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Summary

A stability-indicating gradient reverse-phase liquid chromatographic method was developed for the quantitative determination of process-related impurities and forced degradation products of oxcarbazepine in pharmaceutical formulation. The method was developed by using Inertsil cyano (250 × 4.6 mm) 5 μm column with mobile phase containing a gradient mixture of solvent A (0.01 M sodium dihydrogen phosphate, pH adjusted to 2.7 with orthophosphoric acid and acetonitrile in the ratio of 80:20 v/v) and B (50:40:10 v/v/v mixture of acetonitrile, water, and methanol). The flow rate of mobile phase was 1.0 mL min−1. Column temperature was maintained at 25°C and detection wavelength at 220 nm. Developed reverse-phase high-performance liquid chromatography (RP-HPLC) method can adequately separate and quantitate five impurities of oxcarbazepine, namely imp-A, imp-B, imp-C, imp-D, and imp-E. Oxcarbazepine was subjected to the stress conditions of oxidative, acid, base, hydrolytic, thermal, and photolytic degradation. Oxcarbazepine was found to degrade significantly in acid, base, and oxidative stress conditions. The degradation products were well resolved from oxcarbazepine and its impurities. The developed method was validated as per International Conference on Harmonization (ICH) guidelines with respect to specificity, linearity, limit of detection and quantification, accuracy, precision, and robustness.

Open access

Summary

A simple, selective, and stability-indicating reverse phase liquid chromatographic method has been developed and validated for the simultaneous determination of impurities and forced degradation products of quetiapine fumarate. The chromatographic separation was achieved on Inertsil-3 C8, 150 mm × 4.6 mm, 5 μm column at 35°C with UV detection at 217 nm using gradient mobile phase at a flow rate of 1.0 mL/min. Mobile phase A contains a mixture of 0.01 M di-potassium hydrogen orthophosphate (pH 6.8) and acetonitrile in the ratio of 80:20 (v/v), respectively, and mobile phase B contains a mixture of 0.01 M di-potassium hydrogen orthophosphate (pH 6.8) and acetonitrile in the ratio of 20:80 (v/v), respectively. The drug product was subjected to the stress conditions of oxidative, hydrolysis (acid and base), hydrolytic, thermal, and photolytic degradation. Quetiapine fumarate was found to degrade significantly in acid, base, and oxidative stress conditions. The degradation products were well resolved from main peak and its impurities. The mass balance was found to be in the range of 96.6–102.2% in all the stressed conditions, thus proved the stability-indicating power of the method. The developed method was validated as per ICH guidelines with respect to specificity, linearity, limit of detection and quantification, accuracy, precision, and robustness.

Open access

Summary

Root exudates were obtained from three mangrove species, viz. Bruguiera gymnorrhiza, Excoecaria agallocha, and Heritiera fomes. Spot tests revealed the presence of, presumably, phenolic compounds in the exudates. Paper chromatography revealed two spots each for B. gymnorrhiza and H. fomes and a single spot for E. agallocha. GC-MS analysis suggested the presence of aminopyrine, palmitic acid, stearic acid, di-n-propyl ether, and 2,5-anhydrogluconic acid in B. gymnorrhiza exudates, aminopyrine and palmitic acid in E. agallocha exudates, and aminopyrine, palmitic acid, and 2,5-anhydrogluconic acid in H. fomes exudates.

Open access
Acta Chromatographica
Authors:
M. Ganesh
,
B. Thangabalan
,
R. Patil
,
D. Thakur
,
A. Kumar Kumar
,
M. Vinoba
,
S. Ganguly
, and
T. Sivakumar

Summary

A rapid, simple and validated reversed-phase high-performance liquid chromatographic method has been developed for analysis of oxaprozin in pharmaceutical dosage forms. Oxaprozin was separated on an ODS analytical column with a 45:55 (v/v) mixture of acetonitrile and triethanolamine solution (5 mm, pH 3.5 ± 0.05, adjusted by addition of 85% phosphoric acid) as mobile phase at a flow rate of 2.0 mL min–1. The effluent was monitored by UV detection at 254 nm. Calibration plots were linear in the range 160 to 240 μg mL–1 and the LOD and LOQ were 14.26 and 41.21 μg mL–1, respectively. The high recovery and low relative standard deviation confirm the suitability of the method for routine QC determination of oxaprozin in tablets.

Open access