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Abstract
Differential scanning microcalorimetry (DSC) and UV–VIS absorption spectroscopy were used to obtain the characteristics of blood serum from newborn rat’ after maternal treatment with cyclophosphamide in comparison with control. The obtained DSC curves reveal a complex endothermic peak due to the unfolding process of various serum proteins. Thermal profiles and absorption spectra of blood serum are sensitive to the age of newborns as well as to effect of maternal administration of cyclophosphamide. The most significant disturbances in serum proteome were observed for 14-day old newborns. The thermodynamic parameters: enthalpy change (∆H), the normalized first moment (M1) of the thermal transition with respect to the temperature axis and the ratio of C p ex at 70 and 60 °C describing denaturation contributions of globulin forms in respect to unliganded albumin with haptoglobin was estimated. Moreover, the second derivative spectroscopy in the UV region was used to resolve the complex protein spectrum. The differences in blood serum detected by DSC and UV–VIS confirm a potential usefulness of these methods for diagnostic and monitoring changes with age as well as the pathological state of blood serum.
well-known therapeutic drug applied in treatment of alcoholism to prevent drinking alcohol by producing unpleasant side effects, such as headaches, disturbances of the circulatory system performance, disturbances of the alimentary tract performance
column was used. We observed that the separation of these two peaks is difficult because of the disturbance by GC–nitrogen phosphorus detection proposed by other authors and when the cation-exchange resin was not used [ 13 - 16 ]. Figure
Instrumentation and calibration of the Calvet calorimeter
Enthalpy of solution of PrBr3 at standard conditions
overcooling the compound and kinetic disturbances of process at low temperature. The enthalpy of solution for the Rb 3 PrBr 6 at standard conditions has not been obtained, but the results of experiment leads to unexpected conclusion that this compound does
anesthetics in the form of a free base or in the ionized state can induce analgesia either by general physicochemical disturbance of the neuron myelin sheath or by closing ionic sodium channels in the neuronal membrane [ 2 ]. Fig. 1
disturbance, central system inhibition, symptoms such as loss of consciousness, respiratory inhibition and so on [ 2–4 ]. HCP may be found in cosmetics due to the production conditions of cosmetic raw materials, the production process, and improper storage of
[ 2 ]. PCDD/Fs bind to cell aryl hydrocarbon receptors (AhRs) causing toxic effects [ 3 ]. This binding changes the expression of genes that are mediated by AhR and may affect cell growth and differentiation, result in hormonal disturbances
: osteopenia which has been observed during the Project Gemini, sleep disturbances [ 1 ], or space motion sickness, just to mention a few [ 2 ]. In addition, the short shelf life (typically less than 1 year) of pharmaceuticals in space adds another layer of
O than forest and arable land, possibly due to high SOM content and little soil disturbance, resulting in high nutrient adsorption and low leaching rate. R ODRIGUES et al. (2017) recorded that potassium content in minimum tillage was significantly
disturbances and easier method transferability. The proposed method shows better HPLC performance with advances over the reported methods investigating THZ and FLM. These advances include chromatographic characters and general separation method improvements
