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Bacterial strains with inhibitory effect on Salmonella Hartford, Listeria monocytogenes, Yersinia enterocolitica, and Escherichia coli, respectively, were isolated. Out of the 64 bacteria originated from food processing environments, 20 could inhibit at least one of the tested pathogens, and it was proved that growth decline of the pathogenic bacteria was more remarkable by co-culturing than by using cell-free supernatants of the isolates. Seven different genera (Pseudomonas, Bacillus, Paenibacillus, Macrococcus, Staphylococcus, Serratia, and Rothia) reduced the pathogens’ growth during the time period of analysis, and the strongest inhibitory effect was observed after 24 h between 15 and 30 °C. Sensitivity of the tested human pathogenic bacteria against the inhibitory strains was distinct, as Y. enterocolitica could be inhibited by numerous isolates, while S. Hartford proved to be the most resistant. Our results reveal that the isolated bacteria or their excreted metabolites could hinder pathogen growth when used in sufficient quantities.

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Abstract

Previously isolated Pseudomonas lundensis CP-P-5 had antagonistic activity against Salmonella Hartford, Yersinia enterocolitica, and Escherichia coli. In this study, determination of its antagonistic mechanism and potential field of application in food industry was aimed. Using cellophane-test and microcultures of the test strain's cell-free supernatant mixed with the pathogens, our results showed that cells of P. lundensis CP-P-5 and its concentrated cell-free supernatants were effective against the foodborne bacteria, and the supernatants contained more than one compound responsible for inhibitory activity. Searching for the antagonistic compound, NaOH, protease, and heat treatments were done to the supernatants, and proteolytic activity and siderophore production were also tested using the antagonistic strain. Our results support the potential applicability of P. lundensis CP-P-5 as a bioprotective agent against foodborne pathogens in food processing environments.

Open access