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A bioluminescent derivative of Bacillus subtilis containing a plasmid encoding a luxAB fusion under control of a vegetative promoter and gives bioluminescence upon addition of an exogenous long-chain aldehyde has been used as test organism. Its spore populations have been produced and their heat- and radiation survival curves established. Heat-sensitization effect of pre-irradiation of spores was proven not only by colony counting but also with differential scanning calorimetry. Under a linearly programmed temperature increase, the heat destruction of spores surviving 2.5 kGy gamma irradiation resulted in at a few centigrade lower temperature than that of untreated spores. Heat denaturation endotherms in the DSC-thermogram of irradiated spores were shifted to lower temperatures as well. Comparative turbidimetric, luminometric and phase-contrast microscopic studies of untreated, heat-treated and irradiated spore populations showed that the kinetics of germination and the light emission during germination of radiation-inactivated spores were the same as those of untreated spores, revealing that the pre-formed luciferase enzyme packaged into the spores during sporulation remained intact after an irradiation dose causing 90% decrease in number of colony forming spores. Therefore, in contrast to heat-treated spores, the initial bioluminescence reading upon germination of irradiated spores does not reflect the viable count of their population.
analyzed using thermogravimetry (TG) and differential scanning calorimetry (DSC). Based on these experiments, a thermal analysis procedure to estimate SOM loss using (Δ H comb SOM ) / (Δ H β–α Qz) was developed and tested on samples from two pile burn
.: Differential scanning calorimetry study of glycerinated rabbit psoas muscle fibres in intermediate state of ATP hydrolysis. BMC. Struct. Biol., 2007, 7 , 41–50. Könczöl F. Differential
2005 Differential scanning calorimetry in life science: thermodynamics, stability, molecular recognition and application in drug design Curr Med Chem 12 2011 20
5976 Than, P., Domán, I., Lőrinczy, D.: Differential scanning calorimetry in the research of degenerative musculoskeletal disorders. Thermochimica Acta, 2004, 415 , 83
analysis The differential scanning calorimetry (DSC) thermograms of metoprolol tartrate, EC, PEG-6000 (PEG), and the physical mixture of the above three components are shown in Figs 3–6 , respectively. Metoprolol had shown the melting endotherm
rotator cuff in a rabbit model using calorimetry. [A rotátorköpeny zsíros infiltrációjának vizsgálata kalorimetriával nyúl modellállaton.]. Magyar Traumatológia, Ortopédia, Kézsebészet és Plasztikai Sebészet, 2007, 50 , 144–153. [Hungarian
: differential scanning calorimetry; NMR: nuclear magnetic resonance; UV: ultraviolet. Materials and Methods Materials M. hostilis Barks and inner barks from M. hostilis
°C in a glass container [ 13 , 14 ]. Characterization The physicochemical compatibility between formulation with final excipient used in the study for drug-excipient interaction by differential scanning calorimetry
Differential scanning calorimetry (DSC) The DSC study was performed to find incompatibility and interaction between ISR, lipids, polymer, and formulations mixture via rutin. The thermal analysis (2.5 g) was performed on aluminum pan (used empty