of TCM chromatographic fingerprinting is playing an increasingly important role in the quality control of TCM, and has proven to be practical and scientific [ 9 ].
Recently, the chromatographic fingerprint technique has been introduced as a
fingerprinting is playing an increasingly important role in the quality control of TCM, and has proven to be practical and scientific [ 9 ]. Recently, the chromatographic fingerprint technique has been introduced as a tool to evaluate the quality of herbal
Authors:Yanqin Zhu, Ping Du, Shaojun Huang, Qinhong Yin, and Yaling Yang
cultivars of M. oleifera using HPLC with photodiode array detection and electrospray ionization–MS (HPLC–PDA–ESI–MS) analysis [ 20 ].
Fingerprinting techniques can provide an overall profile of almost every component of medicinal plants and are
Authors:Tong Chen, Xingpu Qi, Mingjie Chen, Daoli Lu, and Bin Chen
chemometric tools to identify the geographical origin of yellow wine based on the fingerprint of volatile organic compounds. In this article, an approach was developed based on a color mixing method to provide visual color differences between different 2D
Authors:Min Zhang, Zhenling Zhu, Mengyue Wang, Jincai Hou, Yinqing Li, and Xiaobo Li
strategy was limited because Q-TOF and Qtrap-MS have not been widely equipped in many pharmaceutical factories in China. In the present study, the commonly equipped diode array detector (DAD) was initially applied for the quantitative fingerprint analysis
Authors:Long Wang, Yuan-Yuan Jiang, Li Zhang, Tao Wang, Rui-Wu Yang, Chun-Bang Ding, Xiao-Li Wang, and Yong-Hong Zhou
A high-performance liquid chromatography (HPLC) method has been developed for the simultaneous identification and quantification of active compounds (cryptotanshinone, dihydrotanshinone I, tanshinone IIA, tanshinone I, salvianolic acid A, salvianolic acid B, protocatechuic aldehyde, and rosmarinic acid) contained in traditional Chinese folk medicine Salvia przewalskii Maxim. The herb samples (including wild, cultivated, and yin pian) from fourteen main regions were investigated. Chromatographic separation was performed on an Agilent Eclipse XDB-C18 reserved-phase column (250 mm × 4.6 mm i.d., 5 μm) using gradient elution with water-formic acid (99.9: 0.1, v/v) and acetonitrile at a flow rate of 0.8 mL min−1, an operating temperature of 30 °C, and a wavelength of 275 nm. Similarity analysis (SA), principal component analysis (PCA), and hierarchical cluster analysis (HCA) were used to analyze the data based on fingerprints. For fingerprint analysis, 27 peaks were selected as the common peaks to evaluate the similarities among different samples. The results of SA showed that the method permits to obtain desired linearity, precision, accuracy, and recovery. All samples were divided into three categories by PCA and HCA, and the concentration of the eight bioactive compounds varied significantly from different regions. It was demonstrated that chromatographic fingerprinting by HPLC combined with the simultaneous determination of eight bioactive compounds was a helpful method for the quality control of S. przewalskii.
Authors:Tong Chen, Chunyou Liu, Bin Chen, and Yongchun Huang
significance and broad application prospect. At present, many researchers have studied the flavor composition of rapeseed oil. Based on the flavor fingerprint, Gas chromatograph-Mass Spectrometry (GC-MS) technology has been proved suitable for vegetable oil
Authors:W.-Y. Liu, M.-L. Wei, C.-Y. Wu, H.-Y. Zhu, F. Feng, and N. Xie
Patrinia scabra Bunge has long been used in clinic as a traditional Chinese medicine for treating leukemia and cancer and regulating host immune response. Despite their wide use in China, no report on system analysis on their chemical constituents is available so far. The current study was designed to profile the fingerprint of ethyl acetate extract of it, and in addition, to characterize the major fingerprint peaks and determine their quantity. Therefore, a detailed gradient high-performance liquid chromatography was described to separate more than 30 compounds with satisfactory resolution in P. scabra Bunge. Based on the chromatograms of 10 batches samples, a typical high-performance liquid chromatographic (HPLC) fingerprint was established with 23 chromatographic peaks being assigned as common fingerprint peaks. Furthermore, a quadrupole time of flight mass spectrometry (Q-TOF/MS) was coupled for the characterization of major compound. As (+)-nortrachelogenin was the most predominant compound in P. scabra Bunge, the quantification on it was also carried out with the method being validated. As a result, (+)-nortrachelogenin was found to be from 1.33 to 2.21 mg g−1 in this plant material. This rapid and effective analytical method could be employed for quality assessment of P. scabra Bunge, as well as pharmaceutical products containing this herbal material.
Authors:M.A. Hawrył, M. Niemiec, K. Słomka, M. Waksmundzka-Hajnos, and G. Szymczak
Micro-thin-layer chromatography in two dimensional (2D-mTLC) mode in normal and reversed phase systems by use of diol bonded stationary phase was applied to make fingerprints of 11 species of Mentha genus and two finished pharmaceutical products.
Nonaqueous eluents (propan-2-ol or ethyl acetate dissolved in n-heptane) were used in normal phase systems. Mixtures of acetonitrile with water were used in reversed phase chromatographic systems.
Optimization of one dimensional systems was performed by determining of RF vs. composition of mobile phases dependencies for standards occurring in various species of Mentha. Most selective eluents were chosen to optimize two-dimensional systems by creating RF in normal-phase (NP) systems vs. RF in reversed-phase (RP) systems correlations.
2D-mTLC on diol polar bonded stationary phase were optimized to separate phenolic compounds and make fingerprints of examined plant materials and this method was never applied earlier in the chromatographic analysis.
Authors:J. Wang, M. Liu, X. Tong, W. Peng, H. Cao, and W. Su
Shenqi Fuzheng Injection (SFI) is a traditional Chinese medicine injection, widely used to enhance immune function of clinical cancer patients undergoing chemotherapy. In this study, a high-performance liquid chromatography-diode array detection-evaporative light scattering detection (HPLC-DAD-ELSD) method was established for quality control of SFI, which could simultaneously semiquantitatively reflect the constituents displayed in the chromatographic profile of SFI. The relative retention time and relative peak areas of the 21 common peaks related to the reference peak were calculated. The validity and advantage of this method were validated by systematically comparing chromatograms of 10 batches of SFI samples with the analytical methods of principal component analysis and angle cosine method recommended by the State Food and Drug Administration of China. Moreover, a total of 21 constituents of SFI were identified or tentatively characterized in the fingerprint via ultrafast liquid chromatography-diode array detection-quadrupole time-of-flight (UFLC-DAD-Q-TOF) tandem mass spectrometry technique on the basis of the retention time, ultraviolet spectra, fragmentation patterns, and reported literatures. All the results proved that the technique was useful in comprehensive quality evaluation of SFI and further study.