Search Results

You are looking at 1 - 6 of 6 items for

  • Author or Editor: K. Kiss x
  • Refine by Access: Content accessible to me x
Clear All Modify Search

Purpose

This study aimed to examine the effectiveness of CO2 laser-assisted sclerectomy surgery (CLASS) in eyes with primary open-angle glaucoma (POAG) showing progression in spite of maximal local antiglaucomatous therapy.

Materials and methods

Patients with progressive POAG received CLASS treatment. We performed CLASS on 15 eyes (eight males and seven females). The primary endpoint was the change in the intraocular pressure (IOP), and additionally best spectacle-corrected visual acuity (BSCVA), C/D ratio (cup-to-disc), as well as use of antiglaucomatous drops were also investigated. Following the preoperative assessment, measurements were performed at 6-month follow-up.

Results

The average preoperative IOP was 26.13 ± 6.79 mmHg that dropped to 9.57 ± 4.09 mmHg at 1 day. IOP was stable at 1 month, 3 months, and 6 months. The BSCVA decreased to the 1-day and 1-week follow-up but returned to its original value to the 1-month check-up. Preoperatively, all patients were on maximal antiglaucoma drop therapy, after CLASS none of the patients needed antiglaucomatous treatment at 1 month. However, at 3 months, one of them needed antiglaucoma drops. C/D ratio showed non-significant changes.

Conclusions

CLASS procedure was found to be effective in decreasing IOP in POAG patients whose IOP was not compensated with maximal antiglaucomatous local therapy; patients needed significantly less local therapy following the CO2 laser surgery.

Open access
Acta Alimentaria
Authors:
K. Berisha
,
H. Bytyçi
,
Zs. Mednyánszky
,
E. Kiss
, and
L. Simon-Sarkadi

Abstract

To our knowledge, there is a lack of information on the nutrient composition of Busha cattle milk with special regard to its amino acid and biogenic amine contents. The Busha cattle breed is known to be highly resistant to various diseases and well-adapted to the extensive breeding conditions of the Balkan Peninsula. Busha cow milk contains an average of 13.47% dry matter, 4.34% fat, 3.72 % protein, and 4.32% lactose. Significant differences were detected (P < 0.05) in the amino acid compositions of the milk of different Busha cattle strains of Kosovo. Glutamic acid, proline, leucine, aspartic acid, lysine, and valine represented 68% of the total amino acid content. Essential amino acids, branched-chain and sulphur-containing amino acids were found in substantial amounts in the milk samples. Among the biogenic amines, however, spermine (0.16 mg kg−1) and cadaverine (0.09 mg kg−1) were present in low concentrations. Due to these excellent qualities of the Busha cow milk, preservation of this cattle breed is of great importance. Developing sustainable and secured breeding and feeding programs for this endangered cattle breed of the Balkan Peninsula should also be a high priority.

Open access

Summary

This article is the first report describing a new validated method to determine the content of HupA in Huperzia selago (Huperziaceae) from wild population and obtained in in vitro culture using the chaotropic mobile phase. An aqueous-organic (acetonitrile) mobile phase with an added chaotropic salt (NaPF6) was used. The system of mobile phases ensured very high selectivity and efficiency at up to N = 6683 ± 963 theoretical plates calculated for isocratic mode. A Hypercosil GOLD column, C18 250 × 4.6 mm, and a Hypercosil GOLD precolumn, 5UM 10 × 4 mm, were employed for detection at four wavelengths, 230 nm being analytical. The regression coefficient (R 2) of the calibration was 0.9993 over the range 25–1252 μg mL−1. The recovery rates were 98.36–105.1% with RSD <2.9%. The intra- and inter-day precisions, expressed as RSD, ranged from 1.2% to 2.7%. LOD for HupA was 14 ng mL−1 for a signal-to-noise ratio of 3:1. The limit of quantification was 140 ng mL−1. The huperzine A (HupA) content of the plant material ranged from 0.65 mg g−1 dry weight (d.w.) to 1.59 mg g−1 d.w. (material from wild plants) and from 0.44 to 1.10 mg g−1 d.w. (material from in vitro cultures). Interestingly, in our study, plants of H. selago derived from wild population had one of the highest HupA concentrations recorded for a club moss (1.59 mg g−1 d.w.). The findings demonstrate that H. selago, found in Europe and North America, is an alternative source of HupA, richer than H. serrata. In order to confirm that HupA was present in the alkaloid extracts, HPLC-ESI-MS/MS analyses of the patterns were performed in the positive ion mode. The fragmentation quasi-molecular ion of the standard HupA (m/z = 243, [M+H]+) and the ion with m/z = 243 found in the samples were identical, confirming the compound as HupA.

Open access
Progress in Agricultural Engineering Sciences
Authors:
Szilvia Bánvölgyi
,
Eszter Dusza
,
Fiina K. Namukwambi
,
István Kiss
,
Éva Stefanovits-Bányai
, and
Gyula Vatai

Abstract

Similarly to other industries wineries also increasingly attempt to minimize and utilize waste to protect our environment. The aim of this study was to determine the optimal parameters (temperature, solvent concentration, and time) of extracting total polyphenol content (TPC) from Tokaji Aszú marc using two different extraction solvents: ethanol–water and isopropanol–water (1:4 solid/liquid ratio). The extractions were achieved based on Central Composite Design with Response Surface Method (CCRD–RSM). The optimal extraction parameters in the case of ethanol–water solvent: 60 °C temperature, 59.5% ethanol concentration in solvent, 5 h. At these parameters the probable TPC concentration is 23966.2 uM GAE/L. The optimal extraction parameters in the case of isopropanol–water solvent: 60 °C temperature, 52% ethanol concentration in solvent, 5 h. At these parameters the probable TPC concentration is 7188.44 uM GAE/L. In both cases the binary solvent was better than the mono-solvent. Ethanol–water solvent was more efficient than the isopropanol–water solvent.

