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A sensitive and reproducible high-performance liquid chromatographic (HPLC) method for determination of talinolol (TAL) in rat plasma was developed and validated using pindolol (PIN) as an internal standard. Both TAL and PIN were separated on a Zorbax Eclipse XDB C18 column by gradient elution with 0.1% aqueous formic acid and acetonitrile as the mobile phase. Detection was performed using fluorescence measurement at λ ex 249 nm and λ em 333 nm. The method was validated and found to be linear in the range of 10–2000 ng mL−1. The limit of quantification was 10 ng mL−1 based on 100 μL of plasma. The variations for intra- and inter-day precision were less than 10%, and the accuracy values were between 92% and 102.9%. The extraction recoveries were more than 82%. The assay was successfully applied to an in-vivo pharmacokinetic study of TAL in rats that were administered a single oral dose of 10 mg kg−1 TAL. The maximum concentration (C max) and the area under the plasma concentration-time curve (AUC0–12) were 0.369 ± 0.024 μg mL−1 and 1.429 ± 0.027 μg h mL−1, respectively. The modulatory effect of apricot juice on P-glycoprotein-related efflux carriers was also investigated. Co-administration of apricot juice resulted in a significant increase of the amount of TAL in plasma (C max and AUC0–12 were 0.679 ± 0.021 and 2.357 ± 0.079, respectively; p < 0.05).

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Progress in Agricultural Engineering Sciences
Authors: Viktória Zsom-Muha, Lien Le Phuong Nguyen, László Baranyai, Géza Hitka, Zsuzsanna Horváth-Mezőfi, Gergő Szabó, and Tamás Zsom

selected per side. These six individual points were used for DA-meter® measurements and for chlorophyll fluorescence measurements by the Walz monitoring-PAM fluorometer in order to characterize the change in surface color related chlorophyll content during

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assay reagents on a glass chip followed by fluorescence measurements across the gradient. They validated the assay using known BACE1 inhibitors, determining IC 50 values using the flow bioassay and traditional well plate-based assays, demonstrating very

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European Journal of Microbiology and Immunology
Authors: Carlos Florindo, Cinthia Alves Barroco, Inês Silvestre, Vera Damião, João Paulo Gomes, Barbara Spellerberg, Ilda Santos-Sanches, and Maria José Borrego

-iT PicoGreen was added to an equal volume of each supernatant, previously diluted in 1× Tris–EDTA (TE) buffer. After 5 min of incubation at room temperature, in the dark, we proceeded to the fluorescence measurement (Fluorimeter – Anthos Zenith 3100) in 96-well

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