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Summary
The composition of eggplant (Solanum melongena L.) leaf cuticular waxes was investigated. Chemical composition was determined as the first step in recognizing the importance to insect pest resistance of eggplant leaf surface chemistry. Waxes were extracted by dipping leaves into dichloromethane for 40 s. Relatively large samples were then fractionated by ‘flash chromatography’ on silica gel. Compounds were identified on the basis of their mass spectra from GC-MS analysis and retention data from GC-FID analysis, and quantified on the basis of peak areas from GC-FID analysis. Straight-chain alkanes and methyl-branched alkanes were the most abundant wax components. Minor quantities of esters of fatty acids with triterpene and aliphatic alcohols, free triterpene and aliphatic alcohols, sterols, and free fatty acids were also present on the eggplant leaf surface. This method is suitable for analysis of complex mixtures of plant cuticular lipids. It is also applicable to separation of samples for biological tests on insects.
Summary
Dauricine has a variety of pharmacological properties including anti-inflammatory, anti-arrhythmic, and antihypertensive effects as well as reversing multidrug resistance (MDR) of cancer cells. While its therapeutic application is increasing, its bioavailability of different administration routes has not been studied. In the present study, we developed and validated a liquid chromatography/electrospray ionization mass spectrometry method (LC-MS/MS). Using this method, we quantified dauricine in rat plasma after administration via intravenous (i.v.) injection, per oral (p.o.), and intraperitoneal injection (i.p.). Our results indicated that this method detected plasma dauricine with a good linearity in the range of 1.95–1000.00 ng/mL (r = 0.9997). The extraction method showed an average intra- and inter-day recovery of 98.21–104.35% and 98.0–103.58%, respectively. Dauricine showed a fast absorption and widespread distribution after administration in all three tested routes. After intravenous administration (2.5, 5.0, 10.0 mg/kg), the pharmacokinetics of dauricine exhibited a first-order kinetics. In addition, dauricine showed a slow elimination with a long half-life (t 1/2z) and double peaks phenomenon following p.o. and i.p. administration. Furthermore, using area under the plasma concentration-time curve (AUC), we calculated absolute bioavailability, which was over twofold higher when administered via i.p. than via p.o. administration. The newly obtained information from our study will provide important reference for dauricine dose and administration route in designing dauricine therapy for applicable diseases.
Abstract
Pharmaceuticals which are widely used in aquatic can easily migrate into the environment and aquatic animals, and can increase the risk of drug resistance and allergic symptoms if consumed by humans. In order to achieve high-throughput analysis of pharmaceuticals with different physical and chemical properties from complex matrices, we developed a new method for various types pharmaceuticals in fish and shrimp tissue. Series solid-phase extraction (s-SPE) with different adsorbents was selected for extracting and purifying analytes with different paddings. s-SPE were combined with ultra performance liquid chromatography triple quadruple tandem mass spectrometry (UPLC-MS/MS) for the detection of 30 pharmaceuticals antibiotics in fish samples. This method was stabilized and reliable to determinate the pharmaceuticals in fish and shrimp samples. As the method combined multiple Chinese national standards method, it could be easily treat the multi-pharmaceuticals from the fish and shrimp samples once time. It provided for both quantitative and qualitative methods and they could be applied to single- or multi-residue methods.
A talaj elektromos vezetőképessége és a termőhelyi zónák talajtulajdonságai közötti összefüggések
Correlations between soil conductivity and soil properties of crop management zones
Vizsgálatunk célja az volt, hogy egy Somogyban elhelyezkedő, dombvidéki mintaterület szántóin elemezzük a mért talaj-vezetőképesség (EC) értékek és lehatárolt termőhelyi (művelési) zónák talajtulajdonságai közötti összefüggéseket. A vizsgált szántóterületek löszön kialakult, típusos Ramann-féle barna erdőtalajon és karbonátos csernozjom barna erdőtalajon helyezkednek el. Feltalajuk döntően vályog és agyagos vályog fizikai féleségű. A talaj vezetőképességét 50 és 100 cm-es talajmélységben mértük.
