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adhesion to fibronectin (CadF) [ 23 , 24 ], major outer membrane protein (MOMP) [ 25 ], periplasmic binding protein (PEB1) [ 26 ], P95 [ 27 ], jejuni lipoprotein A (JlpA) [ 28 , 29 ], Campylobacter autotransporter protein A (CapA) [ 30 ], and
, E. , Kometani , M. , Tozaki-Saitoh , H. , Inoue , K. ( 2009 ) Mechanisms underlying fibronectin-induced up-regulation of P2X4R expression in microglia: distinct roles of PI3K-Akt and MEK-ERK signalling pathways . J. Cell. Mol. Med. 13
atmosphere. The following day, potential growth inhibition zones around the discs were scrutinized by macroscopic inspection. For assessment of biofilm formation, experiments were performed in 96-well microtiter plates coated with human fibronectin
., Bernardo-Filho, M., Meirelles, M. N., Tournier, J. M., Puchelle, E. (1993) Pseudomonas aeruginosa binds to soluble cellular fibronectin. Current Microbiol. 26 , 91-95. Current Microbiol
Arencibia I , Suarez NC , Wolf-Watz H , Sundqvist KG . Yersinia invasin, a bacterial beta1-integrin ligand, is a potent inducer of lymphocyte motility and migration to collagen type IV and fibronectin . J Immunol . 1997
Using quantitative phosphopeptide sequencing of unstimulated versus stimulated primary murine Foxp3+ regulatory and Foxp3− conventional T cells (Tregs and Tconv, respectively), we detected a novel and differentially regulated tyrosine phosphorylation site within the C1 domain of the guanine-nucleotide exchange factor CalDAG GEFI. We hypothesized that the Treg-specific and activation-dependent reduced phosphorylation at Y523 allows binding of CalDAG GEFI to diacylglycerol, thereby impacting the formation of a Treg-specific immunological synapse. However, diacylglycerol binding assays of phosphomutant C1 domains of CalDAG GEFI could not confirm this hypothesis. Moreover, CalDAG GEFI−/− mice displayed normal Treg numbers in thymus and secondary lymphoid organs, and CalDAG GEFI−/− Tregs showed unaltered in vitro suppressive capacity when compared to CalDAG GEFI+/+ Tregs. Interestingly, when tested in vivo, CalDAG GEFI−/− Tregs displayed a slightly reduced suppressive ability in the transfer colitis model when compared to CalDAG GEFI+/+ Tregs. Additionally, CRISPR-Cas9-generated CalDAG GEFI−/− Jurkat T cell clones showed reduced adhesion to ICAM-1 and fibronectin when compared to CalDAG GEFI-competent Jurkat T cells. Therefore, we speculate that deficiency in CalDAG GEFI impairs adherence of Tregs to antigen-presenting cells, thereby impeding formation of a fully functional immunological synapse, which finally results in a reduced suppressive potential.
C.R. Young 1999 The absence of cecal colonization of chicks by a mutant of Campylobacter jejuni not expressing bacterial fibronectin-binding protein Avian Dis
931 Euker TP, Konkel ME: The cooperative action of bacterial fibronectin-binding proteins and secreted proteins promote maximal Campylobacter jejuni invasion of host cells by stimulating
ME Konkel 2012 The cooperative action of bacterial fibronectin-binding proteins and secreted proteins promote maximal Campylobacter jejuni invasion of host cells by stimulating membrane ruffling
.M. Overall 1998 The involvement of the fibronectin type II-like modules of human gelatinase A in cell surface localization and activation J Biol Chem 273 32