Authors:A. Özer, Selcan Turker, Seyda Çolak, Mustafa Korkmaz, Ekrem Kiliç, and Meral Özalp
The use of gamma rays for the sterilization of pharmaceutical raw materials and dosage forms is an alternative method for sterilization. However, one of the major problems of the radiosterilization is the production of new radiolytic products during the irradiation process. Therefore, the principal problem in radiosterilization is to determine and to characterize these physical and chemical changes originating from high-energy radiation. Parenteral drug delivery systems were prepared and in vitro characterization, biodistribution and treatment studies were done in our previous studies. Drug delivery systems (liposomes, niosomes, lipogelosomes and niogelosomes) encapsulating diclofenac sodium (DFNa) were prepared for the treatment of rheumatoid arthritis (RA). This work complies information about the studies developed in order to find out if gamma radiation could be applied as a sterilization method to DFNa, and the raw materials as dimyristoyl phosphatidylcholine (DMPC), surfactant I [polyglyceryl-3-cethyl ether (SUR I)], dicethyl phosphate (DCP) and cholesterol (CHOL) that are used to prepare those systems. The raw materials were irradiated with different radiation doses (5, 10, 25 and 50 kGy) and physicochemical changes (organoleptic properties pH, UV and melting point), microbiological evaluation [sterility assurance level (SAL), sterility and pyrogen test] and electron spin resonance (ESR) characteristics were studied at normal (25 °C, 60% relative humidity) and accelerated (40 °C, 75% relative humidity) stability test conditions.
A szárazsó-belégzés a sóbányák egészségre hasznos klímáját reprodukálja
mesterséges környezetben. Lényege, hogy a vegytiszta nátrium-klorid-kristályokat
3 µm-nél kisebb átmérőjű szemcsékké őrlik és elporlasztva egy kellemesen hűvös
és alacsony relatív páratartalmú szoba levegőjébe fújják, amíg annak
koncentrációja 10–30 mg/m3 lesz a szoba levegőjében. A beteg (vagy
egészséges) személyek ezt lélegzik be 30–60 percen keresztül, általában
kúraszerűen 10–20 egymás utáni alkalommal. A belélegzett só ozmózisos nyomása
révén csökkenti a nyálkahártya-ödémát, mérsékli annak gyulladását, hígabbá teszi
a váladékot, könnyíti és gyorsítja annak kiürítését (ezáltal a légszennyező
anyagok és allergének eltávolítását is), gátolja a baktériumok növekedését, sőt
elpusztítja azok egy részét és fokozza a fagocitaaktivitást. Kedvező hatással
van a betegek közérzetére (relaxációs hatású), megelőzi vagy legalább ritkábbá
teszi a légúti gyulladásokat, sőt a kóros légzésfunkciós értékeket is javítja.
Jelentősen mérsékli a hörgő-hiperreaktivitás mértékét, ami a gyulladás
csökkenésére utal. Nem csupán az alsó légúti gyulladások kezelésére alkalmas,
hanem a heveny és idült felső légútiakéra is. Kedvező hatása néhány idült
bőrbetegségben (például atopiás dermatitis, psoriasis, pyoderma) is bizonyított.
E kezelés (Indisó néven) orvosi ellenőrzés mellett Magyarországon is
hozzáférhető. Orv. Hetil., 2015, 156(41), 1643–1652.
Authors:Andrea Kőrösi Molnár, László Kőrösi, Bence Balázs, and András Gáspárdy
. The chickens were exposed to heat challenge twice: on day 19 (31 °C, 83% relativehumidity, RH/8 h, the heat index was 42 °C) and on day 35 (32 °C, 82% RH/10 h, the heat index was 42 °C). Body weight The birds were weighed individually every week. Body
Authors:Lydia Gil, S. Saura, Arantxa Echegaray, Felisa Martinez, I. de Blas, A. Akourki, Noelia Gonzalez, E. Espinosa, and A. Josa
The present study evaluated the effect of supplementing the medium used to mature equine oocytes in vitro with oestrous mare serum (EMS) or horse follicular fluid (HFF). To this end, 144 ovaries were obtained from mares aged 16-21 months and transported to the laboratory in Dulbecco's phosphate buffered saline (D-PBS) at 30°C. Oocytes were harvested from the ovaries by slicing, and then selected for in vitro maturation (IVM) according to the number of cumulus cell layers and the characteristics of the cytoplasm. The selected oocytes were washed three times in TCM199 medium plus HEPES (TCM-199H) or in the same medium plus glutamine (TCM-199G), then matured in vitro in six study groups established according to the in vitro maturation (IVM) treatment to see possible interactions between HEPES and glutamine on other supplements: Ten percent EMS was added to two of these media (TCM-199H+EMS and TCM-199G+EMS) and 10% HFF was added to the media in two other groups (TCM-199H+HFF and TCM-199G+HFF). IVM was performed at 38.5°C for 40 h in a controlled atmosphere (5% CO2, 95% relative humidity). The findings indicate that the presence of EMS or HFF in the TCM-199H medium gives rise to the best results in terms of the proportions of oocytes reaching maturity (37.7% and 36.8%, respectively). The values obtained with EMS and HFF were statistically similar to each other but differed from the other treatments. The media containing glutamine led to the highest proportions of degenerated oocytes.
Authors:Inmaculada Cuevas, Alfonso Carbonero, David Cano, Isabel L. Pacheco, Juan C. Marín, and Carmen Borge
., 2011 ), the most affected animals were young calves and the average relativehumidity recorded in the month of disease onset was 72.9%. Although mortality is nearly 100% when HS is first introduced in an area ( De Alwis, 1992 ), in this study both
-Dawley virgin rat dams (Charles River Laboratories, Prague, Czech Republic) were mated at 10 weeks of age and individually housed in plexiglass cages in a temperature-controlled environment of 22 ± 2 °C with a relativehumidity of 55 ± 10% and 12 h light
Authors:Katiuska Satué, Esterina Fazio, Cristina Cravana, Marco Quartuccio, Maria Marcilla, and Pietro Medica
fresh water. The study was performed in the northern hemisphere in the months of April and May 2018 of the breeding season, to ensure the cyclic activity of the ovaries. The environmental temperature ranged between 27 and 31 °C, with 40–60% of relative
Authors:Branislav Kureljušić, Božidar Savić, Vesna Milićević, Nemanja Jezdimirović, Oliver Radanović, Jadranka Žutić, and Christiane Weissenbacher-Lang
85%, whereas ammonia concentration from 1.4 to 4 ppm. The recommended relativehumidity level in swine production facilities is between 60 and 80%, whereas the most commonly recommended threshold level for ammonia is 20 ppm. Anamnestic and clinical
Authors:Bülent Bayraktar, Emre Tekce, Hacer Kaya, Ahmet Burak Gürbüz, Ebubekir Dirican, Serol Korkmaz, Banu Atalay, and Ufuk Ülker
32–33 °C on days 0–2 then kept steady at 27–28 °C on days 3–7. From the 8th day, the stressed groups were continuously exposed to HS at 37 °C and 75–85% relativehumidity, while the stress-free control groups were held at 25 °C and 55–60% humidity
Authors:Leyla Semiha Şen, Zarife Nigar Özdemir Kumral, Gülsün Memi, Feriha Ercan, Berrak C. Yeğen, and Cumhur Yeğen
supplied by the Marmara University (MU) Animal Center (DEHAMER) and were housed in an air-conditioned room with 12-h light and dark cycles, where the temperature (22 ± 2 °C) and relativehumidity (65–70%) were kept constant. Rats were fed with standard