservation of these species.

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. (2002) Behavioural assessment of dimethylsulfoxide neurotoxicity in rats. Toxicol. Lett. 132 , 117–121. Coudoré F. Behavioural assessment of dimethylsulfoxide neurotoxicity in

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Bruck, R., Aeed, H., Shirin, H., Matas, Z., Azidel, L., Avni, Y. and Halpern, Z. (1999): The hydroxyl radical scavengers dimethylsulfoxide and dimethylthiourea protect rats against thioacetamide-induced fulminant

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. (1980): Effects of sodium butyrate and dimethylsulfoxide on biochemical properties of human colon cancer cells. Cancer , 5 , 1185-1192. Effects of sodium butyrate and dimethylsulfoxide on biochemical properties of human colon

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Kim YS, Tsao D, Siddiqui B, Whitehead JS, Arnstein P, Bennett J, Hicks J: Effects of sodium butyrate and dimethylsulfoxide on biochemical properties of human colon cancer cells. Cancer 45, 1185–1192 (1980

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