Authors:Zofia Drzazga, Katarzyna Michalik, Tomasz Halat, Anna Michnik, and Henryk Trzeciak
Differential scanning microcalorimetry (DSC) and UV–VIS absorption spectroscopy were used to obtain the characteristics of
blood serum from newborn rat’ after maternal treatment with cyclophosphamide in comparison with control. The obtained DSC
curves reveal a complex endothermic peak due to the unfolding process of various serum proteins. Thermal profiles and absorption
spectra of blood serum are sensitive to the age of newborns as well as to effect of maternal administration of cyclophosphamide.
The most significant disturbances in serum proteome were observed for 14-day old newborns. The thermodynamic parameters: enthalpy
change (∆H), the normalized first moment (M1) of the thermal transition with respect to the temperature axis and the ratio of Cpex at 70 and 60 °C describing denaturation contributions of globulin forms in respect to unliganded albumin with haptoglobin
was estimated. Moreover, the second derivative spectroscopy in the UV region was used to resolve the complex protein spectrum.
The differences in blood serum detected by DSC and UV–VIS confirm a potential usefulness of these methods for diagnostic and
monitoring changes with age as well as the pathological state of blood serum.
Authors:B. Mammel, T. Kvárik, Zs. Szabó, J. Gyarmati, T. Ertl, J. Farkas, Zs. Helyes, T. Atlasz, D. Reglődi, and P. Kiss
smoking. The aim of the present study was to investigate the influence of maternal smoking during pregnancy on the early physical and neurobehavioral development of newbornrats. Our hypothesis was that maternal smoking during pregnancy interferes with
., Gyenge, M., Hantos, M., et al.: Transgenerational
hormonal imprinting caused by vitamin A and vitamin D treatment of newbornrats.
Alterations in the biogenic amine contents of the adult brain. Brain Dev., 2009
Tekes, K., Gyenge, M., Hantos, M., et al.: Transgenerational hormonal imprinting caused by vitamin A and vitamin D treatment of newbornrats. Alterations in the biogenic amine contents of the adult brain. Brain Dev., 2009, 31 , 666
, Jacques Miller removed the thymus from newbornrats, which caused a dramatic effect on the condition and immunity of the animals  , as a part of the lymphocyte population (later named T cells) disappeared and a fatal wasting disease developed. Further