A mintaterület talajadatait térinformatikai állományba foglaltuk, az adatok rendezését és azok összekapcsolását az ESRI ArcGIS 10.0 programmal végeztük el. A táblák heterogenitását mutató laboratóriumi talajvizsgálatok eredményeit a mért EC értékekkel összevetettük, amelyhez az IBM SPSS Statistics 20 szoftver segítségével stepwise-típusú lineáris regressziót alkalmaztunk. A regressziókat a talajvizsgálatok csoportosításával megegyezően: alap („a” eset), bővített („b” eset) és teljeskörű („c”eset) alapján futtattuk le. A számításoknál az „a” eset a talajtulajdonságokat meghatározó fontosabb talajparaméterek (kötöttség, humusz- és mésztartalom, kémhatás), a „b” eset az alap talajparamétereket és a makro tápanyagok (NPK ellátottságot), valamint a „c” eset az előző kettőt és mikro tápanyagok (Mg2+, Na+, Zn2+, Cu2+, Mn2+, SO4 2–, Fe2+ + Fe3+) körét jelenti.
A különböző csoportosításban elvégzett elemzések során arra voltunk kíváncsiak, hogy a vizsgálati talajparaméterek körének változtatásával szorosabb kapcsolatokat találunk-e a mért átlagos EC értékek és a talajtulajdonságok között. Az eredményeink által kaphatunk-e olyan kellő pontosságú és megbízhatóságú becslőmodellt, amely a talajok térbeli heterogenitását megmutatja az EC értékek alapján, így a módszer nagyban meggyorsíthatja és leegyszerűsítheti a „hagyományos” talajvizsgálatokhoz képest a termőhelyi zónák elkülönítését.
A vizsgálati eredményeink alapján elmondható, hogy mindhárom regressziós csoportosítás esetén a tengerszint feletti magasság csökkenésével arányosan nő a talaj-vezetőképesség, illetve az EC értékek növekedésével nő a talajok kötöttsége, amellyel együtt növekszik az agyagtartalom is. Ez a folyamat 100 cm-es talajmélységben a nagyobb víztartalom miatt erőteljesebben jelentkezik, mint az 50 cm-es talajmélységben. A termőhelyi zónák termékenységi viszonyait az elsődleges talajtulajdonságokon, illetve a makro és a mikro tápanyag-ellátottságokon kívül a domborzati viszonyok is módosíthatják. A talajellenállás mérése bárki számára elérhető, gyors és egyszerű módszer. A laboratóriumi talajvizsgálatokat kiegészítve alkalmas arra, hogy a precíziós növénytermesztésben segítséget nyújtson a termőhelyi zónák lehatárolásában.
Our aim was to analyse the relationships between the measured soil electrical conductivity (EC) and the soil properties of different delimited production (tillage) zones in a hillside sample area situated in Somogy county. The examined arable lands are situated in typical Ramann-type brown forest soil and chernozem-brown forest soil mostly with loam and clay loam formed on loess. For the investigations, two soil resistance values (measured at 50 cm and 100 cm depth) were used.
Soil data of the sample area were incorporated into a GIS file, the ordering and connection of the data was performed by ESRI ArcGIS 10.0 program. The results of the soil laboratory tests (which show soil heterogeneity) were correlated to the measured EC-values with stepwise linear regression using IBM SPSS Statistics 20 software. The regression were run in line with the alignment of soil investigations: basic (case „a”), extended (case „b”) and completed (case „c”). By the calculations, case „a” means the group of the most important soil parameters which are determinative soil characteristics (upper limit of plasticity or KA, humus-, lime content, pH), case „b” means the previous one plus the group of macronutrients (NPK-content), while case „c” means case „b” plus the group of micronutrients (Mg2+, Na+, Zn2+, Cu2+, Mn2+, SO4 2–, Fe2+ + Fe3+).
With the analyses made in different alignments our aim was to determine whether with the changing of examined soil parameters there will be tighter relationships between the measured EC-values and soil properties. Further aim was to examine whether it is possible to make a properly accurate and reliable estimation model, which can show the real soil circumstances (spatial heterogeneity of soils) based on EC-values, since this method can accelerate and simplify the separation of productivity zones compared to the conventional soil examinations.
Based on the results it can be concluded that in case of all the three regression groups the electrical conductivity increases proportionally with the decreasing of elevation. Besides, with the increasing of EC-values the KA – and with it, the clay content also – increases. This process develops in a more significant way in the depth of 100 cm than in 50 cm because of the higher water content. Besides the primary soil characteristics and the amount of macro- and micronutrients, the fertility conditions of the production zones can be affected by the geographical circumstances as well. The measurement of soil resistance is a fast, easy and generally available method, which is suitable – with the completion of laboratory examinations – for giving assistance to delineate the production zones in the precision crop production.
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