Authors:G. Péter, D. Dlauchy, E. Szűcs, and J. Tornai-Lehoczki
In this study a simple and effective method was developed for the isolation of Saccharomyces strains from grapes. Aseptically collected grape samples were processed by enrichment in a nutritive basal medium supplemented with 10% (v/v) methanol followed by isolation of yeast strains. Sixteen of the 18 grape samples yielded Saccharomyces strain(s). More than 70% of the isolates belonged to the genus Saccharomyces. Based on phenotype and electrophoretic karyotyping, all strains of Saccharomyces were identified as S. cerevisiae. For several grape samples, varying physiological characters, the number of spores per asci, and the observed chromosome length polymorphisms provided evidence for diversity of S. cerevisiae strains obtained by this enrichment in methanol-containing broth. Results indicated that enrichment in methanol-containing broth is an effective alternative method to facilitate isolation of Saccharomyces strains from grapes. The enrichment method described in this work provides a simple and effective tool for isolation of Saccharomyces strains from grapes. The method may be applied in studying wine fermentation ecology, as well as for the isolation of potential starter strains from grapes.
Authors:B. Kupcsulik, B. Sevella, A. Ballagi, and J. Kozma
, H. & Delgado, J. M. (1997): Different methanol feeding strategies to recombinant Pichia pastoris cultures producing high level of dextranase. Biotech. Tech. , 11 , 461-466.
Different methanol feeding strategies to
Authors:Nada Žnidaršič, Polona Mrak, Eva Rajh, Kristina Žagar Soderžnik, Miran Čeh, and Jasna Štrus
acetone, and embedding in Agar 100 epoxy resin; (2) overnight fixation in absolute methanol and direct embedding in Agar 100 epoxy resin.
Labeling with CBP-FITC was performed on 7 μm paraffin sections and on 0.5 μm semithin sections, prepared as
Authors:Siddavatam Naresh Kumar Reddy and Mohmad Marouf Wani
converted to palm oil methyl ester (POME) using an alkaline transesterification process. Methoxide solution was made by a 1% volume of sodium hydroxide dissolved in 25% volume of methanol with a magnetic stirrer. Raw palm oil was heated up to 65 °C using an
Authors:B. Keramane, N. Touati, K. Saidani, A. Taguelmimt, I. Zair, and F. Bedjou
bioactive molecules has become a necessity. Thus, the aim of this work was to evaluate antioxidant activity of four diﬀerent extracts (water, ethanol, methanol, and chloroform) of two brown algae Padina pavonica and Cystoseira mediterranea from the west
Authors:F.S. Mohammed, M. Pehlivan, E. Sevindik, H. Akgul, M. Sevindik, I. Bozgeyik, and O. Yumrutas
dried plant samples were powdered using a mechanical grinder. Extraction with methanol (MeOH) and dichloromethane (DCM) was performed with 250 mL at 50 °C for 6 h using 30 g of powdered plant samples. Crude extracts of plants were obtained by removing
Authors:G. Korekar, T. Stobdan, H. Singh, O. Chaurasia, and S. Singh
The outcome of various solvent extraction (water, methanol, acidic 50% methanol, 70% acetone, acidic 50% methanol followed by 70% acetone) on the total phenolic content (TPC) and antioxidant capacity of fruit pulp, seeds, leaves and stem bark of seabuckthorn (Hippophae rhamnoides L.) was investigated. The seabuckthorn extracts possess high phenolic content, 1666–13769 mg GAE/100 g d.w. The mean TPC was found highest in seeds (11148) followed by stem bark (10469), leaves (6330) and pulp (3579 mg GAE/100 g d.w.). In general, the 70% acetone and acidic 50% methanol followed by 70% acetone extracts was found to contain significantly higher TPC than those obtained in other extracting solvents. Antioxidant capacity in terms of IC50 value of pulp (3.39 mg ml−1) was up to 7.8 times higher than those reported for stem bark (0.43 mg ml−1) and up to 2.4 times higher than those found in seeds (1.4 mg ml−1). Further, antioxidant capacity by FRAP assay showed that the stem bark possess maximum antioxidant capacity (16.83) followed by seeds (15.26), leaves (12.73) and pulp (12.61), all as mM FeSO4. Significant correlation was found between TPC and antioxidant capacity by DPPH and FRAP assays.