Authors:Özgür Appak, Selçuk Türkel, Nuran Esen, and Ayşe Aydan Özkütük
to antibiotics. Additionally, the definition is necessary to enable the collection of epidemiological data. In this study, the polymerase chain reaction (PCR)-restrictionenzyme analysis (PRA) method and DNA sequence analysis method were used to
Authors:Maryam Rahimpour Hesari, Ali Salehzadeh, and Reza Kazemi Darsanaki
Depending on 81 bp repeats, a strain analysis of PCR-RFLP products was performed with Hae III restrictionenzyme (Thermo Scientific, USA), where 10 μL of PCR product of coa gene was incubated with 6 U of the enzyme at 37 °C for 45 min
Authors:Zsuzsanna Varga, Boglárka Sellyei, Petra Paulus, Melitta Papp, Kálmán Molnár, and Csaba Székely
The objective of this study was to survey the incidence of Flavobacterium columnare in wild and cultured freshwater fish species in Hungary. This bacterium usually causes disease in waters of more than 25 °C temperature. However, with the introduction of intensive fish farming systems, infected fish exposed to stress develop disease signs also at lower temperatures; in addition, the temperature of natural waters rises to the critical level due to global warming. Twenty-five isolates from wild and cultured freshwater fishes were identified as F. columnare by specific PCR, although both the fragment lengths and the results of PCRRFLP genotyping with BsuRI (HaeIII) and RsaI restriction enzymes raised doubts regarding this species classification. Sequencing of the 16S ribosomal RNA gene revealed that 23 isolates belonged to the species F. johnsoniae and two represented Chryseobacterium spp. The isolates were found to have high-level multidrug resistance: all were resistant to ampicillin and polymyxin B, the 23 F. johnsoniae strains to cotrimoxazole, 88% of them to gentamicin, and 72% to chloramphenicol. The majority of the 25 isolates were sensitive to erythromycin (88%), furazolidone (76%), and florfenicol (68%).
Authors:Norma L. Calderón, Felipa Galindo-Mu?iz, Mireya Ortiz, B. Lomniczi, T. Fehervari, and L. H. Paasch
A Newcastle disease virus (NDV) isolated in Mexico and called Chimalhuacan strain was characterised by gene F restriction enzyme analysis and found to be a genotype II velogenic virus. Haematological evaluations and histological studies of bone marrow were conducted on chickens experimentally infected with the Chimalhuacan virus and on control chickens. Within 72 hours post infection (hpi), a 50% decrease in thrombocyte and monocyte counts and a complete cellular depletion in bone marrow islands were evident in the infected group. These findings suggest that the Chimalhuacan strain of NDV causes an early and severe damage of the haematopoietic cells including thrombocyte precursors, which might explain the marked thrombocytopenia detected in early stages of this disease.
Authors:Judit Pászti, Józsefné Király, and Miklós Füzi
Eighty isolates of Listeria monocytogenes cultured from human infections in Hungary between 2004 and 2012 were serotyped by the PCR technique of Doumith et al.  and characterised by pulsed-field gel electrophoresis (PFGE). Most of the isolates belonged to two serogroups: 53 isolates (66.3%) to serovar group 4b,4d,4e and 21 isolates (25.8%) to serogroup 1/2a,3a. Although many pulsotypes were identified a particular pulsotype proved highly excelling comprising of 31 isolates after digestion by both ApaI and AscI restriction enzymes. All strains from this pulsotype belonged to serovar group 4b,4d,4e. Interestingly 24% of isolates from invasive samples (cerebrospinal fluid, blood) belonged to two distinct pulsotypes in the less common serovar group 1/2a,3a. Several small clusters of cases caused by isolates with identical pulsotypes were identified.
Authors:K. Kamotsay, A. Herczegh, F. Rozgonyi, István Nász, Z. Gintner, and J. Bánóczy
gel electrophoresis Cell 37 67
Vazquez, J. A., Beckley, A.: Comparision of restrictionenzyme analysis versus pulsed-field gradient gel electrophoresis as a typing system for Candida albicans . J Clin Microbiol 29 , 962
Authors:S. Flores-Martínez, J. Martínez, M. Machorro-Lazo, A. García-Zapién, L. Salgado-Goytia, E. Cruz-Quevedo, M. Morán-Moguel, and J. Sánchez-Corona
The analysis of polymorphic markers within or closely linked to the cystic fibrosis transmembrane regulator (CFTR) gene is useful as a molecular tool for carrier detection of known and unknown mutations. To establish the association between mutations in the CFTR gene in western Mexican cystic fibrosis (CF) patients, the distribution of XV2c/KM19 haplotypes was analyzed by PCR and restriction enzyme digestion in 384 chromosomes from 74 CF patients, their unaffected parents, and normal subjects.The haplotype analysis revealed that haplotype B was present in 71.9% of CF chromosomes compared to 0% of non-CF chromosomes. The F508del and G542X mutations were strongly associated with haplotype B (96.7% and 100% of chromosomes, respectively). The haplotype distribution of the CF chromosomes carrying other CFTR mutations had a more heterogeneous background.Our results show that haplotype B is associated with CFTR mutations. Therefore, haplotype analysis is a suitable alternate strategy for screening CF patients with a heterogeneous clinical picture from populations with a high molecular heterogeneity where carrier detection programs are not available. In addition, it may be a helpful diagnostic tool for genetic counseling and carrier detection in the relatives of CF patients and in couples who are planning to have children.