Open access
Orvosi Hetilap
Authors:
Taha El Hadj Othmane
,
István Kiss
,
János Nemcsik
,
Cs. Bertalan Fekete
,
György Deák
,
József Egresits
,
Erzsébet Fodor
,
K. Zsófia Németh
,
Tamás Szabó
,
Miklós Szathmári
, and
András Tislér

Korábbi vizsgálatok eredményei alapján az érfali tágulékonyság paraméterei összefüggést mutatnak a cardiovascularis mortalitással hemodializált betegekben. A különböző paraméterek relatív prognosztikus értékét ugyanakkor egy közös kohorszban eddig nem vizsgálták. Módszer: Dialízis előtt és után 98 betegnél mértük a carotis-femoralis pulzushullám terjedési sebességét, a carotis augmentációs indexét, a carotis pulzusnyomását és a carotis-brachialis pulzusnyomás amplifikációját. A betegeket 29 hónapig (medián) (tartomány 1–35) követtük, majd a cardiovascularis mortalitás és a kiinduláskor mért tágulékonysági paraméterek közötti összefüggést vizsgáltuk log-rank tesztek, illetve a korhoz, diabeteshez és korábban meglévő cardiovascularis megbetegedéshez illesztett Cox-féle regressziós modellek alkalmazásával. Eredmények: A követés alatt 40 beteg halt meg (mortalitási ráta 20,7/100 betegév), köztük 25-en cardiovascularis ok következtében. A dialízis előtt és után mért pulzushullám-terjedési sebességet tercilisei, illetve a dialízis előtt mért pulzusnyomás-amplifikáció tercilise szignifikáns összefüggést mutattak a cardiovascularis mortalitással (log-rank p-értékek 0,012 és 0,011 a pre- és posztdialízis pulzushullám-terjedési sebesség, illetve <0,001 és 0,321 a pre- és posztdialízis pulzusnyomás-amplifikáció esetén). Az augmentációs indexek, illetve a carotispulzusnyomás-értékek nem álltak összefüggésben a cardiovascularis mortalitással. Cox-modellben az 1 m/s-mal gyorsabb pre- és posztdialízis pulzushullám-terjedési sebességéhez tartozó relatív rizikó 1,24 (1,07–1,44) és 1,17 (1,06–1,28) volt. 10%-kal kisebb predialízispulzusnyomás-amplifikációval járó rizikónövekedés 41% (3–92%) volt. Egy közös modellben vizsgálva mind a predialízispulzushullám-terjedési sebesség, mind a pulzusnyomás-amplifikáció szignifikáns összefüggést mutatott a cardiovascularis túléléssel [relatív rizikó: 1,23 (1,07–1,42) és 1,39 (1,02–1,89)]. Következtetés: Hemodializált betegekben az érfali tágulékonyságot leíró különböző paraméterek közül a pulzushullám-terjedési sebesség a mérés idejétől független, konzekvens összefüggést mutat a cardiovascularis mortalitással. Ugyanakkor a predialízispulzusnyomás-amplifikációs érték további prognosztikus információt hordoz.

Open access
Physiology International
Authors:
Zs. Sári
,
T. Kovács
,
T. Csonka
,
M. Török
,
É. Sebő
,
J. Toth
,
D. Tóth
,
E. Mikó
,
B. Kiss
,
D. Szeőcs
,
K. Uray
,
Zs. Karányi
,
I. Kovács
,
G. Méhes
,
P. Árkosy
, and
P. Bai

Abstract

Breast cancer is characterized by oncobiosis, the abnormal composition of the microbiome in neoplastic diseases. The biosynthetic capacity of the oncobiotic flora in breast cancer is suppressed, as suggested by metagenomic studies. The microbiome synthesizes a set of cytostatic and antimetastatic metabolites that are downregulated in breast cancer, including cadaverine, a microbiome metabolite with cytostatic properties. We set out to assess how the protein expression of constitutive lysine decarboxylase (LdcC), a key enzyme for cadaverine production, changes in the feces of human breast cancer patients (n = 35). We found that the fecal expression of Escherichia coli LdcC is downregulated in lobular cases as compared to invasive carcinoma of no special type (NST) cases. Lobular breast carcinoma is characterized by low or absent expression of E-cadherin. Fecal E. coli LdcC protein expression is downregulated in E-cadherin negative breast cancer cases as compared to positive ones. Receiver operating characteristic (ROC) analysis of LdcC expression in lobular and NST cases revealed that fecal E. coli LdcC protein expression might have predictive values. These data suggest that the oncobiotic transformation of the microbiome indeed leads to the downregulation of the production of cytostatic and antimetastatic metabolites. In E-cadherin negative lobular carcinoma that has a higher potential for metastasis formation, the protein levels of enzymes producing antimetastatic metabolites are downregulated. This finding represents a new route that renders lobular cases permissive for metastasis formation. Furthermore, our findings underline the role of oncobiosis in regulating metastasis formation in breast cancer.

Open access