Authors:M. Pillai, J. Gupte, T. Jyotsna, and R. Mani
Radioiodinated insulin and thyroid stimulating hormones were prepared using 1,3,4,6-tetrachloro-3, 6-diphenylglycouril (Iodogen). Conditions of iodination like concentration of iodogen, reaction time, etc., were optimized to get maximum yield. Stability studies of iodogen coated tubes were carried out over a period of time. The dependence of iodination yield on varying amounts of activity and protein concentration were investigated. Iodination yield over a range of pH was also studied. The radiolabelled hormones prepared by this method were used in radioimmunoassay and were compared with tracers prepared by the Chloramine-T method.
The reduction of99mTc by SnCl2 in the presence of 1,7-bis (2-pyridyl)-2,6-diazaheptane (DPTN) has been investigated in borate buffer solutions with pHs ranging from 6.7 to 9.5. The resulting cationic, neutral and anionic complexes, in addition to TcO2 and TcO
impurities, were separated by high voltage paper electrophoresis. The cationic complex turned into neutral and then into an anionic form via hydrolysis. These three species were also separated by gel filtration on a Bio-Gel P-2 column; their formation yields, stability, and the rate of their hydrolysis are presented as a function of the pH, and the results are discussed.
Authors:Róbert Víg, Péter Tamás Nagy, Attila Dobos, and János Nagy
high plant populations and its implications for crop yieldstability. Field Crops Research. 88. (2–3) 103 – 114.
Zhang, Z-H. et al., 2004. Genetic dissection of the relationships of biomass production and