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Biochemical and molecular screening of varieties of chili plants that are resistant against Fusarium wilt infection

European Journal of Microbiology and Immunology
Volume/Issue:
Volume 8: Issue 1
DOI:
https://doi.org/10.1556/1886.2017.00031
Authors: Sobiya Shafique, Shazia Shafique, and Aqeel Ahmad

gene due to its strong recommendation by Coker and Davies [ 22 ]. A primer set used for studying α-Tubulin expression was the same as used by Xu and Shi [ 23 ], at their recommended thermocyclic conditions in GeneAmp-2700 thermocycler (Applied

Open access

Humanization of the blood–brain barrier transporter ABCB1 in mice disrupts genomic locus — lessons from three unsuccessful approaches

European Journal of Microbiology and Immunology
Volume/Issue:
Volume 8: Issue 3
DOI:
https://doi.org/10.1556/1886.2018.00008
Authors: Markus Krohn, Thomas Wanek, Marie-Claude Menet, Andreas Noack, Xavier Declèves, Oliver Langer, Wolfgang Löscher, and Jens Pahnke

photometrically. cDNA was synthesized using the High Capacity RNA-to-cDNA Kit (Applied Biosystems, USA). Gene expression was analyzed with qRT-PCR using TaqMan Hybridization Probes. Primer sets with according TaqMan probes for Abcb1a (Mm00440761_m1), human ABCB

Open access