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is not easily demonstrated. A recent publication was able to demonstrate colibactin activity by means of the Comet Assay after exposing HeLa cells to a pks -positive E. coli strain, but only when the cells were additionally treated by 8 Gy gamma
) and gas-liquid chromatography (GLC) [ 19, 98, 99 ]. These methods were quite pricy, were available in only a few diagnostic laboratories and provided identification results only 48–72 hours later, mostly on the genus level [ 19, 98, 99 ]. Recent
Numerous recent publications have compared different cultivars of broccoli and different cultivation conditions suggesting that great variations in the content of phytochemicals among cultivars are due to genetic, and many environmental factors. The purpose of the present work was the quantification of glucosinolates, phenolic compounds, carotenoids, and antioxidant capacity in the florescences of 13 genotypes from freshly harvested broccoli grown under uniform agroecological practices. The present results showed a significant variation in the level of bioactive compounds and consequently potential health benefits of broccoli (total glucosinolates 12.04–22.48 μmol g−1 d.w., the total phenolic content 15.54–26.92 mg g−1 d.w., total carotenoid content 0.19 mg g−1–0.46 mg g−1 d.w.). Studied cultivars were different in the level of the individual bioactive compounds, although some cultivars had higher content of all three analysed bioactive compounds such as Marathon, Ironman and General cultivar. In addition, these three cultivars showed excellent marketable properties and we choose them for the further breeding program in order to study variation among bioactive compounds in more details.
this intention. We further conclude that surrogate endpoints of the second and third category cannot be predictive by themselves because they are not sufficient for the endpoint of interest. Therefore, it is not surprising that the authors of a recent
