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Cereal Research Communications
Authors: Tibor Bartók, András Szekeres, Árpád Szécsi, Mihály Bartók, and Ákos Mesterházy

Fumonisins were extracted from a rice culture which had been inoculated with Fusarium verticillioides isolated from maize stalk. The culture extracts were analysed by a hyphenated technique: reversed-phase high-performance liquid chromatography/electrospray ionization ion-trap mass spectrometry (RP-HPLC/ESI-IT MS 2 ). Besides already known fumonisins, a new type of fumonisin series (PHFB4OSA belonging to the formerly described FBX)-containing four new compounds-was detected. At these compounds, one of the OH groups attached to the fumonisin backbone was esterified by carboxylic acid (oxalylsuccinic acid, OSA) other than tricarballylic acid (TCA). The masses of the protonated molecules and of the characteristic product ions and the characteristic neutral mass losses from the protonated molecules suggested their structure. The relative quantities of the new minor compounds were expressed as percentages of FB1 toxin.

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This study investigated the chemical properties of Durio zibethinus (Durian) in Malaysia. The cultivars included in the present study were D101, D197, and Kampung. A wide range of chemical properties such as proximate nutritional content, minerals, total phenolic content was analysed using established analytical methods. The antioxidant capacity in term of free radical scavenging activity was determined using colorimetric assay, whereas metabolite profiles of samples were analysed by hyphenated high throughput tool like GC-MS. The results showed that D197 durian had the highest content of ash, protein, fat, and carbohydrate, but the lowest moisture level. Potassium was found to be the highest mineral (8.68–11.36 mg g–1), followed by sodium (3.2–7.6 mg g–1), magnesium (0.86–1.88 mg g–1), and calcium (2.74–3.80 mg g–1) in the tested cultivars. The major sulphur containing compounds (% relative peak area) such as hydrogen sulphide (5.0–8.3%), methanethiol (0.5–1.0%), ethanethiol (20.7–35.0%), 1-propanethiol (2.7– 9.5%), and diethyl disulphide (0.6–1.9%), and esters, especially ethyl 2-methylbutanoate (10.0–15.0%), methyl 2-methylbutanoate (1.0%), and propyl 2-methylbutanoate (1.5%) were detected when fresh samples were heated in headspace GC-MS. However, those compounds evaporated during ultrasound assisted extraction and oven drying even at 40 °C.

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-Carretero , A. , Menéndez , J.A. , Menéndez-Gutiérrez , M.P. , … & Fernández-Gutiérrez , A. ( 2010 ): Qualitative screening of phenolic compounds in olive leaf extracts by hyphenated liquid chromatography and preliminary evaluation of cytotoxic activity

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., Rode, J., 1998: Determination of rutin in buckwheat leaves. Chromatography and Hyphenated Techniques. Bled. 153. Kim, J.S., Park, Y. J., Yang, M.H., Shim, J.W., 1994: Variation of rutin content in seed and plant of

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1 11 Lommen, A. (2009): MetAlign: interface-driven, versatile metabolomics tool for hyphenated full-scan mass spectrometry data preprocessing. Anal. Chem. , 81

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