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Progress in Agricultural Engineering Sciences
Authors: Szilvia Bánvölgyi, Eszter Dusza, Fiina K. Namukwambi, István Kiss, Éva Stefanovits-Bányai, and Gyula Vatai

–Ciocalteu reagent was purchased from Merck KGaA. (Darmstadt, Germany), the gallic acid was obtained from Sigma-Aldrich, Chemicals Company (St. Louis, MO, USA). The methanol and sodium carbonate were purchased from Reanal Laborvegyszer Ltd. (Budapest, Hungary

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determined according to the Folin–Ciocalteu colorimetric method as described by Singleton and Rossi (1965) . Briefly, 1,250 µL of Folin solution (1:10 v/v Folin; distilled water) was added in the test tube followed by addition of 150 µL of methanol (4:1 v

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) , betacyanin is more tolerant to reduce color than betaxanthin during storage. Conventional solid-liquid extraction is based on solvation of solid materials with proper portion of solvent under heating or agitation. Herein, water, methanol-water, and ethanol

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dissolved in isooctane, and ester derivative was prepared with 2M KOH in methanol and neutralized with NaHSO 4 . The analysis of fatty acid methyl-esters were performed by GC-MS (Shimadzu, 2010; Supleco-wax column). FAME-mix and Grain-mix standards were used

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