Search Results

You are looking at 1 - 6 of 6 items for :

  • "melting curves" x
  • User-accessible content x
Clear All
Orvosi Hetilap
Authors: Orsolya Dóra Ács, Bálint Péterfia, Péter Hollósi, Irén Haltrich, Ágnes Sallai, Andrea Luczay, Karin Buiting, Bernhard Horsthemke, Dóra Török, András Szabó, and György Fekete

–12. 12 Wang W, Law HY, Chong SS. Detection and discrimination between deletional and non-deletional Prader–Willi and Angelman syndromes by methylation-specific PCR and quantitative melting curve analysis

Open access
European Journal of Microbiology and Immunology
Authors: Ali Konaté, René Dembélé, Assèta Kagambèga, Issiaka Soulama, Wendpoulomdé A. D. Kaboré, Emmanuel Sampo, Haoua Cissé, Antoine Sanou, Samuel Serme, Soumanaba Zongo, Cheikna Zongo, Alio Mahamadou Fody, Nathalie K. Guessennd, Alfred S. Traoré, Amy Gassama-Sow, and Nicolas Barro

Escherichia coli by use of melting-curve analysis and real-time multiplex PCR . J Clin Microbiol 46 , 1752 – 1757 ( 2008 ) 24. Okeke IN

Open access

point is ambiguous, since for pure water the crystallization point was found to be −22 °C. In turn, in the process of butter heating (the melting curve, Fig. 1 ), endothermic transitions connected with the solid–liquid phase transition take place. At a

Open access

confirm the specificity of the amplification reactions, a melting curve was recorded. Each sample was replicated three times; the value of the threshold cycle (Ct) was the same as that of the corresponding mean. The relative fold expression of each mRNA

Open access
European Journal of Microbiology and Immunology
Authors: Hans Kollenda, Ralf Matthias Hagen, Miriam Hanke, Sandra Rojak, Rebecca Hinz, Lars Wassill, Sven Poppert, Egbert Tannich, and Hagen Frickmann

with DNA filter paper, loop-mediated isothermal amplification, and melting curve analysis . Jpn J Infect Dis . 2009 ; 62 : 20 – 5 . 40. Singh R , Savargaonkar D , Bhatt R , Valecha N . Rapid detection of Plasmodium vivax in saliva and blood

Open access
Journal of Behavioral Addictions
Authors: Mitra-Sadat Sadat-Shirazi, Nasim Vousooghi, Bentolhoda Alizadeh, Seyed Mohammad Makki, Seyed Zeinolabedin Zarei, Shahrzad Nazari, and Mohammad Reza Zarrindast

Biosystems, USA). For each gene, the real-time PCR reaction was adjusted in such a way that a single product was amplified for each primer pair. The optimized annealing temperature of reactions was from 60 to 63 °C. Observing a single peak in melting curve

Open access