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. A new solution for an old problem: the regiochemical distribution of the acyl chains in galactolipids can be established by electrospray ionization tandem mass spectrometry Rapid Commun . Mass Spectrom. 17 : 1982 – 1994

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C HEN , G. , C AO , P. & LIU , R. ( 2011 ): A multi-residue method for fast determination of pesticides in tea by ultraperformance liquid chromatography-electrospray tandem mass spectrometry combined with modified QuEChERS sample preparation

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. , Hoffmann , L. , and Bohn , T. ( 2010 ). Development of a multi-class method for the quantification of veterinary drug residues in feedingstuffs by liquid chromatography-tandem mass spectrometry . Journal of Chromatography A , 1217 : 6394 – 6404 . 10

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. L iu , J. , Z hao , G. , Y uan , Y. , C hen , F. & H u , X. ( 2008 ): Quantitative analysis of acrylamide in tea by liquid chromatography coupled with electrospray ionization tandem mass spectrometry . Food Chem. , 108 , 760 – 767

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Seed germination is a new beginning for the crop life cycle, which is closely related to seed sprouting and subsequent plant growth and development, and ultimately affects grain yield and quality. Salt stress is one of the most important abiotic stress factors that restrict crop production. Therefore, it is highly important to improve crop salt tolerance and sufficient utilization of saline-alkali land. In this study, we identified the phosphorylated proteins involved in salt stress response by combining SEM, 2-DE, Pro-Q Diamond staining and tandem mass spectrometry. The results showed that salt stress significantly inhibited seed germination and starch degradation. In total, 14 phosphorylated protein spots (11 unique proteins) in the embryo and 6 phosphorylated protein spots (4 unique proteins) in the endosperm were identified, which mainly involved in stress/defense, protein metabolism and energy metabolism. The phosphorylation of some proteins such as cold regulated proteins, 27K protein, EF-1β and superoxide dismutase could play important roles in salt stress tolerance.

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A comparative proteomic analysis of grain proteins during five grain developmental stages of wheat cultivar Chinese Spring (CS) and its 1Sl/1B substitution line CS-1Sl(1B) was carried out in the current study. A total of 78 differentially expressed protein (DEP) spots with at least 2-fold expression difference were detected by two-dimensional electrophoresis (2-DE). Among these, 73 protein spots representing 55 differentially expressed proteins (DEPs) were successfully identified by matrix-assisted laser desorption/ionization time-offlight tandem mass spectrometry (MALDI-TOF/TOF-MS). Differential protein spots between the two genotypes were analyzed by cluster software, which revealed significant proteome differences. There were 39 common spots (including 33 DEPs) that showed significant difference between the two lines across five grain developmental stages, of which 14 DEP spots (including 11 DEPs) were mainly involved in carbohydrate metabolism that were encoded by the genes on 1B chromosome while 25 DEP spots (including 12 DEPs) were mainly related to stress response and gluten quality that were encoded by 1S1 chromosome. These results indicated that the Sl genome harbors more stress and quality related genes that are potential valuable for improving wheat stress resistance and product quality.

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Berendsen, B.J.A., Essers, M.L., Stolker, A.A.M. & Nielen, M.W.F. (2011): Quantitative trace analysis of eight chloramphenicol isomers in urine by chiral liquid chromatography coupled to tandem mass spectrometry. J. Chromatogr. A ., 1218 , 7331

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The aim of this study was to assess the impact of hanging position of hunted pheasant carcasses (secured by the head as compared to hanging position secured by the legs) on the biogenic amine concentration in the thigh and breast muscles. The carcasses of feathered game (Phasianus colchicus), left entirely untreated after hunting and placed in a storage space at a pre-set temperature of 7 °C for 21 days were used in the study. Samples of breast and thigh muscles were taken at regular weekly intervals. Measurement of biogenic amines (putrescine, cadaverine, tyramine, tryptamine, histamine, phenylethylamine) was based on high-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry. Higher biogenic amine concentrations were detected in the muscles (both breast and thigh) of pheasants hanging by their legs compared to pheasants hanging by their heads (no statistically significant difference in biogenic amine concentration between monitored groups was, however, established). Higher concentrations of biogenic amines were found in the thigh muscles compared to breast muscles in both monitored groups. The obtained results show, that hanging the carcasses of pheasants during storage by the head is more suitable method in term of biogenic amine concentration than storing carcasses hanging by the legs.

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(AKH) precursor processing in Locusta migratoria obtained by capillary liquid chromatography-tandem mass spectrometry. Peptides 23 , 635–644. Schoofs L. New insights in

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Acta Biologica Hungarica
Authors: Parisa Rahimi Tamandegani, Doustmorad Zafari, Tamás Marik, András Szekeres, Csaba Vágvölgyi, and László Kredics

References 1. Biemann , K. ( 1990 ) Sequencing of peptides by tandem mass spectrometry and high-energy collisioninduced dissociation . Methods

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