Search Results

You are looking at 1 - 10 of 13 items for :

  • "Blood serum" x
  • User-accessible content x
Clear All

Abstract  

Differential scanning microcalorimetry (DSC) and UV–VIS absorption spectroscopy were used to obtain the characteristics of blood serum from newborn rat’ after maternal treatment with cyclophosphamide in comparison with control. The obtained DSC curves reveal a complex endothermic peak due to the unfolding process of various serum proteins. Thermal profiles and absorption spectra of blood serum are sensitive to the age of newborns as well as to effect of maternal administration of cyclophosphamide. The most significant disturbances in serum proteome were observed for 14-day old newborns. The thermodynamic parameters: enthalpy change (∆H), the normalized first moment (M1) of the thermal transition with respect to the temperature axis and the ratio of C p ex at 70 and 60 °C describing denaturation contributions of globulin forms in respect to unliganded albumin with haptoglobin was estimated. Moreover, the second derivative spectroscopy in the UV region was used to resolve the complex protein spectrum. The differences in blood serum detected by DSC and UV–VIS confirm a potential usefulness of these methods for diagnostic and monitoring changes with age as well as the pathological state of blood serum.

Open access

(corvitin) in a dose 100 mg/kg of animal weight intramuscularly for 7 days (from the 7 th to the 14 th day). On the 14 th day, the experimental rats were sacrificed by bleeding under thiopental anesthesia. For further testing, blood serum was selected

Open access

day). On the 14 th day, experimental animals were exsanguinated under thiopental anesthesia. For further research, the blood serum was selected. Superoxide dismutase (SOD), catalase activity, and ceruloplasmin maintenance were determined in the serum

Open access

proteins of the basic character (OMP 430 ) in blood serum (by 1.75 times, p  < 0.01), but later, on 14th day, this index changed in the opposite direction, that is, it began to decrease (by 1.24 times, p  < 0.01) as compared with the animals on the 7th

Open access

. Peeters , M. , Sulon , J. , Beckers , J. F. , Ledoux , D. and Vandenheede , M. ( 2011 ): Comparison between blood serum and salivary cortisol concentrations in horses using an adrenocorticotropic hormone challenge . Equine Vet. J. 43 , 487

Open access

). *Statistically significant difference compared with non-Su groups The results of biochemical analysis of the blood serum are listed in Table  II . Neither the RT nor the administration of Su modified significantly the mean

Open access

the contents of total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) in mouse blood serum, decrease the contents of monoamine oxidase (MAO) and malonaldehyde (MDA) in mouse blood serum and liver, and decrease levels of lipofuscin (LF) in

Open access

); There is no significant difference between the mean results of the measurements (T1–T4) The lactate values taken from the blood serum during the T1, T2, T3, T4 measurements are shown in Table 3 . Table 3. Serum lactate values of the subjects before and

Open access

were developed for the determination and quantification of propranolol in various biological fluids such as blood, serum, plasma, and urine. Consequently, many HPLC–ultraviolet (UV) methods as well as HPLC–fluorescence or mass spectroscopic methods were

Open access

medical applications [ 1 ]. These polymers are usually supplied as either sodium or calcium form. In medicine, it is used as a potassium binder for treating acute and chronic kidney disease in people with hyperkalaemia (abnormal high blood serum potassium

Open access